Live imaging was performed on sedated Lyve1CreERTtdT mice. The mouse was positioned laterally and the globe exposed by retracting the eyelids to expose the limbus near the lateral canthus. Images were obtained using a Leica MZ10F Fluo III microscope using a Leica Planap 1.0× objective and a Leica DFC310FX camera (acquisition software: LAS version 4.0.0.8777) or a Ziess Axio Zoom.V16 and a Zeiss Plan-NeofluarZ 1.0 0.25 na objective and a Zeiss AxioCam MRm camera (acquisition software: Zeiss Zen 2012, blue edition, version 1.1.1.0).
Dfc310fx camera
Manufactured by Zeiss
The DFC310FX is a digital camera designed for microscopy applications. It features a 3.2-megapixel CMOS sensor and supports image capture at up to 30 frames per second. The camera is equipped with a USB 3.0 interface for high-speed data transfer.
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3 protocols using dfc310fx camera
1
Live Imaging of Lymphatic Vessels
2
Live Imaging of Lymphatic Vessels
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Live imaging was performed on sedated Lyve1CreERTtdT mice. The mouse was positioned laterally and the globe exposed by retracting the eyelids to expose the limbus near the lateral canthus. Images were obtained using a Leica MZ10F Fluo III microscope using a Leica Planap 1.0× objective and a Leica DFC310FX camera (acquisition software: LAS version 4.0.0.8777) or a Ziess Axio Zoom.V16 and a Zeiss Plan-NeofluarZ 1.0 0.25 na objective and a Zeiss AxioCam MRm camera (acquisition software: Zeiss Zen 2012, blue edition, version 1.1.1.0).
3
Intravital Imaging of Mouse Pinna Lymphatics
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Live imaging was performed on sedated Lyve1CreERTtdT mice. The pinna was depilated and placed between glass slides, and the mouse was positioned laterally. Images were obtained using a Leica MZ10F Fluo III microscope using a Leica Planap 1.0X objective and a Leica DFC310FX camera (acquisition software: LAS version 4.0.0.8777) or a Ziess Axio Zoom.V16 and a Zeiss Plan-NeofluarZ 1.0 × 0.25 na objective and a Zeiss AxioCam MRm camera (acquisition software: Zeiss Zen 2012, blue edition, version 1.1.1.0). To acquire the clonal and small population LEC data, we used low magnification light microscopy to identify the major blood vessels within the pinna. These large stable structures were used to develop a vascular map of the pinna. Using this information to provide image guidance and the identical power of magnification, we were able to visualize the same fields of interest using light and fluorescent microscopy.
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