Immunofluorescence was used to detect F4/80, CD45.1, and CD45.2 as described by us previously [13 (link)]. Briefly, frozen livers were cut into 8 μm sections, fixed in 4% formalin for 10 minutes, followed by blocking in 10% goat serum. The sections were then incubated with Alexa Fluor-488 anti-mouse CD45.1 antibody (Biolegend) diluted 1:100, Alexa Fluor-488 anti-mouse CD45.2 antibody (Biolegend) diluted 1:100, or rat anti-F4/80 antibody (Bio-Rad, Hercules, CA) diluted 1:500. The sections were then incubated with goat anti-rat secondary antibody conjugated to Alexa Fluor-594 diluted 1:500 (Thermo-Fisher Scientific).
Alexa fluor 488 anti mouse cd45.1 antibody
Manufactured by BioLegend
The Alexa Fluor-488 anti-mouse CD45.1 antibody is a fluorescently labeled monoclonal antibody that specifically binds to the CD45.1 allotypic determinant on mouse cells. It can be used for the identification and enumeration of CD45.1-expressing cells in flow cytometry applications.
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2 protocols using alexa fluor 488 anti mouse cd45.1 antibody
1
Immunofluorescence Analysis of Liver Cells
2
Immunofluorescence Analysis of Liver Cells
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Immunofluorescence was used to detect F4/80, CD45.1, and CD45.2 as described by us previously [13 (link)]. Briefly, frozen livers were cut into 8 μm sections, fixed in 4% formalin for 10 minutes, followed by blocking in 10% goat serum. The sections were then incubated with Alexa Fluor-488 anti-mouse CD45.1 antibody (Biolegend) diluted 1:100, Alexa Fluor-488 anti-mouse CD45.2 antibody (Biolegend) diluted 1:100, or rat anti-F4/80 antibody (Bio-Rad, Hercules, CA) diluted 1:500. The sections were then incubated with goat anti-rat secondary antibody conjugated to Alexa Fluor-594 diluted 1:500 (Thermo-Fisher Scientific).
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