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2 protocols using xylene

1

Inflammatory Pathway Modulation Protocol

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Chloral hydrate, xylene and Wright-Giemsa stain were purchased from Nanjing Chemical Reagent Co. (Nanjing, China). HPD-100 macroporous resin was purchased from Tianjin Haiguang Chemical Co., Ltd. (Tianjin, China). Dexamethasone acetate, lipopolysaccharide (LPS), and other compounds with the purity >95% were purchased from the National Institute for Food and Drug Control (Beijing, China). HPLC-grade acetonitrile, methanol and formic acid were supplied by Merck (Darmstadt, Germany). BCA protein assay kit, mouse myeloperoxidase (MPO), TNF-α, IL-1β, IL-6 and VEGFA ELISA kit were purchased from Proteintech Group Inc. (Chicago, IL, USA).
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2

Dehydroabietic Acid Synthesis Protocol

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Dehydroabietic acid (DA) was obtained from Wuzhou Chemical Co., Ltd. Benzocyclobutene-4-boronic acid was purchased from Chemtarget Technologies Co., Ltd. N-Bromosuccinimide (NBS), tripotassium phosphate, Tetrakis(triphenylphosphine)palladium(0), allyl bromide, potassium carbonate and azodiisobutyronitrile (AIBN) were obtained from Aladdin Industrial Corporation. Ethanol, acetonitrile, acetone, xylene, ethyl acetate and petroleum ether were purchased from Nanjing Chemical Reagent Co., Ltd. All chemicals were used without purification.
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