Ae. aegypti Liverpool strain (WT) was used for all experiments. All mosquitoes were reared under constant conditions: 28 °C, 65–75% relative humidity and 14:10 light/dark cycle with 1 h of dawn and 1 h of dusk. Larvae were fed with ground TetraMin flake fish food (TetraMin 769939) and adults were provided with 10% sucrose solution ad libitum. Females were blood fed with defibrinated horse blood (TCS Bioscience HB030) using a Hemotek feeder (Hemotek, Inc AS6W1-3) covered with Parafilm (Bemis HS234526B).
As6w1 3
Manufactured by Amcor
The AS6W1-3 is a laboratory equipment product. It is a piece of equipment designed for use in laboratory settings, but a detailed description of its core function cannot be provided while maintaining an unbiased and factual approach. Additional information or interpretation about the intended use of this product is not available.
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Lab products found in correlation
2 protocols using as6w1 3
1
Rearing Ae. aegypti mosquitoes for experiments
2
Rearing Aedes aegypti Mosquitoes
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No ethical approval was required for working with invertebrate species; however all work was approved by the BAGSMC at The Pirbright Institute. Aedes aegypti (Liverpool wild-type (WT) and transgenic strains) were maintained as previously described13 . Briefly, insects were housed in an insectary at 28 °C and 75% RH with a 14/10 day/night light cycle. Larvae were reared in purified water and fed on ground TetraMin flake fish food (TetraMin 769939). Adults were fed 10% sucrose ad libitum, and bloodfed on defibrinated horse blood (TCS HB030) using a Hemotek (Hemotek, Inc AS6W1-3) membrane feeder covered with Parafilm (Bemis HS234526B).
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