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Organic solvents

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Organic solvents are a class of chemical compounds commonly used in laboratory settings. They are known for their ability to dissolve a wide range of organic materials, making them essential for various analytical and extraction procedures. These solvents are available in different types, each with unique properties and applications.

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Lab products found in correlation

Penicillin, by Thermo Fisher Scientific (2 mentions) Streptomycin, by Thermo Fisher Scientific (2 mentions) Mpeg dspe, by Laysan Bio (2 mentions) Ketek, by Sanofi (2 mentions) Chloroform, by Thermo Fisher Scientific (2 mentions) N decane, by Thermo Fisher Scientific (2 mentions) Phospholipid1 2 diphytanoyl sn glycerol 3 phosphocholine, by Avanti Polar Lipids (2 mentions) Orlistat, by Merck Group (1 mentions) Tlc plate, by Merck Group (1 mentions) L glucose, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Sodium citrate, by Thermo Fisher Scientific (1 mentions) Azocasein, by Merck Group (1 mentions) Trichloroacetic acid tca, by HiMedia (1 mentions) Orcinol, by HiMedia (1 mentions) Elastin congo red, by Merck Group (1 mentions) Myriocin, by Merck Group (1 mentions) Wild type cho, by American Type Culture Collection (1 mentions) Ly b8, by RIKEN BioResource Center (1 mentions) Glutamax suplement, by Thermo Fisher Scientific (1 mentions)

24 protocols using organic solvents

1

Isolated Islet and MIN6 Cell Culture

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All tissue culture media, supplements and trypsin for MIN6 cells and isolated islets were from Thermo Fisher Scientific Australia (Scoresby, VIC, Australia). Insulin RIA kits were from Linco/Millipore (Billerica, MA). DMSO, fatty acid free fraction V BSA, palmitate disodium salt, Orlistat, all neutral lipid standards, TLC plates, and l-glucose, were from Sigma–Aldrich (St. Louis, MO). The BCA (bicinchoninic acid assay) protein assay kit was from Pierce (Rockford, IL). Organic solvents were from Thermo Fisher. 3H-sphinganine was from American Radiolabelled Chemicals (St Louis MO).
Pearson G.L., Mellett N., Chu K.Y., Boslem E., Meikle P.J, & Biden T.J. (2016). A comprehensive lipidomic screen of pancreatic β-cells using mass spectroscopy defines novel features of glucose-stimulated turnover of neutral lipids, sphingolipids and plasmalogens. Molecular Metabolism, 5(6), 404-414.
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2

Analytical Methodology for Carbamazepine

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The CBZ standard was from the National Institute for Food and Drug Control, Beijing, China. The CBZE standard and a reference solution of CBZD were purchased from J&K Scientific (Beijing, China). CBZ tablets (100 × 100 mg tablets) were obtained from Guangdong Huanan Pharmaceutical Group Co., Ltd. (Dongguan, China) for the therapeutic drug monitoring experiment. Sodium chloride injections used for gavage were obtained from Hunan Kelun Pharmaceutical Co., Ltd. (Yueyang, China). Analytical grade silver nitrate (AgNO3), potassium iodide, sodium citrate, and organic solvents were purchased from Thermo Fisher Scientific (Waltham, MA, USA) and used without any purification. Distilled water was obtained using a Smart-DUV (18 MΩ·cm resistivity) filter (Shanghai Hitech Instruments Co. Ltd., Shanghai, China). All other reagents were of analytical grade. Single-sided polished silicon wafers (thickness: 525 ± 25 μm) were from Beijing Topvendor Technology Co., Ltd. (Beijing, China).
Zhu Q., Li X., Li D., Lu F., Zhao Y, & Yuan Y. (2022). A Rapid Therapeutic Drug Monitoring Strategy of Carbamazepine in Serum by Using Coffee-Ring Effect Assisted Surface-Enhanced Raman Spectroscopy. Molecules, 28(1), 128.
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3

Quantifying Protein and Elastin Content

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Orcinol and trichloroacetic
acid (TCA) were procured from HiMedia Laboratories, India. Elastin–Congo
red (ECR) and azocasein were purchased from Sigma Aldrich. All organic
solvents were obtained from Thermo Fisher Scientific, India.
Qais F.A., Ahmad I., Altaf M, & Alotaibi S.H. (2021). Biofabrication of Gold Nanoparticles Using Capsicum annuum Extract and Its Antiquorum Sensing and Antibiofilm Activity against Bacterial Pathogens. ACS Omega, 6(25), 16670-16682.
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4

Serine-SPT Deficient CHO Cell Line

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Wild type CHO (ATCC, Manassas, Virginia, U.S.) and a serine-SPT deficient CHO cell line, known as LY-B8 (link) (RIKEN BioResource Research Center, Koyadai, Japan), were used. Cell culture products; DMEM:F12 (Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12) FBS (Fetal Bovine Serum), penicillin, streptomycin, and GlutaMax suplement were purchased from Thermofisher (Waltham, MA). Organic solvents were from Thermofisher (Waltham, MA). All fluorophores; were purchased from Thermofisher (Waltham, MA). Salts for buffer preparation (KCl, NaCl, CaCl2, HEPES), myriocin, L-α-phosphatidylcholine from chicken egg (PC), sphingomyelin from chicken egg (SM) and D-erythro-sphinganine (sphinganine) were purchased from Sigma-Aldrich (St. Louis, MO, U. S.). All other reagents (salts and Organic solvents) were of analytical grade.
Monasterio B.G., Jiménez-Rojo N., García-Arribas A.B., Riezman H., Goñi F.M, & Alonso A. (2022). Plasma membrane effects of sphingolipid-synthesis inhibition by myriocin in CHO cells: a biophysical and lipidomic study. Scientific Reports, 12, 955.
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5

Cannabidiol Extraction and Purification

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All reagents were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Cannabidiol was purchased from Mile High Labs (Broomfield, CO, USA). Organic solvents were purchased from Fisher Scientific (Hampton, NH, USA).
Perez E., Fernandez J.R., Fitzgerald C., Rouzard K., Tamura M, & Savile C. (2022). In Vitro and Clinical Evaluation of Cannabigerol (CBG) Produced via Yeast Biosynthesis: A Cannabinoid with a Broad Range of Anti-Inflammatory and Skin Health-Boosting Properties. Molecules, 27(2), 491.
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6

Lipid-based Nanoparticle Formulation

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DSPE-PEG2000 is from Laysan Bio, Inc (mPEG-DSPE, MW 2,000). L-a-Phosphatidylcholine is from Sigma-Aldrich (Catalog No. P-2772). F12511 and K-604 were custom synthesized by WuXi AppTec in China. Based on HPLC/MS and NMR profiles, purity of F12511 was 98% and in stereospecificity; purity of K-604 was 98% in chemical purity. Organic solvents are from Fisher Scientific.
De La Torre A.L., Smith C., Granger J., Anderson F.L., Harned T.C., Havrda M.C., Chang C.C, & Chang T.Y. (2021). Facile method to incorporate high-affinity ACAT/SOAT1 inhibitor F12511 into stealth liposome-based nanoparticle and demonstration of its efficacy in blocking cholesteryl ester biosynthesis without overt toxicity in neuronal cell culture. Journal of neuroscience methods, 367, 109437.
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7

Purification and Characterization of Antibiotics

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All reagents were purchased from Sigma-Aldrich (Oakville, Ontario, Canada) unless otherwise specified. Acetyl-coenzyme A (CoA) was purchased from BioShop (Burlington, Ontario, Canada). Organic solvents were purchased from Fisher Scientific (Ottawa, Ontario, Canada). Telithromycin was purified from the pharmaceutical formulation Ketek (400 mg, Sanofi-Aventis US). Briefly, pills were crushed in a mortar and pestle, dissolved in acetonitrile at 40 °C, passed through an Amicon Ultra-15 10 kDa centrifugal filter unit and lyophilized. Linopristin and flopristin were gifts from AstraZeneca.
Pawlowski A.C., Wang W., Koteva K., Barton H.A., McArthur A.G, & Wright G.D. (2016). A diverse intrinsic antibiotic resistome from a cave bacterium. Nature Communications, 7, 13803.
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8

Phytochemical Analysis of Botanical Materials

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Botanical materials were purchased from different sources listed in Table 1. All reagents including compounds in Table 2 were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Organic solvents were purchased from Fisher Scientific (Hampton, NH, USA). All chemicals were analyzed by LC/MS (Agilent 1100; Santa Clara, CA, USA), 1H and 13C NMR (500 MHz and 125 MHz, Bruker, Billerica, MA, USA) for structural identity and confirmed to be >95% pure by analytical HPLC (Agilent 1200; Santa Clara, CA, USA).
Huber K.L., Fernández J.R., Webb C., Rouzard K., Healy J., Tamura M., Stock J.B., Stock M, & Pérez E. (2021). AGSE: A Novel Grape Seed Extract Enriched for PP2A Activating Flavonoids That Combats Oxidative Stress and Promotes Skin Health. Molecules, 26(21), 6351.
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9

Comparative Analysis of E-Cigarette Constituents

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Infasurf® (lot: 112,809,225) was a generous gift from ONY Inc. (Amherst, NY). All organic solvents used in these studies were purchased from Fisher Scientific (Hampton, NH). Purified water used for all the experiments was obtained from an ELGA PURELAB Classic water purifier (High Wycombe, UK) and was used with a resistivity of 18.2 MΩ·cm. Unflavored, as well as berry- and mint-flavored e-cigarettes (all at 2.4% nicotine content) were purchased from Blu (Charlotte, NC). Conventional research cigarettes (1R6F) were purchased from the University of Kentucky Center for Tobacco Products (Lexington, KY). Glass fiber filters used to capture tar were purchased from EMD Millipore (Billerica, MA). Nicotine, isoprene, and acetaldehyde were all purchased from Fisher Scientific.
Przybyla R.J., Wright J., Parthiban R., Nazemidashtarjandi S., Kaya S, & Farnoud A.M. (2017). Electronic cigarette vapor alters the lateral structure but not tensiometric properties of calf lung surfactant. Respiratory Research, 18, 193.
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10

Organic Solvent Preparation for HPLC

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Organic solvents and
HPLC-grade water were purchased from Fisher
Scientific (Ottawa, ON, Canada). All other reagents were purchased
from Sigma-Aldrich Canada (Oakville, ON, Canada) unless stated otherwise.
Lange P.F., Huesgen P.F., Nguyen K, & Overall C.M. (2014). Annotating N Termini for the Human Proteome Project: N Termini and Nα-Acetylation Status Differentiate Stable Cleaved Protein Species from Degradation Remnants in the Human Erythrocyte Proteome. Journal of Proteome Research, 13(4), 2028-2044.
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