Alexa fluor 594 conjugated secondary antibodies

Manufactured by BioLegend

Alexa Fluor 594-conjugated secondary antibodies are fluorescently labeled antibodies that can be used to detect and visualize primary antibodies in various applications, such as immunofluorescence and flow cytometry. The Alexa Fluor 594 dye provides bright, photostable fluorescence with excitation and emission wavelengths of 590 nm and 617 nm, respectively.

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Lab products found in correlation

Desmin, by Abcam (1 mentions) Wheat germ agglutinin (wga), by Thermo Fisher Scientific (1 mentions) Fibronectin fn, by Abcam (1 mentions) Vimentin vim, by Cell Signaling Technology (1 mentions) Vectastain abc dab kit, by Vector Laboratories (1 mentions)

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Alexa Fluor 594

2 protocols using alexa fluor 594 conjugated secondary antibodies

Muscle Fiber Characterization in Mice

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Mouse muscle samples were frozen in liquid nitrogen‐cooled isopentane in Tissue‐Tek OCT. 10 mm sections were stained with haematoxylin–eosin staining and using the following primary antibodies: Slo1 (1:500, Alomone Labs), WGA (conjugated Alexa Fluor 488, Thermo Scientific, cat. no. W6748), and Desmin (1:1000, Abcam, cat. no. ab32362).
Primary mouse myoblasts were cultured in differentiation medium for 3 days and fixed with 4% paraformaldehyde for 15 min and stained with MYH3 (1:100, Santa Cruz Biotechnology) for myotubes.
For signal detection, Alexa Fluor 594‐conjugated secondary antibodies (1:500, Biolegend, cat. no. 406709) were used. Nuclei were labelled with DAPI.

Xia C., Wang Y., Jiang T., Hu Y., Chen Y., Ma X., Zhang X, & Gao Y. (2023). Slo1 deficiency impaired skeletal muscle regeneration and slow‐twitch fibre formation. Journal of Cachexia, Sarcopenia and Muscle, 14(4), 1737-1752.

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Genetically Engineered Mouse Models for Breast Cancer Research

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The generation of p18^−/−, p18^−/−;Brca1^MGKO (p18^−/−; Brca1^f/f;MMTV-Cre or p18^−/−;Brca1^f/−;MMTV-Cre) and p16^−/−;Brca1^MGKO (p16^−/−;Brca1^f/f;MMTV-Cre or p16^−/−; Brca1^f/−;MMTV-Cre) mice has been described previously [23 (link), 37 (link), 39 (link)]. The Institutional Animal Care and Use Committee at the University of Miami approved all animal procedures. Histopathologic analysis and immunohistochemical analysis (IHC) were performed as described previously [19 (link), 23 (link), 37 (link)]. The primary antibodies used were Ck14 (Thermal Scientific), ERα (Santa Cruz), p-Akt (Ser473), p-4E-bp1 (Thr37/46), vimentin (Vim), E-cadherin (E-cad), p-Fra1 (Ser265) (Cell Signaling), Ki67 and fibronectin (Fn) (Abcam). Immunocomplexes were detected using the Vectastain ABC DAB kit according to the manufacturer’s instructions (Vector Laboratories) or by using Alexa Fluor 488-conjugated or Alexa Fluor 594-conjugated secondary antibodies (Biolegend). The positive results of IHC were quantified by the H score, as previously described [41 (link)].

Wang C., Bai F., Zhang L.H., Scott A., Li E, & Pei X.H. (2018). Estrogen promotes estrogen receptor negative BRCA1-deficient tumor initiation and progression. Breast Cancer Research : BCR, 20, 74.

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