After animals were anesthetized with isoflurane, two-dimensional (2D) chest x-ray images (In-Vivo Multispectral Imaging System FX, Bruker) were obtained, and microCT scans (Inveon, Siemens) were performed with respiratory gating applied during image acquisition to minimize breathing motion artifacts. The Inveon Research Workplace (Siemens) general analysis and 3D Visualization software were used for image review and analysis and to produce 3D volume-rendered images. For postmortem CT studies, mice were sacrificed by intraperitoneal injection of Fatal-Plus solution (Vortech), intratracheally intubated with a 20-gauge plastic catheter, and inflated with air to a pressure of 20 cm H2O.
In vivo multispectral imaging system fx
Manufactured by Bruker
The In-Vivo Multispectral Imaging System FX is a versatile lab equipment designed for advanced biological and biomedical imaging applications. It utilizes multispectral imaging technology to capture high-resolution images and data across a wide range of wavelengths, providing comprehensive information about the sample under investigation.
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Lab products found in correlation
2 protocols using in vivo multispectral imaging system fx
1
In-Vivo Lung Imaging and Analysis
2
Microcomputed Tomography for Lung Stone Burden
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After animals were anesthetized with isoflurane, two-dimensional (2D) chest x-ray images (In Vivo Multispectral Imaging System FX, Bruker) were obtained, and microCT scans (Inveon, Siemens) with respiratory gating were performed. For microlith burden assessment, a commercial graphic processing software package (Amira, FEI Company, Hillsboro OR) was used to segment the lung from other soft tissues in CT images. Mean lung density and percentage of high-density lung volumes (>1.5 g/cc and >1.25 g/cc, calculated from the Hounsfield units in CT images) were calculated from the entire lung in MATLAB. Total mass of the lung was calculated by multiplying average density by the CT-derived lung volume. Stone burden was calculated as the product of lung volume and the increase in lung density over the average density of wild-type lungs.
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