Acrylamide, bisacrylamide and Hybond™-P membrane were purchased from Amersham Bioscience/GE Healthcare (Little Chalfont, UK). IPG buffers, 18 cm ReadyStrip IPG strips (pH 5–8), 18 cm ReadyStrip IPG strips (pH 3.0-5.6), 18 cm ReadyStrip IPG strips (pH 5.3-6.5), and Electrode Wicks were from Bio-Rad Laboratories (Marnes la Coquette, France), and orthophosphoric acid, ammonium sulphate and absolute ethanol were from VWR (Strasbourg, France). All other chemicals were from Sigma (L’Isle-d’Abeau Chesnes, France). Sequence grade-modified trypsin was purchased from Promega (Charbonnières-les-bains, France), and Luminata™ Western Horseradish peroxidase (HRP) substrate and ReBlot Plus Strong antibody stripping solution were from Millipore (Molsheim, France). For immunoblotting, the monoclonal antibodies against VCL and FHL3 were from Sigma. The anti-mouse IgG-HRP, anti-rabbit IgG-HRP, anti-SRL and anti-β-actin (ACTB) were from Santa-Cruz biotechnology (Heidelberg, Germany). The polyclonal antibodies against GPD1, MYOZ1, ANXA1, ANXA5, GSTO1, HSPB5, HSPC3, ALDH2, NDUFS2, UQCRFS1, ENO3, HSPA1A and VCP were purchased from Euromedex (Genetex, Souffelweyersheim, France).
Gueugneau M., Coudy-Gandilhon C., Gourbeyre O., Chambon C., Combaret L., Polge C., Taillandier D., Attaix D., Friguet B., Maier A.B., Butler-Browne G, & Béchet D. (2014). Proteomics of muscle chronological ageing in post-menopausal women. BMC Genomics, 15, 1165.
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