HConFs were inoculated into 96-well plates (4×103cells/well) and pretreated with different concentrations of TGF-β2 for 24h before S58 (20nM) addition for 24h or longer, and then cultured in 100μL of fresh complete medium for 48h. 10μl of CCK-8 reagent (CCK-8, Dojindo, Molecular Technologies) was added per well. After incubation for 1h at 37°C, The absorbance values of each well after incubation was measured by a microplate reader (Multiskan Go Multimode Reader, Thermo Scientific) and statistically analyzed.
Multiskan go multimode reader
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Multiskan Go Multimode Reader is a versatile instrument designed for diverse absorbance and fluorescence-based assays. It can perform measurements across a wide range of microplate formats with high precision and accuracy.
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Lab products found in correlation
4 protocols using multiskan go multimode reader
1
TGF-β2 Modulation of Cell Viability
2
Metformin Enhances TGF-β2 Effects
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The cells suspension was inoculated into 96-well plates (4 × 103 cells/well) and preconditioned by TGF-β2 with appear or without appear metformin (2 mM) treatment for 24, 48 and 72 h. Then, HConFs were cultured in 100 μl fresh complete medium (48 h) and then added 10 μl CCK-8 reagent/well. After incubating for 1 h at 37°C, the absorbance values were measured with a microplate reader (Multiskan Go Multimode Reader, Thermo Scientific).
3
Cell Proliferation and Clonogenic Assay
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Cell proliferation was measured with the Cell Counting Kit-8 (Dojindo, Japan). Cells were seeded into 96-well plates with a density of 2000 cells each well and 100 ul medium. Cellular proliferation was measured according to the readout at the wavelength of 450 nm. Data were read by a microplate reader (Multiskan Go Multimode Reader; Thermo Scientific). To determine their clonogenic ability, MDA-MB-231 and MCF-7 cells were trypsinized and seeded in 6-well plates. The medium was changed every three days, and cells were cultured for up to 14 day until colonies were clearly visible. At the endpoint, cells were washed twice with PBS, fixed with 4% paraformaldehyde, stained with crystal violet (Beyotime, China) for 30 min, and then colonies with > 50 cells were counted.
4
Viability and Cytotoxicity Assays
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Cells were cultured in 96-well plates with a density of 3,000 cells in each well and 100 uL of RPMI-1640 medium. Cellular viability was measured using the Cell Counting Kit-8 (Dojindo, Kumamoto, Japan) at the wavelength of 450 nm. Data were read by a microplate reader (Multiskan Go Multimode Reader; Thermo Scientific, Massachusetts, USA). For Trypan Blue dye exclusion assay, cells were cultured in 6-well plates with a density of 5-8*10^5 cells in each well and 2 mL of RPMI-1640 medium. A homogeneous cell suspension of 10 uL was transferred to a cell counting plate. To this suspension, 1 uL of 0.4% Trypan Blue dye was added. The suspension was mixed well and left for 5-10 min at room temperature. Then, the cell counting plate was directly inserted into the automatic cell counter (TC20, Bio-Rad, Hercules, CA) to count the number of living cells.
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