All the solvents used were analytical grade and purchased from Sigma–Aldrich. Anu tailam used in this research was procured from AVN Ayurveda Formulations Private Limited, Madurai, India. The crude sesame oil was procured from V.V.V.Anandham & Sons (India). The determination of viscosity was performed by using Brookfield viscometer (DV2T) and the refractive index was determined by Atago Refractometer (RX-5000 α). The moisture content was determined to confirm little or least presence of water in the formulation and was achieved through AND Moisture Balance (AND MS-70). The silica gel (60–120) for chromatography was purchased from Merck, India. The glass column fabricated with sintered glass filter was supplied by Borosil, India. Pre-coated silica gel GF254 aluminum plates for normal phase TLC were purchased from Merck, India to carry out HPTLC analysis on CAMAG HPTLC system using winCATS 1.4.7, Switzerland.
Refractometer rx 5000 α
Manufactured by Atago
The RX-5000 α is a refractometer, a device used to measure the refractive index of a liquid or solid sample. It provides precise measurements of the refractive index, which can be used to determine various properties of the sample, such as concentration, composition, or purity.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using refractometer rx 5000 α
1
Characterization of Ayurvedic Sesame Oil
2
Monitoring BSA Adsorption on Silica by MP-SPR
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Adsorption of BSA onto the SiO 2 surface was monitored using the optical method MP-SPR (BioNavis SPR NaviTM 200, BioNavis Ltd, Finland). The MP-SPR instrument with the Kretschmann configuration and angle-scanning goniometer is equipped with two different wavelengths (λ=670nm and λ=785nm) and two fluidic channels. The angular range is 40 to 78 degrees. Liquid flow conditions can be controlled with a peristaltic pump.
Changes in SPR angle were used to determine the mass adsorbed on the sensor surface according to the equation:
where ΔΘ is the change in the SPR angle, k is an instrument constant, d BSA is the thickness of the adsorbed BSA layer, and dn/dc is the refractive index increment; kxd BSA ≈1.0x10 -7 nm/deg for λ=670nm. The refractive index increment characterizes the change of the refractive index n with the concentration c, and was measured with an Atago Refractometer RX-5000α. For the BSA solution, dn/dc≈0.116cm 3 /g (with I=1x10 -2 M NaCl). We used a silica-coated MP-SPR sensor, which was cleaned using Hellmanex solution 0.5% (Hellma Analytics) and then rinsed with water before each experiment. The BSA layer was grown on the silica by flowing the protein solution (concentration 5ppm, I=1×10 -2 M NaCl, pH range 3.0 to 9.0) through the cell for 30min. The MP-SPR sensor was then rinsed with the electrolyte solution for 30min.
Changes in SPR angle were used to determine the mass adsorbed on the sensor surface according to the equation:
where ΔΘ is the change in the SPR angle, k is an instrument constant, d BSA is the thickness of the adsorbed BSA layer, and dn/dc is the refractive index increment; kxd BSA ≈1.0x10 -7 nm/deg for λ=670nm. The refractive index increment characterizes the change of the refractive index n with the concentration c, and was measured with an Atago Refractometer RX-5000α. For the BSA solution, dn/dc≈0.116cm 3 /g (with I=1x10 -2 M NaCl). We used a silica-coated MP-SPR sensor, which was cleaned using Hellmanex solution 0.5% (Hellma Analytics) and then rinsed with water before each experiment. The BSA layer was grown on the silica by flowing the protein solution (concentration 5ppm, I=1×10 -2 M NaCl, pH range 3.0 to 9.0) through the cell for 30min. The MP-SPR sensor was then rinsed with the electrolyte solution for 30min.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!