Rwpe 1 normal prostate cell line

Prostate cancer and matched paracancerous samples were collected from operated specimens of prostate cancer patients from the Department of Urology, Second Hospital of Tianjin Medical University, and stored in liquid nitrogen as soon as possible after excision. Our research had the approval of the ethics committee of the Second Hospital of Tianjin Medical University. The human ethical approval number is KY2023K144. Each patient was the subject of an informed consent form. The human prostate cancer cell lines PC-3, DU-145, LNCaP, C4-2 and the RWPE-1 normal prostate cell line were obtained from the American Type Culture Collection. All of these cells have been frozen, revived and grown in culture by the Tianjin Institute of Urology. All cell lines were incubated in DMEM or RPMI 1640 medium (Gibco BRL Life Technologies, Waltham, MA, USA) which contained 10% FBS (Gibco BRL Life Technologies) and 1% antibiotics (Gibco BRL Life Technologies). The cells were all cultured in a humidified incubator at 37°C with 5% CO₂.

DU145 (both variants herein termed DU-L [E-cadherin low expressing] and DU-H [E-cadherin high expressing]), PC3 (both variants herein termed PC3-L [E-cadherin low expressing] and PC3-H [E-cadherin high expressing]) and LnCaP human prostate cancer cell lines, and RWPE-1 normal prostate cell line, were purchased from the American Type Culture Collection (Manassas, VA, USA). DU-H/DU-L and PC3-H/PC3-L cells were maintained in DMEM and F-12 K media, respectively. LnCaP cells were maintained in RPMI 1640 media and RWPE-1 cells in Keratinocyte Serum Free Medium (K-SFM). Cells were seeded into 6-well plates and after 24 h treated with IFNγ (5 ng/mL) or control (PBS) for 48 h. Cells were harvested for immunoblot or flow cytometry, or fixed for immunofluorescence.