Balb c mice

Manufactured by BioLASCO

Sourced in Taiwan, Province of China

BALB/c mice are an inbred strain of laboratory mice commonly used in research and experimentation. They are a widely available and well-characterized mouse model known for their specific genetic and physiological characteristics.

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Lab products found in correlation

Lab diet 5001, by Land O'Lakes (1 mentions) Nod scid mice, by Jackson ImmunoResearch (1 mentions) Nod cg prkdcscidil2rgtm1wjl sz nsg, by Jackson ImmunoResearch (1 mentions) Insulin syringe, by Terumo (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Oligo d t 25 magnetic beads, by New England Biolabs (1 mentions) Rodent diet 20, by PicoLab (1 mentions) Lps from escherichia coli o55 b5, by Merck Group (1 mentions) Nanoject 2, by Drummond (1 mentions) Nod shiltj mice, by Jackson ImmunoResearch (1 mentions) Nod shiltj, by Jackson ImmunoResearch (1 mentions) Aluminium hydroxide, by Merck Group (1 mentions) Jsm 6701f, by JEOL (1 mentions) Female balb c mice, by Inotiv (1 mentions)

Spelling variants of this product

BALB/c nude mice (31 citations) Male BALB/c mice (13 citations) BALB/c-nu mice (4 citations) BALB/c (2 citations) Female BALB/c mice (2 citations) Balb/c nu/nu mice (2 citations) Female BALB/c nude mice (2 citations) Male BALB/c nude mice (2 citations)

32 protocols using balb c mice

Cigarette Smoke Extract Modulates Bone Marrow

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Eight-week-old male Balb/c mice were obtained from BioLASCO (Taipei City, Taiwan). The mice were maintained on pelleted food and water ad libitum and housed in controlled environmental conditions (22 ± 1°C and a 12 h light/dark cycle). The protocol for the animal study was approved by the Institutional Animal Care and Use Committee of Chung-Hsing University (protocol no. 108-107).
After the mice were sacrificed, femurs were dissected. Bone marrow cells were flushed with IMDM, counted, and kept in a melting ice bath until use. A total of 1 × 10⁶ bone marrow cells was resuspended in IMDM supplemented with 20% fetal calf serum, 10% conditioned medium of recombinant mouse interleukin-3 (rmIL-3), 10% citrate bovine plasma, and 1.5 mg/mL CaCl₂. The cultures were incubated with CSE (10, 100, and 500 μg/mL) for 7 days at 37°C in a humidified atmosphere of 5% CO₂ and 95% air. The formation of colonies was observed by microscopy. Colonies of at least 50 cells were scored at 40x magnification.
For cisplatin and CSE experiments, mice were intraperitoneally (i.p.) injected with three doses of cisplatin on days 1-3 and received CSE by oral gavage on days 4-10. The control group received sterile distilled water by oral gavage on days 4-10. The animals were sacrificed on day 10 to perform CFU-GM assay.

Lin S.H., Li M.H., Chuang K.A., Lin N.H., Chang C.H., Wu H.C., Chao Y.H., Lin C.C., Pan I.H., Perng M.D, & Wen S.F. (2020). Chlorella sorokiniana Extract Prevents Cisplatin-Induced Myelotoxicity In Vitro and In Vivo. Oxidative Medicine and Cellular Longevity, 2020, 7353618.

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BALB/c Mice Husbandry Protocol

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Six-week-old female BALB/c mice were obtained from BiOLASCO (Taipei, Taiwan). The mice were maintained at 22°C±2°C and 55%±10% relative humidity in a light/dark cycle (12/12 hours). The animals were housed in plastic cages and were provided the Lab Diet 5001 (PMI Nutrition International, Shoreview, MN, USA) and water ad libitum during acclimatization, preexposure, and postexposure. Approval was obtained and the animal experiments were performed in compliance with the animal and ethics review committee of the Laboratory Animal Centre at the Taipei Medical University (Taiwan).

Pan C.H., Chuang K.J., Chen J.K., Hsiao T.C., Lai C.H., Jones T.P., BéruBé K.A., Hong G.B., Ho K.F, & Chuang H.C. (2015). Characterization of pulmonary protein profiles in response to zinc oxide nanoparticles in mice: a 24-hour and 28-day follow-up study. International Journal of Nanomedicine, 10, 4705-4716.

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Immunization and Challenge of BALB/c Mice

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Five-week-old BALB/c mice were purchased from BioLASCO Taiwan Co., Ltd. After one week of a regular diet, the mice were randomly divided into four groups (n = 5 each group: (1) PBS control group; (2) CSFV E2 monovalent vaccine group; (3) PCV2 ORF2 monovalent vaccine group; and (4) CSFV E2 plus PCV2 ORF2 bivalent vaccine group). For immunization, the mice were intramuscularly injected with 0.1 mL of CSFV E2 (15 μg/mL), PCV2 ORF2 (50 μg/mL), or CSFV E2 (15 μg/mL) plus PCV2 ORF2 (50 μg/mL), whereas mice in the PBS control group was given the same volume of treatment vaccines. The dose was determined based on our previous PCV2 studies (unpublished) and publicly available information of the antigen contents of other CSFV E2 vaccines. Three weeks post-vaccination, the vaccinated mice were challenged with 10^6.3 TCID₅₀ PCV2 (Fig. S1a). At the end of experiment, the mice were sacrificed using carbon dioxide inhalation, and their blood was collected.

Chen Y.S., Lee C.Y., Wu C.C., Kao P.L., Chen T.A., Huang Y., Chung W.B., Kuo T, & Chen C. (2024). Efficacy evaluation of a bivalent subunit vaccine against classical swine fever virus and porcine circovirus type 2. Scientific Reports, 14, 2997.

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Humanized NSG Mice for Hematopoietic Studies

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NOD.Cg-Prkdc^scidIl2rg^tm1Wjl/Sz (NSG) and NOD-SCID mice were obtained from Jackson Laboratory (Bar Harbor, ME, USA) and bred in animal facility of genomic research center, Academia Sinica. Balb/c mice were purchased from BioLasco (Taiwan Co., Ltd). All mice were used 6 to 8 weeks old and housed in microisolator cages, given autoclaved food and water and maintained under specific pathogen-free mouse room at 21±2 °C with 14:10 h light: dark cycle. Mouse studies were approved by Academia Sinica Institutional animal Care and Use Committee (AS-IACUC). Male NSG mice received sublethal total body irradiation (TBI) (300 cGy), using a RS 2000 X-ray biological irradiator, between 2 to 8 hours prior to the human umbilical cord blood mononeuclear cells (CBMC) injection. Control mice were irradiated but did not received CBMC.

Hsiao Y.W., Lai T.C., Lin Y.H., Su C.Y., Lee J.J., Liao A.T., Lin Y.F., Hsieh S.C., Wu A.T, & Hsiao M. (2016). Granulysin expressed in a humanized mouse model induces apoptotic cell death and suppresses tumorigenicity. Oncotarget, 8(48), 83495-83508.

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In Vivo Biodistribution of Surface-Modified Mesoporous Silica Nanoparticles

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In vivo biodistribution images of RMSN₂₅–PEG, RMSN₂₅–PEG-TA(2:1), RMSN₂₅–PEG-TA(1:2), and RMSN₂₅-TA were obtained
from a fluorescence imaging instrument (in vivo imaging
system (IVIS), Lumina). The BALB/c mice (6-week-old) were purchased
from BioLASCO (Taiwan) and were subcutaneously implanted with 4T1
tumor cells (2 × 10⁶ cells) to establish a heterotopic
allograft model. When the diameter of the tumor reached around 5–10
mm, mice were intravenously injected with various types of MSNs at
a dose of 200 mg/kg BW. At 24 h after the injection, mice were euthanized.
Major organs (heart, liver, spleen, lungs, and kidneys), tumors, blood,
and urine, were excised for imaging, and fluorescence intensity was
recorded using an IVIS.

Chen Z.A., Wu C.H., Wu S.H., Huang C.Y., Mou C.Y., Wei K.C., Yen Y., Chien I.T., Runa S., Chen Y.P, & Chen P. (2024). Receptor Ligand-Free Mesoporous Silica Nanoparticles: A Streamlined Strategy for Targeted Drug Delivery across the Blood–Brain Barrier. ACS Nano, 18(20), 12716-12736.

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Evaluating Ketamine's Effects on Cachexia

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The pre-clinical animal study (Protocol No. 107017) was approved by the Animal Care and Use Committee of the National Chiayi University, Chiayi, Taiwan. A total of 12 healthy 8-week old male Balb/c mice were acquired from BioLASCO Co., Ltd., Taiwan, and were raised in an environmentally controlled (12:12 dark–light cycle with a constant room temperature of 25 ± 1 °C) specific pathogen free facility. Mice were initially weighed and the 4 animals with lowest body weight were purposely assigned into the normal control group, while the remaining heavier animals were randomly assigned into 4 groups: 1 group for the chemotherapy agent 5-fluorouracil (5-Fu), and 3 for the ketamine-treated groups (mock group (saline, 0 mg/kg BW), low-dose (15 mg/kg BW), and high-dose (30 mg/kg BW)). Each group had 4 animals in one standard plastic cage fed ad libitum. For animal welfare considerations of cachexia condition, each cage had a water bottle with a stainless cap and extended tip (closer to floor). In addition, standard rodent chow LabDiet™ 5001 was smashed into loose particles and provided in a shallow glass dish on the floor close to the tip of the water bottle. Individual body weight, group feed, and water consumption were measured every morning.

Wang J., Lin Z.C, & Weng B.B. (2022). A Pre-Clinical Study of Sub-Anesthetic Ketamine as Remedy in 5-Fluorouracil-Induced Cachexia Model. Life, 13(1), 8.

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Murine Allergic Sensitization Protocol

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Balb/c mice were purchased from BioLASCO (BioLASCO Taiwan Co., Ltd., Taiwan). All mice were bred and maintained on a 12-12 light–dark cycle in the animal center of Chung Shan Medical University, and the temperature was maintained at 22–24 °C and the humidity was 55–60%.
The mice sensitization and analysis were performed with reference to previous studies [38 (link),39 (link)]; briefly, female Balb/c (6–8 weeks old) were intraperitoneally injected with different respective allergens (50 μg) in the first 3 days, and intra-nasal allergens were administered (50 μg) on days 14, 17, 21, 24, and 27 (Figure 4A).

Hu R.H., Cheng C.W., Wu C.T., Ko J.L., Lue K.H, & Liu Y.F. (2022). Integrated OMICs Approach for the Group 1 Protease Mite-Allergen of House Dust Mite Dermatophagoides microceras. International Journal of Molecular Sciences, 23(7), 3810.

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Balb/c Mice Experimental Protocol

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Eight-week-old female Balb/c mice (BioLASCO Taiwan Co., Ltd.) were used in this experiment. Animal study protocols for the in vivo experiments herein were reviewed and approved by the Institutional Animal Care and Use Committee (IACUC) of National Health Research Institutes, Taiwan.

Yang C.W., Hsu H.Y., Chang H.Y., Lee Y.Z, & Lee S.J. (2020). Natural cardenolides suppress coronaviral replication by downregulating JAK1 via a Na+/K+-ATPase independent proteolysis. Biochemical Pharmacology, 180, 114122.

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Hepatocellular Carcinoma Mouse Model with Combination Therapy

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Five-week-old male severe-combined immune deficient (SCID) or BALB/c mice were purchased from BioLASCO Company (Yi Lan, Taiwan, ROC) and were housed in a specific pathogen free condition at China Medical University Laboratory Animal Center. Food and water were available ad libitum. All animal experiments were approved by the Laboratory Animal Care and Use Committee of the China Medical University. After anesthesia, mice underwent median laparotomy. Huh7 (5 × 10⁵) or ML-1 cell (10⁵) were inoculated into the subcapsular parenchyma of the left liver lobe by an insulin syringe (Terumo, Elkton, MD). One week after inoculation, mice were randomly assigned to 4 groups. To evaluate the combination therapy, mice were administered DNA/liposome complex (20 μg) twice per week and then Dox (0.5 mg/kg) once per week by tail vein injection for a total of 4 weeks. Tumor growth was monitored weekly by in vivo Imaging System (Xenogen). Thirty-seven days after the initial treatment, all mice were sacrificed and liver tumors dissected and weighed.

Dai H.Y., Chen H.Y., Lai W.C., Hung M.C, & Li L.Y. (2015). Targeted expression of BikDD combined with metronomic doxorubicin induces synergistic antitumor effect through Bax activation in hepatocellular carcinoma. Oncotarget, 6(27), 23807-23819.

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Nanopore Direct RNA Sequencing of Coronavirus Infection

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For nanopore direct RNA sequencing in vitro, HRT-18 and ML cells were infected with BCoV and MHV-A59 at a multiplicity of infection (MOI) of 0.1, and total cellular RNA was collected at 24 and 20 h postinfection, respectively. For nanopore direct RNA sequencing in vivo, 3-week-old male and specific pathogen-free BALB/c mice (BioLASCO Taiwan Co., Ltd.) were infected by intraperitoneal inoculation of 10⁶ PFU of MHV-A59 in 500 µl of DMEM. The livers of MHV-A59-infected mice were collected at 3 days postinfection. TRIzol (Thermo Fisher Scientific, Waltham, USA) was used to collect total cellular RNA from MHV-A59-infected cells and MHV-A59-infected livers according to the manufacturer’s instructions. Poly(A)-tailed RNA used for nanopore direct RNA sequencing was obtained using oligo d(T)25 magnetic beads (New England Biolabs, USA) according to the manufacturer’s instructions.

Lin C.H., Chen B., Chao D.Y., Hsieh F.C., Lai C.C., Wang W.C., Kuo C.Y., Yang C.C., Hsu H.W., Tam H.M, & Wu H.Y. (2023). Biological characterization of coronavirus noncanonical transcripts in vitro and in vivo. Virology Journal, 20, 232.

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