anti-vimentin antibody (D21H3) XP rabbit monoclonal antibody (catalog #5741), anti–cathepsin D (CTSD) antibody (catalog #2284), cleaved caspase-3 (Asp175) (5A1E) rabbit monoclonal antibody (catalog #9664), and anti–cluster of differentiation 68 (CD68) (D4B9C) rabbit monoclonal antibody (catalog # 76437S) were obtained from Cell Signaling Technology. Anti-CD31 rabbit polyclonal antibody (catalog #ab28364) and anti-vimentin antibody (catalog#ab8979) were purchased from Abcam. Anti–α-smooth muscle actin (SMA) antibody was obtained from Novus Biologicals. Antifibrinogen β-chain (FGB) (catalog #HPA001900) and anti-PLG (catalog #HPA048823) antibodies were purchased from Sigma-Aldrich. Anti-CD34 (catalog #MCA547GT) monoclonal antibodies were obtained from Bio-Rad Laboratories. Anti–apolipoprotein E1 (APOE) (catalog #66830-l-lG) and anti-serum amyloid P component (APCS) (catalog #20773-1-AP) antibodies were purchased from Proteintech. Production of anti-goat PLG rabbit polyclonal antibodies were outsourced to SCRUM. The SYPRO Ruby Protein Gel Stain kit and Bio-Safe Coomassie blue stain solution were purchased from Thermo Fisher Scientific and Bio-Rad Laboratories, respectively. Preimplanted Freestyle aortic root and Magna EASE bioprostheses were purchased from Medtronic and Edwards Lifesciences, respectively.
Anti cd34
Manufactured by Bio-Rad
Anti-CD34 is a laboratory reagent used in flow cytometry and cell sorting applications. It is a monoclonal antibody that specifically binds to the CD34 antigen, which is expressed on the surface of hematopoietic stem and progenitor cells. The core function of Anti-CD34 is to enable the identification and isolation of these cell populations from complex biological samples.
Automatically generated - may contain errors
2 protocols using anti cd34
1
Comprehensive Antibody Panel for Cardiovascular Research
2
Isolation and Purification of Mouse Hair Follicle Stem Cells
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Adult C3H/HeN mouse whole skin epithelial cells were isolated from dorsal skin areas by trypsin treatment, as previously reported22 (link), then single cell suspensions in phosphate buffer were exposed to antibodies directly coupled with fluorochrome for 30 min on ice. Antibodies used for FACS analysis were anti-α6 integrin (CD49f.) or rat IgG2a isotype (control) directly coupled with PE (R&D Systems, Inc.), and anti-CD34 (Bio-Rad; formerly AbD Serotec, Hercules, CA) or rat IgG2a isotype (control) (eBioscience, San Diego, CA) coupled with FITC. After washing twice with phosphate buffer, cell purification was performed using a FACSAria system equipped with the FACS DiVa software package (BD Biosciences, San Jose, CA). HFSCs were gated for single events, then sorted according to the expression of CD49f. and CD34. The purity of sorted cells was determined by post-sort FACS analysis and typically exceeded 95%.
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