1 2 bis 2 aminophenoxy ethane n n n n tetraacetic acid acetoxy methyl bapta am

The chemicals used in the culture of cells were acquired from Gibco ® (Gaithersburg, MD, USA). Aminopolycarboxylic acid/acetoxy methyl (fura-2/AM) and 1,2-bis(2-aminophenoxy) ethane-N,N,N',N'tetraacetic acid/acetoxy methyl (BAPTA/AM) were purchased from Molecular Probes ® (Eugene, OR, USA). All other compounds were purchased from Sigma-Aldrich ® (St. Louis, MO, USA).

The chemicals for cell culture were purchased from Gibco ® (Gaithersburg, MD, USA). Aminopolycarboxylic acid/acetoxy methyl (fura-2/AM) and 1,2-bis (2-aminophenoxy) ethane-N, N, N', N'-tetraacetic acid/acetoxy methyl (BAPTA/AM) were acquired from Molecular Probes ® (Eugene, OR, USA). The other chemicals were purchased from Sigma-Aldrich ® (St. Louis, MO, USA).

3,4-DMP (Figure 1(a)) were from Sigma-Aldrich ® (St. Louis, MO, USA). Phorbol 12-myristate 13 acetate [PMA, 1 nM, a protein kinase C (PKC) activator], GF109203X (2 mM, a PKC inhibitor), modulators of store-operated Ca 2þ channels (econazole, 0.5 mM; 2-APB, 20 mM; SKF96365, 5 mM), U73122 [2 mM, a phospholipase C (PLC) inhibitor], adenosine triphosphate (ATP, 4 mM, a PLC-dependent agonist), or thapsigargin (TG, 1 mM, an endoplasmic reticulum Ca 2þ pump inhibitor) were from Sigma-Aldrich ® (St. Louis, MO, USA) unless otherwise indicated. Aminopolycarboxylic acid/ acetoxy methyl (fura-2-AM, 2 mM) and 1,2-bis(2aminophenoxy)ethane-N, N, N',N'-tetra acetic acid/acetoxy methyl (BAPTA-AM, 5 mM) were from Molecular Probes ® (Eugene, OR, USA). The reagents for cell culture were from Gibco ® (Gaithersburg, MD, USA).