Human colon caner cells HCT116 (ATCC CCL-247) and SW480 cells (ATCC CCL-228) were purchased from ATCC. Cells were authenticated by Short-Tandem Repeat profiling (DSMZ, Braunschweig, Germany). All reagents were purchased from Sigma (Bornem, Belgium) unless mentioned otherwise. Antibodies were purchased from Sigma Life Sciences (Bornem, Belgium): myoferlin (HPA014245); Santa Cruz Biotechnology (Santa Cruz, CA): HSC70 (sc-7298), p21 (sc-6246), DRP-1 (sc-271583), Cyclin B1 (sc-752); Cell Signaling (Danvers, MA): phospho-DRP-1 (Ser616) (4494), phospho-p53 (Thr81) (2676), γH2A.X (9718), Cyclin D1 (2978); Merck-Millipore (Darmstadt, Germany): mitochondria (MAB1273), p53 clone BP53–12 (05–224); BD Biosciences: Cyclin A (611268), Cyclin E (551159).
Cyclin e
Manufactured by BD
Sourced in Germany, United States
Cyclin E is a lab equipment product that serves as a key regulator of the cell cycle. It plays a critical role in the transition from the G1 phase to the S phase of the cell cycle. Cyclin E binds to and activates the cyclin-dependent kinase 2 (CDK2) enzyme, which is essential for the initiation and progression of DNA replication.
Automatically generated - may contain errors
Lab products found in correlation
Sw480 cells atcc ccl 228, by American Type Culture Collection (1 mentions)
Hct116 atcc ccl 247, by American Type Culture Collection (1 mentions)
Cdk6 sc 177, by Santa Cruz Biotechnology (1 mentions)
Cdk4 sc 749, by Santa Cruz Biotechnology (1 mentions)
Anti β actin monoclonal antibody a5441, by Merck Group (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Cell counting kit 8 (cck8), by Dojindo Laboratories (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Metformin, by Sumitomo Pharma (1 mentions)
2 protocols using cyclin e
1
Characterization of Colon Cancer Cell Lines
2
Metformin Effects on Pancreatic Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chemicals. Metformin (1,1-dimethylbiguanide) was purchased from Dainippon Sumitomo Pharma Inc. (Osaka, Japan). The Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Laboratories (Kumamoto, Japan), and all other chemicals were obtained from Sigma Chemical Co. (Tokyo, Japan).
Antibodies. The antibodies used were anti-β-actin monoclonal antibody (A5441, used at 1:3,000; Sigma-Aldrich Co., St. Louis, MO, uSA), cyclin D1 (RB-9041, used at 1:1,000; Thermo Fisher Scientific K.K., Waltham, MA, USA), cyclin E (used at 1:1,000; BD Biosciences, San Jose, CA, uSA), CDK6 (sc-177, used at 1:1,000), CDK4 (sc-749, used at 1:2,500), phosphorylated retinoblastoma protein (Rb) (sc-50, used at 1:1,000) (all from Santa Cruz Biotechnology, Santa Cruz, CA, uSA), and secondary horseradish peroxidase-linked anti-mouse and anti-rabbit IgG antibodies (used at 1:2,000; GE Healthcare, Ltd., Buckinghamshire, uK).
Cell lines and culture. The human pancreatic cancer cell lines PK1, PK9 and Panc1 were obtained from the Japanese Cancer Research Resources Bank (Tokyo, Japan). Cells were grown in RPMI-1640 medium (Gibco Invitrogen, Carlsbad, CA, uSA) supplemented with 10% fetal bovine serum (FBS) (533-69545; Wako, Japan), and penicillin-streptomycin (100 mg/l; Gibco Invitrogen) in a humidified atmosphere of 5% CO 2 at 37˚C.
Antibodies. The antibodies used were anti-β-actin monoclonal antibody (A5441, used at 1:3,000; Sigma-Aldrich Co., St. Louis, MO, uSA), cyclin D1 (RB-9041, used at 1:1,000; Thermo Fisher Scientific K.K., Waltham, MA, USA), cyclin E (used at 1:1,000; BD Biosciences, San Jose, CA, uSA), CDK6 (sc-177, used at 1:1,000), CDK4 (sc-749, used at 1:2,500), phosphorylated retinoblastoma protein (Rb) (sc-50, used at 1:1,000) (all from Santa Cruz Biotechnology, Santa Cruz, CA, uSA), and secondary horseradish peroxidase-linked anti-mouse and anti-rabbit IgG antibodies (used at 1:2,000; GE Healthcare, Ltd., Buckinghamshire, uK).
Cell lines and culture. The human pancreatic cancer cell lines PK1, PK9 and Panc1 were obtained from the Japanese Cancer Research Resources Bank (Tokyo, Japan). Cells were grown in RPMI-1640 medium (Gibco Invitrogen, Carlsbad, CA, uSA) supplemented with 10% fetal bovine serum (FBS) (533-69545; Wako, Japan), and penicillin-streptomycin (100 mg/l; Gibco Invitrogen) in a humidified atmosphere of 5% CO 2 at 37˚C.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!