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Annexin 5 pi apoptosis assay kit

Manufactured by Thermo Fisher Scientific
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The Annexin V-PI apoptosis assay kit is a laboratory instrument used to detect and quantify apoptosis, a programmed cell death process. The kit utilizes Annexin V, a protein that binds to phosphatidylserine, and propidium iodide, a nucleic acid stain, to distinguish between apoptotic, necrotic, and viable cells.

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Lab products found in correlation

Alamarblue assay, by Thermo Fisher Scientific (1 mentions) Facsaria, by BD (1 mentions) Deadend fluorometric tunel system, by Promega (1 mentions) Caspase glo 3 7 assay kit, by Promega (1 mentions)

4 protocols using annexin 5 pi apoptosis assay kit

1

Apoptosis Assessment by Flow Cytometry

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Cells were processed using an AnnexinV-PI apoptosis assay kit (Life Technologies) and analyzed total 20,000 events by flow cytometry.
Yanagi T., Krajewska M., Matsuzawa S.I, & Reed J.C. (2014). PCTAIRE1 phosphorylates p27 and regulates mitosis in cancer cells. Cancer research, 74(20), 5795-5807.
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2

Cytotoxicity Evaluation of Magnetic Nanoparticles

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Cell viability was studied by Alamar Blue assay (Invitrogen) and FITC-annexin V/propidium iodide staining. In the former, either the murine NK cells expanded in vitro in the presence of IL-2 or NK-92MI cells were incubated with different MNP concentrations for 24 h, after which Alamar Blue was added and then cultured for an additional 4 h, at which point fluorescence was measured. For the second assay, cells were processed using the Annexin V-PI apoptosis assay kit following the manufacturer's protocol (Life Technologies) and analyzed by flow cytometry.
Sanz-Ortega L., Rojas J.M., Portilla Y., Pérez-Yagüe S, & Barber D.F. (2019). Magnetic Nanoparticles Attached to the NK Cell Surface for Tumor Targeting in Adoptive Transfer Therapies Does Not Affect Cellular Effector Functions. Frontiers in Immunology, 10, 2073.
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3

Apoptosis Evaluation by Flow Cytometry

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Cells were processed using the Annexin V-PI apoptosis assay kit (FITC-annexin V/propidium iodide staining) according to the manufacturer's protocol (Life Technologies) and 20,000 events were analyzed by flow cytometry using FACS Aria (Becton Dickinson).
Yanagi T., Nagai K., Shimizu H, & Matsuzawa S.I. (2017). Melanoma antigen A12 regulates cell cycle via tumor suppressor p21 expression. Oncotarget, 8(40), 68448-68459.
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4

Detecting Adipocyte Apoptosis by Multiple Assays

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Apoptosis in adipocytes was observed and detected by various methods, including caspase 3/7 activation analysis, TUNEL assay, and flow cytometry. Caspase 3/7 activity was quantitated by using a Caspase-Glo® 3/7 Assay Kit (Promega, CA, USA) according to the manufacturer's instructions. A DeadEnd™ Fluorometric TUNEL System (Promega) was used to observe and measure the adipocyte apoptosis. The number of apoptotic adipocytes was quantitated by flow cytometry assay (Gallios, Beckman Coulter, USA) with the Annexin V/PI double-staining method according to the manufacturer's instructions. (The Annexin V/PI apoptosis assay kit was purchased from Life Technologies, USA).
Qi R., Wang Q., Wang J., Huang J., Jiang S., Xiao R., Liu Z, & Yang F. (2016). Expression Pattern and Regulatory Role of microRNA-23a in Conjugated Linoleic Acids-Induced Apoptosis of Adipocytes. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 40(3-4).
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