Alginic acid sodium salt, calcium chloride (CaCl2) dihydrate, polyethylene glycol sorbitan monooleate (Tween 80), xanthan gum, and chitosan (medium molecular weight, >75% deacetylated) were purchased from Sigma-Aldrich (Milan, Italy). Ozoile (Stable Ozonides) is a formulation obtained from a patented mixing process (EP3900821A1) in which a defined oxygen–ozone mixture is bound to olefinic bonds of fatty acids of a biological olive oil forming stable ozonides. Deionized water (resistivity 18.2 MΩ⋅cm) was produced by a Direct-Q3 UV water purification system (Millipore, Molsheim, France) and used in all preparations.
Direct q 3 uv water purification system
Manufactured by Merck Group
Sourced in Germany, France, United States, United Kingdom, Spain, Japan
The Direct-Q 3 UV water purification system is a compact and reliable lab equipment designed to produce high-purity water. It utilizes a UV lamp to remove microorganisms and a resin-based cartridge to remove impurities, ensuring the delivery of pure, deionized water.
Automatically generated - may contain errors
Lab products found in correlation
Sodium hydroxide (naoh), by Merck Group (7 mentions)
Methanol, by Merck Group (6 mentions)
Acetonitrile, by Merck Group (6 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (4 mentions)
Hydrochloric acid (hcl), by Merck Group (4 mentions)
Sulfuric acid, by Merck Group (4 mentions)
Dichloromethane, by Merck Group (4 mentions)
Acetic acid, by Merck Group (4 mentions)
Ammonium acetate, by Merck Group (4 mentions)
Isopropanol, by Merck Group (3 mentions)
Potassium hydroxide, by Merck Group (3 mentions)
Ammonium formate, by Thermo Fisher Scientific (3 mentions)
Hydrogen peroxide, by Merck Group (3 mentions)
Ethanol, by Merck Group (3 mentions)
Acetone, by Merck Group (3 mentions)
Toluene, by Merck Group (3 mentions)
Lc ms grade methanol, by Honeywell (3 mentions)
Chitosan, by Merck Group (2 mentions)
Alginic acid sodium salt, by Merck Group (2 mentions)
Formic acid, by Biosolve (2 mentions)
69 protocols using direct q 3 uv water purification system
1
Ozone-Infused Olive Oil Protocol
2
Carboxylated Carbon Nanotubes in Hydrogels
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Carboxyl-functionalized multi-walled carbon nanotubes (MWCNT-COOH) with average outer diameter 30 nm and length 1–5 µm (PD30L1-5-COOH, purity > 95%) were obtained from NanoLab (Waltham, MA, USA). These nanotubes were prepared by reflux in concentrated sulfuric/nitric acid and are highly functionalized (2–7 wt% of COOH groups by titration as reported by the manufacturer). The following products were supplied by Sigma-Aldrich (Schnelldorf, Germany) and used without further purification: N-Isopropylacrylamide (NIPAM, 97%, 415324), N,N′-methylenebisacrylamide (MBA, 99%, 146072), and 2-hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone (Irgacure 2959, 98%, 410896). Ultrapure deionized water (resistivity 18.2 MΩ·cm) was produced by a Direct-Q3 UV water purification system (Millipore, Molsheim, France) and used in all preparations.
3
Antioxidant Potential of Tuscia Hazelnut Skins
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Hazelnut skins (Tonda Gentile Romana and Tonda di Giffoni variety) were obtained from a local producer (Bionocciola srl, Carbognano, Viterbo, Italy) after roasting hazelnuts at 150 °C for 24 min. To measure the antioxidant activity, total polyphenol, and total flavonoid contents, 0.5 g of two varieties of Tuscia hazelnut skins were extracted with a solvent consisting of methanol:water (80:20, v/v) in a ratio 1:20. A total of 20 mg of two varieties of Tuscia hazelnuts skins were used for metabolomics analysis. The 1:25 mixture was shaken at room temperature for 2 h, and then centrifuged at 1000× g for 10 min, the supernatant was used for all analyses. All chemicals used were of analytical grade and were purchased from Sigma Aldrich (Milano, Italy). Stock standard solutions of phenolic compounds were prepared by rigorous dissolution of the commercial reagent in methanol. High-purity water from a Direct-Q 3 UV water purification system (Millipore, Burlington, MA, USA) was used for all analyses.
4
Synthesis of Metal-Doped Nanoparticles
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Silver nitrate (AgNO3, ≥99.8%) was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). NaBH4 (≥98.0%), ZnSO4·7H2O (≥99.5%), Ca(NO3)2·4H2O (≥99.0%), and FeSO4·7H2O (≥99.0%) were acquired from Xilong Scientific Co., Ltd. (Shantou, China). PVP (Mw ≈ 40,000 g/mol) was purchased from Sigma-Aldrich (St. Louis, MO, USA). CoSO4·7H2O (≥99.0%), CuSO4·5H2O (≥99.0%), NiSO4·6H2O (≥99.0%), NaF (≥98.0%), CdSO4·8/3H2O (≥99.0%), and PbCl2 (≥99.0%) were obtained from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Ultra-pure water was obtained by a Direct-Q 3 UV water purification system (Millipore, Burlington, MA, USA) and used throughout. All chemicals were used as received without further purification. All metal ion stock solutions were prepared in ultra-pure water.
5
Synthesis of Iron-Based Nanostructures
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Silver nitrate (AgNO3, ≥99.8%), acetone (≥99.5%), NaCl (99.5%), NaI (99.5%), and absolute ethyl alcohol (≥99.5%) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Polyvinylpyrrolidone (PVP) (Mw ≈ 40,000 g/mol) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Ferric chloride hexahydrate (FeCl3·6H2O, ≥99.5%) was obtained from Kemiou Chemical Reagent Co., Ltd. (Tianjin, China). Ferric tribromide (FeBr3, 98%) was obtained from Macklin Biochemical Co., Ltd. (Shanghai, China). Ethylene glycol (EG, ≥99.5%) was acquired from Xilong Scientific Co., Ltd. (Shantou, China). Ultra-pure water obtained by a Direct-Q 3 UV water purification system (Millipore, Burlington, MA, USA) and used throughout. All chemicals were used as received without further purification.
6
Graphene Nanoplatelets for Biomaterial Synthesis
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Graphene nanoplatelets were purchased from XG Sciences (Lansing, MI, USA) and they were of grade C750, consisting of aggregates of platelets with a diameter of less than 2 µm, thickness of a few nm, and average surface area of 750 m2/g according to the manufacturer datasheet. Two-component polydimethylsiloxane (PDMS, Sylgard 184) was obtained from Dow Corning (Midland, MI, USA). Double-stranded DNA (product number 74782, purity ≤1% protein, A260/280 nm ≥ 1.5) was purchased from Sigma-Aldrich (Milan, Italy) and used as received. Hydroxyl-terminated polydimethylsiloxane (PDMS–OH, average Mn ~550) was obtained from Sigma-Aldrich (Milan, Italy). Sterile deionized water (resistivity 18.2 MΩ·cm) was produced by a Direct-Q3 UV water purification system (Millipore, Molsheim, France) and used to prepare DNA solutions and DNA/graphene dispersions.
7
HPLC Buffer Preparation Protocol
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Dimethyl sulfoxide (DMSO) (purity: ≥99.7%) and acetonitrile (gradient grade for liquid chromatography, LiChrosolv®), sodium phosphate dibasic dehydrate and sodium phosphate monobasic monohydrate were purchased from Sigma-Aldrich (Steinheim, Germany). Ultrapure water, obtained with the Millipore Direct-Q 3 UV Water Purification System (Millipore Corporation, Bedford, MA, USA), was utilized for purification of the water used for preparation of the buffer mobile phase.
8
Determination of Biogenic Amines in Solutions
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Standards of BAs with a purity of 99% were obtained from Sigma-Aldrich (St. Louis, MO, USA). Solutions of BAs were prepared with a dilution buffer called sodium cycle, composed of 1.5 mM NaN3, 197 mM NaCl, and 73 mM citric acid in Milli-Q water. Furthermore, we used citric acid, sodium citrate, isopropanol, potassium hydroxide, potassium bromide, hydrochloric acid, and ninhydrin, all purchased from Sigma-Aldrich. Methyl cellosolve was purchased from Ingos (Prague, Czech Republic), as well as tin chloride. All buffer solutions were prepared with deionized water obtained using reverse osmosis Aqual 25 (Aqual s.r.o., Brno, Czech Republic). The deionized water was further purified by using a Direct-Q 3 UV Water Purification System equipped with an UV lamp (Millipore, Billerica, MA, USA). The resistance of Milli-Q H2O was 18 MΩ·cm−1. The pH was measured using a pH meter WTW inoLab (Weilheim, Germany).
9
EGaIn and Silicone Oil Preparation
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EGaIn (Gallium 75.5%, Indium 24.5%, >99.99%) and silicone oil (for oil bath, 100 mPa·s, product no. 85409) were purchased from Sigma Aldrich (St. Louis, MO, USA). DMSO (>99.5%), benzene (>99.5%), and n-hexane (>95%) were purchased from Daejung Chemicals & Metals (Siheung, Korea). Ethanol (>95.0%), and pH 4, 7, and 10 buffer solutions were obtained from Samchun Chemicals (Seoul, Korea). DI water (≥18 MΩ cm) was produced using a Millipore/Direct-Q3UV Water purification system (Burlington, MA, USA).
10
Serum Metabolite Extraction Protocol
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Deionised water for mobile phases was purified in-house by DIRECT-Q 3UV water purification system (Millipore, Watford, UK). Methanol, ethanol, isopropanol (Sigma–Aldrich, Poole, UK), formic acid (Biosolve, Valkenswaard, The Netherlands) and ammonium formate (Fisher Scientific, Schwerte, Germany) were LC/MS grade. Captiva EMR-lipid 96-well plates (Agilent, Cheadle, UK) were used for metabolite extractions on serum samples.
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