Prior to cell stimulation or infection, cells were treated with the indicated concentrations of MAPK inhibitors (Tocris) diluted in dimethyl sulfoxide (DMSO) for 1 h. Inhibitors remained present throughout the experiment.
Mapk inhibitors
Manufactured by Bio-Techne
Sourced in United States
MAPK inhibitors are a class of chemical compounds that specifically target and inhibit the activity of mitogen-activated protein kinases (MAPKs). MAPKs are a family of enzymes involved in various cellular processes, including cell growth, differentiation, and response to environmental stimuli. MAPK inhibitors can be used as research tools to study the role of MAPK signaling pathways in biological systems.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mapk inhibitors
1
MAPK Inhibitor Pre-treatment Protocol
2
Microbial Erythritol Lipid Synthesis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The MELs used in the present study were produced and provided by DKBIO (Daejeon, Korea). Briefly, MELs were synthesized in a yeast strain of Pseudozyma sp., extracted from the culture broth with ethyl acetate, and purified through a fatty acid removal process. Purified MELs were dissolved with dimethyl sulfoxide for use in the experiments. MELs are mixtures consisting of MEL-A, MEL-B, MEL-C, and MEL-D. The MELs used in this study are composed of predominant MEL-B with a purity of about 95% and their molecular weight range is 500 to 850. MAPK inhibitors were purchased from Tocris (#1879; Minneapolis, MN, USA) and used at concentrations selected with reference to previous studies (100 nM SP600125 for JNK, 10 µM PD98059 for ERK, and 5 µM SB203580 for p38) [16 (link)25 (link)].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!