Chitin

Manufactured by Sinopharm

Sourced in China

Chitin is a naturally occurring polysaccharide that is a core structural component found in the exoskeletons of crustaceans, such as shrimp and crab, as well as the cell walls of fungi. It is a versatile material with a range of applications in various industries.

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Lab products found in correlation

5 protocols using chitin

Graphite-Based Composite Synthesis

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Graphite powder (granularity ≤30 μm) was purchased from the Sinopharm Chemical Reagent Co., Ltd., with high chemical purity. KMnO₄, FeCl₃·6H₂O, FeSO₄·7H₂O, H₂SO₄ (98%), H₂O₂ (30%), and NH₃·H₂O of analytical grade were purchased from the Laiyang Institute of Chemical Reagents. chitosan with 85% deacetylation was obtained from the Sinopharm Chemical Reagent Co., Ltd. The chemical structures of chitin and chitosan are shown in Supplementary Fig. S5. Glutaraldehyde (50%) was used as the cross-linking agent, which was purchased from the Tianjin Damao Chemical Reagent Co., Ltd. Potassium dichromate (K₂Cr₂O₇) was provided by the Sinopharm Chemical Reagent Co., Ltd. All other reagents used were analytical grade without further purification. Deionized (DI) water was produced using an ultra-pure water purifier system, with a resistivity of 18.2 MΩ·cm⁻¹.

Zhang B., Hu R., Sun D., Wu T, & Li Y. (2018). Fabrication of chitosan/magnetite-graphene oxide composites as a novel bioadsorbent for adsorption and detoxification of Cr(VI) from aqueous solution. Scientific Reports, 8, 15397.

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Synthesis of Natural Rubber Latex Foams

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The Dunlop method was used to prepare the NRLFs. High ammonia natural rubber latex (HA-NRL) was supplied by Yunnan Natural Rubber Industry Group Jingyang Co., Ltd (Yunnan, China). The chemicals (potassium hydroxide, 2-mercaptobenzimidazole, 2-mercaptobenzothiazole, sulfur, potassium oleate, ammonium sulfate, zinc oxide, sodium fluorosilicone), Luria-Bertani solid medium (deionized water, tryptone, sodium chloride, yeast extract, agar) and chitin were supplied by Sinopharm Chemical Reagent Co., Ltd. (China). E. coli BL21 were supplied by Qincheng Biotechnology Co., Ltd. (Shanghai, China). The names, concentrations and proportions of the chemicals used in the synthesis experiment are shown in Table 1.

Zhang N, & Cao H. (2020). Enhancement of the Antibacterial Activity of Natural Rubber Latex Foam by Blending It with Chitin. Materials, 13(5), 1039.

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Chitin-Based Sulfation Protocol

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Chitin was purchased from Sinopharm Chemical Reagent Co., and its degree of deacetylation was 10%. The other reagents, such as chloroform, sodium azide, triphenylphosphine (Ph₃P), 4-toluene sulfonyl chloride, 1,3-propanesulfonate, sulfur trioxide pyridine, N-Methyl pyrrolidone (NMP) and dimethyl sulfoxide (DMSO), etc., were provided by Sinopharm Chemical Reagent Co., Ltd., Shanghai, China.

Luan F., Xu Z., Wang K., Qi X, & Guo Z. (2022). Synthesis of Water-Soluble Sulfonated Chitin Derivatives for Potential Antioxidant and Antifungal Activity. Marine Drugs, 20(11), 668.

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Synthesis of Chitin-based Composites

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Chitin; polyvinylidene fluoride (PVDF), KOH, NaOH, HCl and N,N-dimethylacetamide (DMAC) were purchased form Sinopharm Chemical Reagent Co., Ltd. Conductive carbon black was obtained from Tianjin Jindadi Chemical Co., Ltd. All chemicals are analytical reagents.

Li P., Feng T., Song Z., Tan Y, & Luo W. (2020). Chitin derived biochar for efficient capacitive deionization performance. RSC Advances, 10(50), 30077-30086.

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Chitin Oligosaccharide Synthesis and Characterization

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A One Step Cloning Kit and Taq polymerase were purchased from Vazyme (Nanjing, China). High-fidelity DNA polymerase was obtained from Toyobo (Osaka, Japan). K-ACET was purchased from Megazyme (Bray, Ireland). A Bacteria DNA Kit and a Plasmid Extraction Kit were purchased from Tiangen (Beijing, China). A Gel Extraction Kit was purchased from Omega (Guangzhou, China). Chitin was purchased from Sinopharm Group (Shanghai, China). Ni-NTA Superflow was obtained from Qiagen (Duesseldorf, Germany). N-acetyl glucosamine (GlcNAc), diacetylchitobiose ((GlcNAc)₂), triacetylchitotriose ((GlcNAc)₃), tetraacetylchitotetraose ((GlcNAc)₄), acetylchitopentaose ((GlcNAc)₅), and acetylchitohexaose ((GlcNAc)₆) were purchased from Qingdao Bozhihuili Biotechnology (Qingdao, China). Other chemical reagents used in this study were of analytical grade unless specifically indicated. Colloidal Chitin and carboxymethyl Chitin were prepared according to previously reported methods [50 (link),51 (link)].

Yin L., Wang Q., Sun J, & Mao X. (2022). Expression and Molecular Modification of Chitin Deacetylase from Streptomyces bacillaris. Molecules, 28(1), 113.

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