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2 protocols using rip2 pab

1

Antibodies and Reagents for NF-κB Signaling

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Polyclonal antibodies generated against DREAM, ICAM-1, USF1, TRAF2, TRAF6, NF-κB1, NF-κB2, RelB, c-Rel, and IκBα were obtained from Santa Cruz Biotechnology, Inc. Anti-A20 mouse monoclonal antibody (mAb; 59A426) was from Calbiochem. c-Fos polyclonal antibody (pAb), phospho-TAK1 (Thr184/187) pAb, TAK1 rabbit mAb (D94D7), phospho-p38 MAPK (Thr/Tyr182) mAb (28B10), p38 MAPK pAb, phospho-SAPK/JNK (Thr183/Tyr185) rabbit mAb (81E11), SAPK/JNK rabbit mAb (56G8), IKKα pAb, phospho-IKKα/β (Ser176/180) pAb, IKKβ pAb, phospho-IκBα (Ser32) rabbit mAb (14D4), RIP1 pAb, and RIP2 pAb were from Cell Signaling. IKKγ mAb (clone 72C627) and DREAM mAb (clone 40A5) were obtained from Upstate. p65/RelA pAb was from Chemicon. Control siRNA was from Qiagen and hUSF1-siRNA (SMARTpool, cat # L003617) was from Dharmacon. siRNA transfection reagent was obtained from Santa Cruz Biotechnology, Inc. Lipids (dimethyldioctadecylammonium bromide and chlolesterol) for liposome preparation and anti-β-actin mAb (clone AC-15) were purchased from Sigma. PCR primers were custom-synthesized from Integrated DNA Technologies.
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2

Antibodies and Reagents for NF-κB Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Polyclonal antibodies generated against DREAM, ICAM-1, USF1, TRAF2, TRAF6, NF-κB1, NF-κB2, RelB, c-Rel, and IκBα were obtained from Santa Cruz Biotechnology, Inc. Anti-A20 mouse monoclonal antibody (mAb; 59A426) was from Calbiochem. c-Fos polyclonal antibody (pAb), phospho-TAK1 (Thr184/187) pAb, TAK1 rabbit mAb (D94D7), phospho-p38 MAPK (Thr/Tyr182) mAb (28B10), p38 MAPK pAb, phospho-SAPK/JNK (Thr183/Tyr185) rabbit mAb (81E11), SAPK/JNK rabbit mAb (56G8), IKKα pAb, phospho-IKKα/β (Ser176/180) pAb, IKKβ pAb, phospho-IκBα (Ser32) rabbit mAb (14D4), RIP1 pAb, and RIP2 pAb were from Cell Signaling. IKKγ mAb (clone 72C627) and DREAM mAb (clone 40A5) were obtained from Upstate. p65/RelA pAb was from Chemicon. Control siRNA was from Qiagen and hUSF1-siRNA (SMARTpool, cat # L003617) was from Dharmacon. siRNA transfection reagent was obtained from Santa Cruz Biotechnology, Inc. Lipids (dimethyldioctadecylammonium bromide and chlolesterol) for liposome preparation and anti-β-actin mAb (clone AC-15) were purchased from Sigma. PCR primers were custom-synthesized from Integrated DNA Technologies.
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