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3 protocols using acetoin

1

Volatile Compounds Analysis Protocol

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Diethyl ether, sodium bicarbonate, hydrochloric acid, and sodium sulfate were obtained from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). Serial n-alkanes (C7-C40) were purchased from O2si Smart Solutions (North Charleston, SC). The standards butanoic acid (99%), furaneol (98%), acetic acid (99%), acetoin (98%), methional (99%), 2-heptanone (98%), and homofuraneol (98%) were purchased from Macklin Biochemical Co. Ltd. (Shanghai, China) . Hexanoic acid (99%), ethyl caprylate (98%), ethyl caprate (99%), hexanal (98%), furfural (98%), 2-nonanone (99%), (E)-2-nonenal (99%), ethyl hexanoate (98%), phenethyl alcohol (99%), ethyl butanoate (99%), 2,3-butanedione (98%), nonanal (99%), octanoic acid (99%), limonene (95%), methanethiol (2,000 μg/mL in toluene), octanal (99%), 2-undecanone (99%), and dimethyl trisulfide (98%) were obtained from Sigma-Aldrich AccuStandard (Beijing, China).
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2

Volatile Compound Identification Standards

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Standards were purchased for volatile compound identification. 2-Pentylfuran and trans-3-octen-2-one were provided by Sigma Aldrich (St. Louis, MO, USA). Other compounds, including hexanal, benzaldehyde, octanal, nonanal, heptanal, 3-methylbutanal, propanol, pentanol, hexanol, ethanol, 1-octen-3-ol, 3-methyl-1-butanol, 2-methylpropanol, phenethyl alcohol, acetoin, 6-methyl-5-hepten-2-one, 2,3-butanedione, acetic acid, octanoic acid, pentanoic acid, hexanoic acid, γ-undecalactone, ethyl acetate, hexyl acetate, were provided by Macklin (Shanghai, China).
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3

Extraction and Preservation of Coconut Water Samples

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Sodium chloride (99.5 %), 2-methyl-3-heptanone (99 %), 1-heptanol (99.99 %), ethyl acetate (99.99 %), ethyl caproate (99.99 %), ethyl octanoate (99.5 %), isovaleraldehyde (99.99 %), octanal (99.99 %), 1-nonanal (99.99 %), 2,3-butanedione (99.99 %), acetoin (99.99 %), ethanol (99.99 %), 1-propanol (99.5 %) and acetaldehyde (99.99 %) were obtained from Macklin Biochemical Co. Ltd. 2-Acetyl-1-pyrroline (10 %w/w in Toluene) and n-alkane (C7-C40) were purchased from Sigma–Aldrich Chemical Co. (St. Louis, U.S.A.). Wenye No. 4 was an aromatic coconut with a maturity period of approximately 8 months harvested from the coconut germplasm repository of the Ministry of Agriculture and Rural Affairs in Wenchang, China. THD were harvested from the Thailand Aromatic Green Dwarf from Samut Sakhon Province, Thailand, with a ripeness of 7–8 months. Twenty coconuts of each variety were picked randomly at the planting site in January 2022. The coconuts were transported to the laboratory immediately after harvesting. Fresh coconut water was extracted, filtered and encapsulated in 50 mL centrifuge tubes immediately under sterile conditions. Packed samples were prefrozen at −20 ℃ for 24 h and then stored at −80 ℃ to maintain the original properties.
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