Log In Sign up
The largest database of trusted experimental protocols

Anti cd127

Manufactured by BioLegend
Visit Supplier
Sourced in United States

Anti-CD127 is a lab equipment product that is used to detect the presence of the CD127 protein. CD127 is a cell surface marker that is expressed on various immune cells, including T cells and natural killer cells. The anti-CD127 product can be used in flow cytometry and other immunological applications to identify and characterize cells that express this protein.

Automatically generated - may contain errors

Lab products found in correlation

Anti cd4, by BioLegend (9 mentions) Anti cd3, by BioLegend (7 mentions) Anti cd8, by BioLegend (7 mentions) Anti cd45, by BioLegend (5 mentions) Anti cd45ra, by BioLegend (4 mentions) Anti cd226, by BioLegend (3 mentions) Anti tigit, by Thermo Fisher Scientific (3 mentions) Anti cd11c, by BioLegend (3 mentions) Anti cd44, by BioLegend (3 mentions) Anti cd11b, by BioLegend (3 mentions) Anti ccr7, by BioLegend (3 mentions) Anti pd 1, by BioLegend (2 mentions) Anti foxp3 percp cy5, by Thermo Fisher Scientific (2 mentions) Anti helios, by BioLegend (2 mentions) Foxp3 staining buffer set, by Thermo Fisher Scientific (2 mentions) Lsrfortessa, by BD (2 mentions) Anti thy1, by BD (2 mentions) Lsrii flow cytometer, by BD (2 mentions) Prism 6, by GraphPad (2 mentions) Anti cd86, by BioLegend (2 mentions)

Close spelling variants of this product

APC/Cyanine7 anti-human CD127 (IL-7Rα) Antibody Anti-CD127 PerCP-Cy5.5 Anti–CD127-BV421 (A7R34; Anti-CD127 (clone A7R34, BV421 anti-CD127 Anti-CD127 PE BV650-labeled anti-CD127 Anti-CD127-FITC APC anti-human CD127 Anti-CD127-APC

20 protocols using anti cd127

1

CD4+ T Cell Subpopulation Isolation and Phenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols
CD4+ T cells were enriched by magnetic activated cell sorting (MACS) using 20 µl of anti-CD4 MicroBeads (anti-CD4 MicroBeads, human, Miltenyi Biotec, Germany) and 80 µl MACS buffer (PBS with 0.5% BSA and 2 mM ethylenediaminetetraacetic acid) per 107 cells. CD4+ T cells were stained for surface expression in MACS buffer at a concentration of 2 × 108 cells/ml. Cells were washed and stained with 4′,6-diamidino-2-phenylindole (1:250) and then sorted using the flow cytometer BD FACSAria II (BD Biosciences, Germany) into the following CD4+ T-cell subpopulations: Treg (CD25highCD127low) and non-Treg: TN (CD45RA+ CCR7+), TCM (CD45RA-CCR7+), TEM (CD45RA− CCR7−), and TEMRA (CD45RA+ CCR7−).
Antibodies used for surface staining were anti-CCR7 (GO43H7, 1:50), anti-CD25 (M-A251, 1:100), anti-CD45RA (HI100, 1:50), anti-CD127 (A019D5, 1:100), anti-CD3 (UCHT1, 1:200), anti-CD4 (OKT4, 1:200), anti-PD-1 (EH12.2H7, 1:20), anti-TIGIT (A15153G, 1:20), anti-TIM-3 (F38–2E2, 1:20), and LAG-3 (11C3C65, 1:20) from BioLegend, and anti-KLRB1 (191B8, 1:10), anti-KLRF1 (4A4.D10, 1:50), and anti-KLRG1 (REA261, 1:10, 1:50 since 2018/06 due to more concentrated formulation) from Miltenyi Biotec. See also Supplementary Fig. 5 for the pre-gating strategy.
Truong K.L., Schlickeiser S., Vogt K., Boës D., Stanko K., Appelt C., Streitz M., Grütz G., Stobutzki N., Meisel C., Iwert C., Tomiuk S., Polansky J.K., Pascher A., Babel N., Stervbo U., Sauer I., Gerlach U, & Sawitzki B. (2019). Killer-like receptors and GPR56 progressive expression defines cytokine production of human CD4+ memory T cells. Nature Communications, 10, 2263.
+ Open protocol
+ Expand
2

Comprehensive Phenotyping of Human CD4+ T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Freshly obtained peripheral blood mononuclear cell (PBMC) were stained with anti-CD4 (-FITC from Biolegend, OKT4, USA), anti-CD25 (-PE from Biolegend, BC96, USA), anti-CD127 (-PECY7 from Biolegend, A019D5, USA), anti-CD226 (-APC from Biolegend, 11A8, USA), anti-TIGIT (-APC from eBioscience, MBSA43, USA). Intracellular detection of Foxp3 with anti-Foxp3 (-Percp-Cy5.5 from eBioscience, 236A/E7, USA) and Helios with anti-Helios (-APC from Biolegend, 22F6, USA) was performed on fixed and permeabilized cells via Foxp3 Staining Buffer Set (eBioscience, USA). Cell fluorescence was acquired on BD LSR Fortessa (BD Biosciences, USA) and analyzed with FlowJo software (version 7.6.5; Tree Star). We usually acquired 10, 000 events in FSC. CD4-FITC positive and SSC gates were used to delineate CD4+ cells, then gated with CD25-PE and CD127-PECY7 in these cells, and the acquisition gate was designed on the CD4+CD25highCD127low/− cells.
Yang M., Liu Y., Mo B., Xue Y., Ye C., Jiang Y., Bi X., Liu M., Wu Y., Wang J., Olsen N., Pan Y, & Zheng S.G. (2019). Helios but not CD226, TIGIT and Foxp3 is a Potential Marker for CD4+ Treg Cells in Patients with Rheumatoid Arthritis. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 52(5), 1178-1192.
+ Open protocol
+ Expand
3

Glucose Titration in T Cell Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Splenocytes were isolated and prepared as a single-cell suspension. 3×106 WT and 2B4KO OT-I T cells, respectively, were resuspended in 1.5 mL of complete media (glucose-free RPMI-1640 supplemented with 10% FCS, 2 mM L-glutamine), 100 U/mL penicillin, 100 μg/mL streptomycin, 10 mM HEPES, and 0.5 mM 2-mercaptoethanol) in a 24-well flat-bottomed plate at 37°C in a 5% CO2, humidified atmosphere. A D-glucose solution of 200g/L (Gibco by Life Technologies) was titered into the glucose-free complete media and diluted on a half-log scale. Cells were then stimulated with SIINFEKL N4 peptide at 1 nM for 5 days. After 5 days in culture cells were harvested and stained with anti-CD8 (BD Horizon), anti-Thy1.1 (BD Biosciences or BioLegend), anti-CD244 (eBioSciences), anti-Va2 (BD), anti-CD127 (BioLegend), anti-CD62L (BD), anti-KLRG-1, anti-Vb5 (BD), anti-CD44 (BD), 7AAD (BD) and AnnexinV (BioLegend), anti-IL-2 (BD), anti-IFN-g (BD) as described by the manufacturer. As described above, samples were analyzed on an LSRII flow cytometer (BD Biosciences) and data was analyzed using FlowJo 9 software (Treestar, San Carlos, CA) and Prism 6 software (GraphPad Software Inc.).
Laurie S.J., Liu D., Wagener M.E., Stark P.E., Terhorst C, & Ford M.L. (2018). 2B4 Mediates Inhibition of CD8+ T Cell Responses via Attenuation of Glycolysis and Cell Division. Journal of immunology (Baltimore, Md. : 1950), 201(5), 1536-1548.
+ Open protocol
+ Expand
4

Multiparameter Flow Cytometry Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were washed in FACS medium (PBS containing 2% FBS), and stained with primary antibodies including anti-human CD14-APC (eBioscience, 61D3, 1:100), anti-human CD4-APC (eBioscience, RPA-T4, 1:100), anti-human CD3-FITC (BioLegend, HIT3a, 1:100), anti-human CD8-PE (BD Pharmingen, 555367, 1:100), anti-human CD69-APC (BioLegend, FN50, 1:100), anti-human CD86-PE (BioLegend, IT2.2, 1:100), anti-CD206-APC (eBioscience, 19.2, 1:100), anti-CD11c (Biolegend, Bu15, 1:100), anti-hLAIR1 (eBioscience, NKTA255, 1:100), anti-CD45 (Biolegend, 2D1, 1:100), anti-CD25 (Biolegend, BC96, 1:100), or anti-CD127 (Biolegend, A019D5, 1:100). Flow data were analyzed by Flowjo software. Propidium iodide (PI) staining was used to exclude dead cells in analysis.
Xie J., Gui X., Deng M., Chen H., Chen Y., Liu X., Ku Z., Tan L., Huang R., He Y., Zhang B., Lewis C., Chen K., Xu L., Xu J., Huang T., Liao X.C., Zhang N., An Z, & Zhang C.C. (2022). Blocking LAIR1 signaling in immune cells inhibits tumor development. Frontiers in Immunology, 13, 996026.
+ Open protocol
+ Expand
5

Multiparameter Immune Response Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Single cell suspensions were obtained from PBMCs, and various tissues as described previously. Dead cells were gated out using Live/Dead fixable dead cell stain (Invitrogen). SARS-CoV-2 spike and SF162 peptide pools used for intracellular cytokine staining (ICS) and these were obtained from BEI Resources. Biotinylated MHC class I monomers (KbVL8, sequence VNFNFNGL; DbGP33, sequence KAVYNFATC; DbGP276, sequence SGVENPGGYCL; OVA, sequence SIINFEKL; KbB8R, sequence TSYKFESV) were used for detecting virus-specific CD8 T cells, and were obtained from the NIH tetramer facility at Emory University. Cells were stained with fluorescently-labeled antibodies against CD8α (53–6.7 on PerCP-Cy5.5), CD44 (IM7 on FITC), CD62L (MEL-14 on PE-Cy7), CD127 (A7R34 on Pacific Blue), and tetramers (APC). TNFα (MP6-XT22 on PE-Cy7), IFNγ (XMG1.2 on APC), or Ki67 (SolA15 on PE-Cy7). Fluorescently-labeled antibodies were purchased from BD Pharmingen, except for antiCD127 and anti-CD44 (which were from Biolegend). Flow cytometry samples were acquired with a Becton Dickinson Canto II or an LSRII and analyzed using FlowJo v10 (Treestar).
Sanchez S., Dangi T., Awakoaiye B., Irani N., Fourati S., Richner J, & Penaloza-MacMaster P. (2024). Time-dependent enhancement of mRNA vaccines by 4–1BB costimulation. bioRxiv.
+ Open protocol
+ Expand
6

Comprehensive Phenotyping of Human CD4+ T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Freshly obtained peripheral blood mononuclear cell (PBMC) were stained with anti-CD4 (-FITC from Biolegend, OKT4, USA), anti-CD25 (-PE from Biolegend, BC96, USA), anti-CD127 (-PECY7 from Biolegend, A019D5, USA), anti-CD226 (-APC from Biolegend, 11A8, USA), anti-TIGIT (-APC from eBioscience, MBSA43, USA). Intracellular detection of Foxp3 with anti-Foxp3 (-Percp-Cy5.5 from eBioscience, 236A/E7, USA) and Helios with anti-Helios (-APC from Biolegend, 22F6, USA) was performed on fixed and permeabilized cells via Foxp3 Staining Buffer Set (eBioscience, USA). Cell fluorescence was acquired on BD LSR Fortessa (BD Biosciences, USA) and analyzed with FlowJo software (version 7.6.5; Tree Star). We usually acquired 10, 000 events in FSC. CD4-FITC positive and SSC gates were used to delineate CD4+ cells, then gated with CD25-PE and CD127-PECY7 in these cells, and the acquisition gate was designed on the CD4+CD25highCD127low/− cells.
Yang M., Liu Y., Mo B., Xue Y., Ye C., Jiang Y., Bi X., Liu M., Wu Y., Wang J., Olsen N., Pan Y, & Zheng S.G. (2019). Helios but not CD226, TIGIT and Foxp3 is a Potential Marker for CD4+ Treg Cells in Patients with Rheumatoid Arthritis. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 52(5), 1178-1192.
+ Open protocol
+ Expand
7

Multicolor Flow Cytometry Analysis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The procedures used in this experiment have been published previously [25 (link),26 (link)]. Immune cells were evaluated in each experiment and treated with FcR blocking reagent (Miltenyi Biotec, Bergisch Gladbach, Germany), stained with fluorescence-labeled antibodies as indicated, and incubated for 20 min at 4 °C in phosphate buffered saline (PBS) containing 0.1% bovine serum albumin. The following mouse antibodies were purchased from BioLegend (San Diego, CA, USA): anti-CD3 (labeled with FITC, PE, and APC-Cy7), anti-CD4 (PE), anti-CD8 (FITC and APC-Cy7), anti-CD11b (APC-Cy7), anti-CD11c (BV421), anti-CD40 (APC), anti-CD44 (PE), anti-CD45 (PerCP-Cy5.5), anti-CD45.1 (Pacific Blue), anti-CD45.2 (Pacific Blue), anti-CD62L (PerCP), anti-CD69 (PE), anti-CD80 (PerCP-Cy5.5), anti-CD86 (PE), anti-CD103 (PE-Cy7), anti-CD127 (BV510), and anti-NK1.1 (PerCP-Cy5.5.). We also used 7-AAD (BioLegend) to stain dead cells for the evaluation of cell viability. The stained cells were subjected to FACS Verse for data acquisition (Becton Dickinson, San Jose, CA, USA) and analyzed using FlowJo software (Tree Star, Ashland, OR, USA).
Watanabe A., Yamashita K., Fujita M., Arimoto A., Nishi M., Takamura S., Saito M., Yamada K., Agawa K., Mukoyama T., Ando M., Kanaji S., Matsuda T., Oshikiri T, & Kakeji Y. (2021). Vaccine Based on Dendritic Cells Electroporated with an Exogenous Ovalbumin Protein and Pulsed with Invariant Natural Killer T Cell Ligands Effectively Induces Antigen-Specific Antitumor Immunity. Cancers, 14(1), 171.
+ Open protocol
+ Expand
8

Lung and Tumor Immune Cell Isolation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lymphocytes were isolated from lung and tumor tissue by digestion with collagenase A (1 mg/ml; Roche) and DNase I (0.5 µg/ml; Roche) in isolation buffer (RPMI 1640 supplemented with 5% FBS, 1% l-glutamine, 1% penicillin-streptomycin, and 10 mM Hepes) for 30 min at 37°C. Cells were filtered through 100-µm cell strainers, washed in isolation buffer, and stained in PBS supplemented with 0.25% BSA, 2 mM EDTA, and 0.1% sodium azide. Antibodies used included anti-CD45, anti-Foxp3, anti–IL-18Rα, anti-ST2, anti-CD62L, anti-CD103, anti–PD-1, anti-GITR, anti–CTLA-4, anti-KLRG1, anti-Ki67, anti-CD5, anti-NK1.1, anti-CD45R (B220), anti–IL-17, anti–IL-4, anti-IFNγ, and anti–Ly-6C (eBioscience); anti-TCRβ, anti-CD3, anti-CD4, anti-CD8, anti-CD127, anti-CD11B, anti-MHCII, and anti-Gr1 (BioLegend); anti-CD44 and anti-CD25 (Tonbo); anti–IL-5 and anti-TNFα (BD PharMingen); and anti-AREG (R&D Systems). For exclusion of dead cells, samples were first stained with Ghost Dye (Tonbo) cell viability reagent. Intracellular staining for cytokines, Foxp3, AREG, Ki-67, and CTLA-4 was performed using the Foxp3/transcription factor staining buffer set (eBioscience) as per manufacturer’s protocol. The H2-Kb OVA257–264 tetramer was obtained from the NIH Tetramer Core Facility.
Green J.A., Arpaia N., Schizas M., Dobrin A, & Rudensky A.Y. (2017). A nonimmune function of T cells in promoting lung tumor progression. The Journal of Experimental Medicine, 214(12), 3565-3575.
+ Open protocol
+ Expand
9

Comprehensive Immune Cell Phenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were pre-incubated with 1 µg/ml of FcR blocker [Miltenyi-Biotech]. For intracellular cytokine staining, cells were first stimulated with PMA [0.1 µg/ml] and ionomycin [1 µg/ml] in the presence of Brefeldin A [3 µg/ml] for 4 h at 37°C before being surface-stained, fixed, and permeabilised [eBioscience kit]. The following antibodies were used for flow cytometry staining: intracellular staining panel including anti-CD45, anti-CD3, anti-CD8, anti-IL-17A, anti-IL-22, anti-TNF-α [all from Biolegend], and anti-IFN-γ [eBioscience] antibodies; CCR panel including anti-CXCR3 and anti-CCR4 [BD Biosciences], anti-CCR6 and anti-β7 [Biolegend], anti-CCR9 [eBioscience] and anti-CCR10 [R&D Systems] antibodies; ILC panel including anti-lineage [CD34, TCRαβ, TCRγδ, BDCA-2, FCεR1a, CD123, CD1a, CD11c, CD14, CD3, CD19, CD16], anti-CD45, anti-c-Kit, anti-CRTH2, anti-CD127 [all from Biolegend], anti-CD56 and anti-CD94 [BD Pharmingen]. All antibody cocktails contained a fluorescent dye [Invitrogen/Life technologies] to discriminate dead cells during analysis. Stained cells were acquired on an LSRII flow cytometer and analysis was performed using FlowJo software [Tree Star]. For critical discrimination, cell populations were gated against corresponding isotype or fluorescence minus one [FMO] control antibody panels.
Gwela A., Siddhanathi P., Chapman R.W., Travis S., Powrie F., Arancibia-Cárcamo C.V, & Geremia A. (2017). Th1 and Innate Lymphoid Cells Accumulate in Primary Sclerosing Cholangitis-associated Inflammatory Bowel Disease. Journal of Crohn's & Colitis, 11(9), 1124-1134.
+ Open protocol
+ Expand
10

Multiparameter Flow Cytometry of Esophageal Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human fluorochrome-conjugated antibodies, anti-CD127, anti-CD4, anti-CD3, anti-CD11c, anti-Siglec-8, anti-IL17A, anti-IFNγ, anti-TNF-α, anti-IL22, anti-FOXP3, anti-IL10 all were purchased from BioLegend. Additional anti-human CD3 and anti-human CD4 were used from BD Biosciences. Anti-human IL-22BP antibody (clone 87554) and IgG2B isotype control were obtained from R&D Systems. To identify dead cells, 7-AAD staining (BioLegend) was performed. For extracellular staining, isolated hematopoietic cells from esophageal tissues were incubated for 20 min at 4 °C. Cells were acquired on a LSRII Fortessa flow cytometer (BD). Data were analyzed with FlowJo software (Treestar).
Karstens K.F., Kempski J., Giannou A.D., Pelczar P., Steglich B., Steurer S., Freiwald E., Woestemeier A., Konczalla L., Tachezy M., Reeh M., Bockhorn M., Perez D., Mann O., Lohse A.W., Roesch T., Izbicki J.R., Gagliani N, & Huber S. (2020). Anti-inflammatory microenvironment of esophageal adenocarcinomas negatively impacts survival. Cancer Immunology, Immunotherapy, 69(6), 1043-1056.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!