Myc sirna

HeLa cells and MDA-MB-231 cells (whether unmodified or CRISPR-modified cell lines), grown to around 30% or 50% confluency in 12-well plates, respectively, were treated with vehicle (DMSO; 0.1% (v/v), the final concentration in all compound-treated samples) or compound of interest in growth medium for 24 h. After 24 h, the medium was removed, and the cells were treated with fresh medium containing compound for additional 24 h. For MYC siRNA studies, cells were seeded in 12-well plates and 1 nM of MYC siRNA (SantaCruz, sc-29226) or scrambled control (SantaCruz, sc-37007) was transfected with Lipofectamine 3000 according to the manufacturer’s recommended protocol. Namalwa (0.4 × 10⁶ cells per ml, 2 ml), HL-60 (0.5 × 10⁶ cells per ml, 2 ml) and Raji (0.7 × 10⁶ cells per ml, 2 ml) cells were treated with vehicle, MYC-RIBOTAC or MYC-Ctr for 48 h. For Namalwa, HL-60 and Raji cells, RNase L mRNA levels were also measured using RT–qPCR. For studies performed in MCF-10a and MDA-MB-231 cells, TaqMan assays were also performed using the TaqMan gene expression assay (GAPDH: Hs03929097_g1, 4331182; MYC: Hs00153408_m1, 4331182) and the TaqMan Fast Advanced MasterMix.