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Hemacolor stain set

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The Hemacolor stain set is a laboratory product used for staining blood smears to facilitate the microscopic examination of blood cells. It consists of a set of reagents that stain the different cellular components of blood, enabling the identification and analysis of various blood cell types.

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Lab products found in correlation

Biocoat matrigel invasion inserts, by BD (2 mentions) Matrigel, by Corning (1 mentions) Transwell insert, by Corning (1 mentions) Opti mem, by Thermo Fisher Scientific (1 mentions) Opti mem, by Corning (1 mentions)

Close spelling variants of this product

Hemacolor (80 citations) Hemacolor stain (3 citations) Hemacolor-Set (2 citations) Harleco Hemacolor Stain Set (2 citations)

4 protocols using hemacolor stain set

1

Quantifying Cell Invasion Capacity

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5 × 104 cells were resuspended in serum-free and glucose-free DMEM and seeded into the interior of BD BioCoat Matrigel Invasion inserts (n=3/group). Once the cells were seeded, DMEM supplemented with 20% FBS was placed into the lower well as the chemoattractant. After 22 h incubation, non-invasive cells were removed from the upper surface, and the remaining cells were stained with crystal violet or the Hemacolor stain set (EMD Millipore) and then quantified.
Tanaka N., Zhao M., Tang L., Patel A.A., Xi Q., Van H.T., Takahashi H., Osman A.A., Zhang J., Wang J., Myers J.N, & Zhou G. (2017). Gain-of-function mutant p53 promotes the oncogenic potential of head and neck squamous cell carcinoma cells by targeting the transcription factors FOXO3a and FOXM1. Oncogene, 37(10), 1279-1292.
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2

Matrigel Invasion Assay for Cell Migration

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In total 5 × 104 cells were resuspended in serum-free and glucose-free DMEM and seeded into the interior of BD BioCoat Matrigel Invasion inserts (n = 3/group). Once the cells were seeded, DMEM supplemented with 20% FBS was placed into the lower well as the chemoattractant. After 22 h incubation, non-invasive cells were removed from the upper surface, and the remaining cells were stained with crystal violet or the Hemacolor stain set (EMD Millipore, Burlington, MA, USA) and then quantified.
Tanaka N., Zhao M., Tang L., Patel A.A., Xi Q., Van H.T., Takahashi H., Osman A.A., Zhang J., Wang J., Myers J.N, & Zhou G. (2017). Gain-of-function mutant p53 promotes the oncogenic potential of head and neck squamous cell carcinoma cells by targeting the transcription factors FOXO3a and FOXM1. Oncogene, 37(10), 1279-1292.
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3

Cell Migration and Invasion Assay

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For our migration assay, HCT-116 cells (2 × 105 cells) in 100 μl of OPTI-MEM (Gibco, 51985034) were plated into a 0.8-μm pore sized 24-well Transwell inserts (Corning, 3422) and 600 μl of OPTI-MEM with 10% FBS was added to a lower chamber. For invasion assay, Transwell inserts were coated with 10% Matrigel (Corning, 356231) before cell seeding. After 24 hours, cells were stained with Hemacolor Stain Set (Sigma-Aldrich, 65044) according to manufacturer’s instruction. Non-migrating or -invading cells were removed using a wet cotton swab from the top surface of membrane. Images of migrating or invading cells on the bottom surface of membrane were captured on 100× fields (Olympus, IX71). Cell numbers were counted in at least six fields per insert from four independent experiments.
Choi S.H., Kim J.K., Chen C.T., Wu C., Marco M.R., Barriga F.M., O’Rourke K., Pelossof R., Qu X., Chang Q., de Stanchina E., Shia J., Smith J.J., Sanchez-Vega F, & Garcia-Aguilar J. (2022). KRAS Mutants Upregulate Integrin β4 to Promote Invasion and Metastasis in Colorectal Cancer. Molecular cancer research : MCR, 20(8), 1305-1319.
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4

Differential Staining of Cervical Smears

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Cervical smears collected throughout the observation period were differentially stained with Hemacolor Stain Set according to the manufacturer’s protocol (Sigma-Aldrich, Burlington, MA). PMNs were counted in five nonadjacent high-power fields (400x) and averaged.
Aguila L.K., Patton D.L., Gornalusse G.G., Vojtech L.N., Murnane R.D, & Wood G.E. (2022). Ascending Reproductive Tract Infection in Pig-Tailed Macaques Inoculated with Mycoplasma genitalium. Infection and Immunity, 90(6), e00131-22.
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