Dulbecco modified eagle medium (dmem)

The murine pancreatic cancer cell line, developed from the tumour tissues of Kras^LSL-G12D/+, Trp53^LSL-R172H/+, Pdx1-Cre(KPC) mice that can develop spontaneous pancreatic ductal adenocarcinoma (PDAC), was kindly provided by Dr. Tingbo Liang’s Research Group (Zhejiang University, China) and maintained in RPMI-1640 medium (Gibco, Shanghai, China), supplemented with 20% foetal bovine serum (FBS), 1% sodium pyruvate (100×), and 1% MEM Non-Essential Amino Acids (NEAA 100×). The murine pancreatic ductal epithelial cell line (MPDC) was purchased from CELLBIO Company (CBR131654, Shanghai, China) and cultured in Dulbecco’s modified Eagle’s Medium containing L-Glutamine, 4.5 g/L D-Glucose, and 110 mg/L sodium pyruvate (DMEM; Gibco, Shanghai, China) and supplemented with 10% FBS. C2C12 myoblasts were purchased from the cell bank of the Chinese academy of sciences (GNM26, Shanghai, China) and cultured in DMEM, supplemented with 10% FBS. When C2C12 myoblasts achieved 70–80% confluence, the medium was replaced with DMEM containing 2% horse serum daily, which would induce the differentiation of myoblasts into myotubes in 4–6 days. The myotubes were used for the experiments. The images of the C2C12 differentiation process were obtained by fluorescence microscopy (40×, Carl Zeiss, Germany) in coordination with an imaging system (Ckx41, Olympus).

Fmoc-protected amino acids were purchased from Shanghai GL Biochem Ltd (shanghai, China). HCTU, HOBt, Oxyma were obtained from damas-beta (shanghai, China). Et₂O, NMP, TFA, TFE, DMF, DCM, DIPEA, TIPS, piperidine, phenol, dimethylsulfoxide and other common reagent were purchased from Sinopharm Chemical Reagent Co. Ltd (shanghai, China). Purification of crude peptides was carried out by using HPLC LC-20AD SHIMADZU with dual wavelength (214 and 254 nm) (Japan). The Purity detection of peptides by using HPLC Thermo UltiMate 3000 Series (USA). Rink Amide MBHA resin (0.33 mmol g⁻¹ loading) was purchased from Tianjin Nankai Hecheng Science & Technology Co. Ltd (Tianjing, China). Trypsin was purchased from TCI (Japan). All other commercially obtained reagents and solvents were used directly without further purification. Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), penicillin, and streptomycin were purchased from Gibco (Grand Island, NY, USA). The tumor cells A549, HepG2, HCT116 and normal cells LO-2, BEAS-2 293 T were obtained from the Cell Bank of Chinese Academy of Sciences (Shanghai, China), and cultured in DMEM supplemented with 10% FBS, 100 units per mL of penicillin and 100 μg mL⁻¹ of streptomycin. All of the cells were incubated at 37 °C in an atmosphere of 5% CO₂.

Fetal bovine serum (FBS), Dulbecco’s modified Eagle medium (DMEM) and penicillin/streptomycin were purchased from Gibco (GrandIsland, State of New York, USA).The hepatocarcinoma cell lines HepG2, Huh7, HepG2.2.15 cells (derived from HepG2 cells carrying HBV genome) and HepAD38 (replicates HBV under conditions regulated with tetracycline) were obtained from China Center for Type Culture Collection (CCTCC) and grown in DMEM supplemented with 10% FBS, 100 U/mL penicillin, and 100 µg/mL streptomycin. The cells were seeded in 24-well (seeding densities: 2.5 × 10⁵ cells per well), 12-well (seeding densities: 5 × 10⁵ cells per well), 6-well (seeding densities: 1.2 × 10⁶ cells per well), and 6 cm vessels (seeding densities: 2.6 × 10⁶ cells per dish), and were transfected by Lipofectamine 2000 transfection regent following the manufacturer’s instructions.