Biotin anti human cd63 antibody

Ultrapure and ultralow fatty acid content BSA was purchased from Nova Biologics. Formlabs standard clear resin (FLGPCL04) was purchased from Formlabs. Streptavidin (SA) was purchased from Iba (IBA GmbH, Germany), Methacrylic anhydride (94%), poly-(ethylene glycol) diacrylate (Mn = 575), Riboflavin (B2), Lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP), 5% Picrylsulfonic acid solution and 2,4,6-trinitrobenzenesulfonate (TNBS) were purchased from Sigma-Aldrich; Biotin Atto655 (ATTO-TEC GmbH, Germany), Biotin anti-human CD63 Antibody (BioLegend, USA), Biotin anti-human CD49 antibody - CD49e (Integrin alpha 5) Monoclonal Antibody (ThermoFisher, Austria), Mouse anti Human CD81 Antibody (Bio-Rad, Austria), Goat anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 647 were purchased ThermoFisher, Austria. Poly(l-Lysine) Poly(Ethylene Glycol) (PLL(20)-g[3.5]- PEG(2)) was purchased from SuSoS AG, Switzerland.

WT Jurkat and JinB8 T cells [14 (link)] were cultured using RPM1 1640 medium supplemented with 10% FBS (Gemini Bio, West Sacramento, CA, USA), glutamine and penicillin/streptomycin (Thermo Fischer Scientific, Waltham, MA, USA) as described previously [15 (link)]. Purified anti-human HLA-C Antibody, antibody, anti-human CD63 antibody, Biotin anti-human CD63 antibody, biotin anti-human HLA-A, B, C antibody, MHC-I-APC/640, CD63-PE and CD47-FITC antibodies were purchased from BioLegend, San Diego, CA, USA.

Aminopropytriethoxysilane (APTES), glutaraldehyde aqueous solution, IgG elution buffer, Tris buffer, bovine serum albumin (BSA), and hexamethyldisilazane (HMDS) were purchased from Sigma–Aldrich. Biotin anti-human CD63 antibody, FITC anti-human IgG antibody, and FITC anti-mouse IgG antibody were purchased from BioLegend. Exosome-free FBS was purchased from SunBio. The adenocarcinomic human alveolar basal epithelial cells (A549 cells) were purchased from China Infrastructure of Cell Line Resources (Beijing, China). PDMS was purchased from Dow Corning.