Mr 400

Manufactured by Agilent Technologies

Sourced in United States

The MR-400 is a compact and versatile laboratory equipment designed for a range of analytical applications. It functions as a magnetic resonance spectrometer, providing users with the ability to perform nuclear magnetic resonance (NMR) measurements and analyses. The core function of the MR-400 is to generate and detect radio frequency signals, which interact with the nuclear spins of samples, enabling the collection of valuable data for various scientific and research purposes.

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Lab products found in correlation

Direct drive 400, by Agilent Technologies (2 mentions) Avance 400, by Agilent Technologies (2 mentions) Vnmrs 500, by Agilent Technologies (2 mentions) Vnmrs 600, by Agilent Technologies (2 mentions) 2 2 azobisisobutyronitrile, by Merck Group (2 mentions) Ethyl methacrylate, by Thermo Fisher Scientific (2 mentions) 1515 hplc instrument, by Waters Corporation (2 mentions) Methyl 3 mercaptopropionate, by Thermo Fisher Scientific (2 mentions) Trifluoroacetic acid tfa and solvents, by Thermo Fisher Scientific (2 mentions) Uv 2550, by Shimadzu (2 mentions) Chromaster hplc system, by Hitachi (2 mentions) Sephadex lh 20 gel, by Cytiva (2 mentions) Cm120, by Philips (1 mentions) Ltq orbitrap xl, by Thermo Fisher Scientific (1 mentions) Avance neo 500, by Bruker (1 mentions) Nano z, by Malvern Panalytical (1 mentions) Orbitrap elite, by Thermo Fisher Scientific (1 mentions) Nicolet is10, by Thermo Fisher Scientific (1 mentions) Spectrum bx ft ir spectrometer, by PerkinElmer (1 mentions) Inova 500, by Agilent Technologies (1 mentions)

Spelling variants of this product

400-MR spectrometer (42 citations) Agilent 400-MR NMR spectrometer (14 citations) 400-MR DD2 (12 citations) MR 400 MHz spectrometer (10 citations) 400-MR-NMR spectrometer (7 citations) 400-MR DDR2 spectrometer (3 citations) VNMRS 400-MR (2 citations) Agilent 400-MR NMR (2 citations) 400-MR DD2 spectrometer (2 citations) MR 400 NMR spectrophotometer (2 citations)

17 protocols using mr 400

Detailed Characterization of Chemical Compounds

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Chemicals and reagents were used
as obtained from Sigma-Aldrich
or Acros without further purification. ¹H NMR spectra were
recorded on a Varian MR400 (at 400 MHz) at ambient temperature. The
splitting patterns are designated as follows: s (singlet); d (doublet);
dd (double doublet); t (triplet); q (quartet); m (multiplet), and
br (broad). ¹³C NMR spectra were recorded on a Varian MR400
(100.6 MHz) at ambient temperature. Chemical shifts are denoted in
δ (ppm), referenced to the residual protic solvent peak. Coupling
constants J are denoted in Hz. Masses were recorded with a Thermo
scientific LTQ Orbitrap XL mass spectrometer. Silicycle Siliaflash
P60, 40–63 m, (230–400 mesh) was used for column chromatography.
Irradiations were performed with Spectroline ENB-280C/FE UV lamps
(8 W). For analysis by cryo-transmission electron microscopy (cryo-TEM),
the turbid solution (2.5 μL) was placed on a glow-discharge
holy carbon-coated grid (Quantifoil 3.5/1, QUANTIFOIL Micro Tools
GmbH, Großlöbichau, Germany). After blotting, the grid
was rapidly frozen in liquid ethane (Vitrobot, FEI, Eindhoven, The
Netherlands) and stored in liquid nitrogen until measurements. Grids
were observed in a Gatan model 626 cryo-stage in a Philips CM120 or
Tecnai T20 cryo-electron microscope operating at 120 or 200 KeV. Images
were recorded under low-dose conditions on a slow-scan CCD camera.

Meng J., Zhang Y., Pan L, & Chen J. (2022). Dynamic Control of Self-Assembly of Amphiphilic Conjugated Alkenes in Water by Reactions. ACS Omega, 7(5), 4677-4682.

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Solvent Purification and NMR Spectroscopy

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CH₂Cl₂ was dried by distillation over CaH₂, and n-hexane by distillation over Na, benzophenone and tetraglyme. For NMR, deuterated solvents were dried by adding 3 Å molecular sieves to freshly opened bottles. All dry solvents were stored over 3 Å molecular sieves in a glovebox. Pyridine was redistilled prior to use. All other chemicals were used without further purification. For all synthesis performed in a glovebox, glassware had been dried at 150 °C in an oven, or in vacuo, at least overnight. NMR spectra were recorded on a Bruker Avance Neo 500 MHz spectrometer equipped with a TXO cryogenic probe, or an Agilent MR-400 equipped with an OneNMR probe. Chemical shifts are reported on the δ scale (ppm), with the residual solvent signal as an internal reference; CD₂Cl₂ (δ_H 5.32, δ_C 53.84), CDCl₃ (δ_H 7.26, δ_C 77.16). Nitromethane (δ_N 0.0 ppm) was used as an external standard for ¹⁵N. To assign the ¹H NMR resonances chemical shift (δ), multiplicity, coupling constants (J Hz) and number of hydrogens were considered. 2D spectra (¹H,¹⁵N HMBC, ¹H,¹³C HSQC, ¹H,¹³C HMBC, TOCSY, and COSY) also aided correct assignment. Multiplicities are denoted as s (singlet), d (doublet), t (triplet), q (quartet), h (heptet), and m (multiplet). MestReNova 12.0.2. was used to process the NMR spectra.

von der Heiden D., Németh F.B., Andreasson M., Sethio D., Pápai I, & Erdelyi M. (2021). Are bis(pyridine)iodine(i) complexes applicable for asymmetric halogenation?. Organic & Biomolecular Chemistry, 19(38), 8307-8323.

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Nanogel Composition and pH-Dependent Size

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Nanogel composition was estimated using 1H Nuclear Magnetic Resonance (NMR) Spectroscopy. Spectra were obtained using a 400 MHz NMR (Varian Direct Drive 400 or Agilent MR 400) at 25°C. To determine composition, lyophilized nanogels were diluted in D₂O or DMSO-d6 at 10 – 15 mg/mL and disulfide crosslinks were degraded via addition of tris(2-carboxylethyl)phosphine to a final concentration of 10mM for 24 hours prior to acquisition. Spectra were analyzed on MestReNova 10.0 software.
Nanoparticle hydrodynamic diameters were measured using a Malvern ZetaSizer NanoZS with a MPT-2 multipurpose titrator. Nanogel samples were suspended at 0.5 mg/mL in 1x PBS and adjusted to pH 4.0. Hydrodynamic diameter was measured in triplicate at the initial pH and in increments of 0.3 up to pH 8.5. The titrator adjusted pH using 0.1 N NaOH, 0.1 N HCl, or 1 N HCl as needed. Each measurement was the average of a minimum of 12 tens second acquisitions.

Spencer D.S., Shodeinde A.B., Beckman D.W., Luu B.C., Hodges H.R, & Peppas N.A. (2020). Biodegradable Cationic Nanogels with Tunable Size, Swelling and pKa for Drug Delivery. International journal of pharmaceutics, 588, 119691.

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Synthesis and Characterization of Novel Heterocyclic Compounds

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All common reagents were purchased from commercial suppliers and were used as received. The melting points were uncorrected. ¹H (400 MHz), ¹³C (101 MHz) and ¹⁹F (376 MHz) NMR spectra were recorded on Bruker Avance 400 and Agilent MR-400 spectrometers in DMSO-d₆ and C₆D₆. Chemical shifts were measured relative to residual solvent signals and referenced in parts per million to tetramethylsilane (TMS). High-resolution mass spectra (HRMS) were recorded on an OrbitrapElite (Thermo Scientific) mass spectrometer with electrospray ionization (ESI) and an orbital trap. To inject solutions with a concentration of 0.1 to 9 mg ml⁻¹ (in 1% formic acid in acetonitrile), direct injection into the ion source using a syringe pump (0.3 ml min⁻¹) was used. The spray voltage was ±3.5 kV, and the temperature of the capillary was 275°C.
Starting compounds 1 and 2a-i were synthesized according to the methods described in [13 (link)].
CCDC 2052348 and 2052655 contain electronic supplementary material, crystallographic data for this study. These data can be obtained free of charge via www.ccdc.cam.ac.uk/data_request/cif, or by emailing data_request@ccdc.cam.ac.uk, or by contacting The Cambridge Crystallographic Data Centre, 12 Union Road, Cambridge CB2 1EZ, UK; fax: +44 1223 336033.

Kukushkin M.E., Kondratieva A.A., Karpov N.A., Shybanov D.E., Tafeenko V.A., Roznyatovsky V.A., Grishin Y.K., Moiseeva A.A., Zyk N.V, & Beloglazkina E.K. (2022). [3+2]-Cycloaddition of azomethine ylides to 5-methylidene-3-aryl-2-сhalcogen-imidazolones: access to dispiro indolinone-pyrrolidine-imidazolones. Royal Society Open Science, 9(3), 211967.

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Characterization of Lyophilized Polymer Nanoparticles

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Lyophilized crosslinked polymer nanoparticles were characterized with Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) Spectroscopy (Thermo Scientific Nicolet iS10) with a germanium crystal. Background spectra were collected immediately before each sample and used for background subtraction. In all cases, spectra were averaged over 64 scans.
Nanogel composition was estimated using ¹H nuclear magnetic resonance (NMR) Spectroscopy. Spectra were obtained using a 400 MHz NMR (Varian Direct Drive 400 or Agilent MR 400) at 25 ºC. To determine composition, dried nanogels were diluted in D₂O at 10–15 mg/mL and degraded via addition of tris(2-carboxylethyl)phosphine to a final concentration of 10 mM for 24 h prior to acquisition. Spectra were analyzed on MestReNova 10.0 software via integration of DEAEMA (δ 1.22, 6H), tBMA (δ 1.31, 9H), and PEGMA2k (δ 3.55 ppm, 176H).

Spencer D.S., Luu B.C., Beckman D.W, & Peppas N.A. (2018). Control of Cationic Nanogel PEGylation in Heterogeneous ARGET ATRP Emulsion Polymerization with PEG Macromonomers. Journal of polymer science. Part A, Polymer chemistry, 56(14), 1536-1544.

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Characterization of Organic Compounds by NMR and MS

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All chemicals were purchased from commercial suppliers and were used as received unless otherwise stated. Proton Nuclear Magnetic Resonance NMR (¹H NMR) spectra and carbon nuclear magnetic resonance (¹³C NMR) spectra were recorded on a Varian Unity Plus 400, Varian MR400, Varian vnmrs 500, Varian Inova 500, Varian Mercury 500, and Varian vnmrs 700 spectrometers. Chemical shifts for protons are reported in parts per million and are references to the NMR solvent peak (CDCl₃: δ 7.26). Chemical shifts for carbons are reported in parts per million and are referenced to the carbon resonances of the NMR solvent (CDCl₃: δ 77.23). Data are represented as follows: chemical shift, multiplicity (br = broad, s = singlet, d = doublet, t = triplet, q = quartet, dq = doublet of quartet, ddq = doublet of doublet of quartet, p = pentet, dd = doublet of doublet, ddd = doublet of doublet of doublet, hept = heptet, m = multiplet), coupling constants in Hertz (Hz) and integration. Mass spectroscopic (MS) data was recorded at the Mass Spectrometry Facility at the Department of Chemistry of the University of Michigan in Ann Arbor, MI on an Agilent Q-TOF HPLC-MS with ESI high resolution mass spectrometer. Infrared (IR) spectra were obtained using either an Avatar 360 FT-IR or Perkin Elmer Spectrum BX FT-IR spectrometer. IR data are represented as frequency of absorption (cm⁻¹).

McAtee C.C., Ellinwood D.C., McAtee R.C, & Schindler C.S. (2018). Functionalized Cyclopentanes via Sc(III)-Catalyzed Intramolecular Enolate Alkylation. Tetrahedron, 74(26), 3306-3313.

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Purification of Compounds Using HPLC

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Spectra data were obtained by using a Varian MR-400 and VNMRS-600 instrument (Varian, Palo Alto, CA, USA). Tetramethylsilane (TMS, δ = 0 ppm) was the internal standard of the chemical shift. Silica gel column chromatography (Qingdao Haiyang Chemical Co.), a preparative high-performance liquid chromatography (HPLC), was conducted using a Shimadzu type LC-20AT (Shimadzu, Tokyo, Japan) with an Octadecylsilyl (ODS) column chromatography (C18 reversed-phase silica gel, SP-120-50-ODS-B, Daisogel, Japan), which were were used to purify the compounds.

Ainiwaer P., Li Z., Zang D., Jiang L., Zou G, & Aisa H.A. (2023). Ruta graveolens: Boost Melanogenic Effects and Protection against Oxidative Damage in Melanocytes. Antioxidants, 12(8), 1580.

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Synthesis and Characterization of Polymeric Antimicrobials

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Ethanolamine, 4-amino-1-butanol, di-tert-butyl dicarbonate, triethylamine, methyl 3-mercaptopropionate, and ethyl methacrylate were purchased from Acros Organics. 2,2’-azobisisobutyronitrile (AIBN) and 2-(trimethylsiloxy)ethyl methacrylate were purchased from Sigma-Aldrich Co. LLC. Trifluoroacetic acid (TFA) and solvents were purchased from Thermo Fisher Scientific, Inc. The chemicals were used without further purification, with the exception of methacryloyl chloride, which were distilled before use. ¹H NMR was performed using a Varian MR400 (400 MHz) and analyzed using VNMRJ 3.2 and MestReNova. Gel permeation chromatography (GPC) analysis was performed using a Waters 1515 HPLC instrument equipped with Waters Styragel (7.8 × 300 mm) HR 0.5, HR 1, and HR 4 columns in sequence and detected by a differential refractometer (RI). Mueller Hinton broth (MHB, BD and Company ©) and phosphate buffered saline (PBS, pH=7.4, Gibco®) were prepared according to manufacturer instructions and sterilized prior to use. Human red blood cells (RBCs) (leukocytes reduced adenine saline added) were obtained from the American Red Cross Blood Services Southeastern Michigan Region and used prior to the out date indicated on each unit.

Mortazavian H., Foster L.L., Bhat R., Patel S, & Kuroda K. (2018). Decoupling the functional roles of cationic and hydrophobic groups in the antimicrobial and hemolytic activities of methacrylate random copolymers. Biomacromolecules, 19(11), 4370-4378.

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NMR Characterization of Dendrimers

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NMR experiments
were performed on Varian VNMRS 500 and Varian MR400 instruments. ¹H NMR spectra were obtained used 10 s preacquisition delays
and a total of 64 scans. All sample solutions were set to a dendrimer
concentration of 5 mg/mL in deuterium oxide.

van Dongen M.A., Rattan R., Silpe J., Dougherty C., Michmerhuizen N.L., Van Winkle M., Huang B., Choi S.K., Sinniah K., Orr B.G, & Banaszak Holl M.M. (2014). Poly(amidoamine) Dendrimer–Methotrexate Conjugates: The Mechanism of Interaction with Folate Binding Protein. Molecular Pharmaceutics, 11(11), 4049-4058.

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Synthesis and Characterization of Methyl 1-Naphthoate

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Reagents and solvents were purchased from commercial suppliers and have not been purified. Melting points were determined in a Quimis Q340.23 apparatus and are uncorrected. ¹H-NMR and ¹³C-NMR spectra were recorded on Bruker AC-200, Bruker DRX-300 and Varian MR-400 (coupling constant (J) values were given in Hertz). Infrared spectra (IR) were carried out in the spectrophotometer apparatus Fourier transform IR Nicolet 6700 FT-IR using tablets of potassium bromide (KBr). Purity of the final product was determined by high-performance liquid chromatography (HPLC) on Shimadzu LC-20AD with Kromasil 100–5 C18 column (4.6 mm × 250 mm), and Detector SPD-M20A (diode array). Analyte quantification was performed using a standardized wavelength, 254 nm, and acetonitrile and water 60% were used as the mobile phase.
Synthetic methodologies used to prepare methyl 1-naphthoate have been carefully described in previously published studies [15 (link),16 (link)]. Moreover, 2-naphthohydrazide was prepared as previously described by Cordeiro et al. [8 (link),9 (link)]. All the spectroscopical data can be accessed in the Supplementary Material.

da Costa Salomé D., de Freitas R.H., Fraga C.A, & Fernandes P.D. (2022). Novel Regioisomeric Analogues of Naphthyl-N-Acylhydrazone Derivatives and Their Anti-Inflammatory Effects. International Journal of Molecular Sciences, 23(21), 13562.

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