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Proteasome glo cell based assay kit

Manufactured by Promega
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The Proteasome-Glo™ Cell-Based Assay Kit is a luminescent assay that measures proteasome activity in live cells. The kit provides the necessary reagents to quantify chymotrypsin-like, trypsin-like, and caspase-like proteasome activities.

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Proteasome-GloTM Chymotrypsin-like Cell-Based Assays kit Proteasome-Glo assay Proteasome-Glo kit Proteasome-Glo

2 protocols using proteasome glo cell based assay kit

1

Proteasome Enzymatic Activities Assay

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The chymotrypsin-like, trypsin-like, and caspase-like activities of proteasome were measured as described elsewhere [49 (link)] using a Proteasome-Glo™ Cell-Based Assay Kit (Promega). Background non-specific peptidase activities were determined by adding proteasome inhibitors: bortezomib (0.1 μg/mL for chymotrypsin-like activity; Calbiochem) or AdaAhx3L3VS (30 μg/mL for trypsin-like and caspase-like activities; Calbiochem). The proteasome activities were calculated by subtracting the values with the inhibitors (non-specific peptidase) from the values without the inhibitors (total peptidase).
Tokuda E., Brännström T., Andersen P.M, & Marklund S.L. (2016). Low autophagy capacity implicated in motor system vulnerability to mutant superoxide dismutase. Acta Neuropathologica Communications, 4, 6.
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2

Proteasome Activity Assay in Cells

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Proteasome activities in cultured cells were measured with Proteasome-Glo cell–based assay kit (Promega, G8532). Chymotryptic-like, tryptic-like, and caspase-like activities were measured separately following the company’s recommended protocols.
Tao P., Wang S., Ozen S., Lee P.Y., Zhang J., Wang J., Han H., Yang Z., Fang R., Tsai W.L., Yang H., Sag E., Topaloglu R., Aksentijevich I., Yu X, & Zhou Q. (2021). Deubiquitination of proteasome subunits by OTULIN regulates type I IFN production. Science Advances, 7(47), eabi6794.
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