Hyclone dulbecco s modified eagle medium ham s f12 dmem f12 1 1

Hyclone™ Dulbecco’s Modified Eagle Medium/Ham’s F12 (DMEM/F12 1:1) and Gibco™ fetal bovine serum (FBS) were purchased from ThermoFisher Scientific (Waltham, MA), Rosiglitazone was purchased from AK Scientific (Union City, CA). All other reagents used in the medium were purchased from Sigma-Aldrich (St. Louis, MO). Fucoxanthin (> 95% fucoxanthin, FN), bovine serum albumin (BSA), glycerol standard solution, and taurocholic acid were purchased from Sigma-Aldrich. Glycerol Reagent was purchased from Zenbio (The Triangle, NC). Isoproterenol was purchased from Cayman Chemicals (Ann Arbor, MI). An organic extract of the fucoxanthin plant containing 5wt% fucoxanthin (FEX) was provided by PLT Health Solutions, (Morristown, NJ). The purified alcohol of fucoxanthin, fucoxanthinol (FOL), was purchased from Wako Pure Chemical Industries (Osaka, Japan, 94% fucoxanthinol) and Sigma-Aldrich (≥ 97% fucoxanthinol). Cholesterol esterase was purchased from Worthington Biochemical Corporation (Lakewood, NJ). Protease and phosphatase were purchased from Cell Signaling Technology (Danvers, MA), RIPA buffer from Sigma, TGX protein gels from BIO-RAD (Hercules, CA).

Hyclone™ Dulbecco’s Modified Eagle Medium/Ham’s F12 (DMEM/F12 1:1) and Gibco™ fetal bovine serum (FBS) were purchased from ThermoFisher Scientific (Waltham, MA), Rosiglitazone was purchased from AK Scientific (Union City, CA). All other reagents used in the medium were purchased from Sigma-Aldrich (St. Louis, MO). Naringenin (purity ≥ 98%) was purchased from Cayman Chemicals (Ann Arbor, MI). Protease and phosphatase were purchased from Cell Signaling Technology (Danvers, MA), RIPA buffer from Sigma, TGX protein gels from BIO-RAD (Hercules, CA). Seahorse Assay Medium and sodium pyruvate were purchased from Agilent (Santa Clara, CA). carbonyl cyanide-4-(trifluoromethoxy) phenyhydrazone (FCCP), Oligomycin, and Antimycin A were purchased from Sigma-Aldrich.