Hms 740

Manufactured by Thermo Fisher Scientific

Sourced in Germany

The HMS 740 is a high-speed microplate shaker designed for efficient mixing of samples in microplates. It features a compact and durable construction, with a shaking speed range of 150 to 1,500 rpm and a shaking diameter of 3 mm. The HMS 740 is suitable for use in various laboratory applications.

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Lab products found in correlation

Hematoxylin, by Bio-Optica (1 mentions) Eosin y, by Bio-Optica (1 mentions)

2 protocols using hms 740

Histological Analysis of Vascular Cell Co-Culture

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After 1 month of in vitro co-culture, ECs and SMCs on the tubular scaffolds were analysed via H&E staining. The scaffold was first washed with PBS and fixed with 4% PF. Then, the scaffold was cut crosswise to obtain a 5 μm thick section using a microtome. Afterwards, H&E staining was performed using an automatic stainer (Micron HMS 740, Thermo Scientific, Germany) according to standard histological procedures. Finally, the section was mounted for observation under a laser scanning quantitative imaging system (VECTRA, PerkinElmer, USA).

Li M.X., Li L., Zhou S.Y., Cao J.H., Liang W.H., Tian Y., Shi X.T., Yang X.B, & Wu D.Y. (2021). A biomimetic orthogonal-bilayer tubular scaffold for the co-culture of endothelial cells and smooth muscle cells. RSC Advances, 11(50), 31783-31790.

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Hematoxylin and Eosin Staining

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The samples for H&E staining protocol were automatically processed (HMS740, Microm, ThermoFisher Scientific). Briefly, histological sections were subsequently immersed in hematoxylin (BioOptica) and after bluing in an ammonia solution (1% v/v), in eosin y (BioOptica).

Correia C.R., Santos T.C., Pirraco R.P., Cerqueira M.T., Marques A.P., Reis R.L, & Mano J.F. (2017). In vivo osteogenic differentiation of stem cells inside compartmentalized capsules loaded with co-cultured endothelial cells. Acta biomaterialia, 53.

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