Fluorescein acrylate

Manufactured by Merck Group

Fluorescein acrylate is a fluorescent dye molecule that can be used as a labeling reagent in various analytical and research applications. It has the chemical formula C₂₀H₁₂O₅ and is a derivative of the fluorescein compound. The primary function of fluorescein acrylate is to provide a fluorescent signal that can be detected and measured, enabling the tracking or visualization of labeled molecules or structures.

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2 protocols using fluorescein acrylate

Synthesis of Fluorescent Hydrogel Scaffolds

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Poly(ethylene glycol) methacrylate (EHEMA5, Mn=360), tetraethylene glycol dimethacrylate (TTEGDMA), azobisisobutryonitrile (AIBN), mercapto ethanol, dibutyltin dilaurate, hexamethylene diisocyanate (HMDI), triphenylphosphine (TPP), dimethoxyphenylacetophenone (DMPA) and fluorescein acrylate were received from Sigma-Aldrich. Methacryloxyethyl thiocarbamoyl rhodamine B (PolyFluor 570) was received from Polysciences. Allyl isocyanate (AI) was received from TCI America. Methyl ethyl ketone (MEK), methanol, ethanol and methanesulfonic acid (MsOH) were received from Fisher. Dexamethasone and divinyl sulfone (DVS) were received from VWR. Pentaerythritol tetrakis(3-mercaptopropionate) (PETMP) and ethoxylated trimethylolpropane tri(3-mercaptopropionate) (ETTMP, Mn=1300) were donated by Evans Chemetics. AIBN was recrystallized from methanol. All other materials were used as received.

Dailing E.A., Nair D.P., Setterberg W.K., Kyburz K.A., Yang C., D’Ovidio T., Anseth K.S, & Stansbury J.W. (2015). Combined, Independent Small Molecule Release and Shape Memory via Nanogel-Coated Thiourethane Polymer Networks. Polymer chemistry, 7(4), 816-825.

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Synthesis of Fluorescent PEG Hydrogels

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Pyridine and other organic solvents were purified as reported by Riddick et al. [46 ]. Poly(ethylene glycol) (PEG-9) was supplied by Merck Chemical Co.; acrylic acid and fluorescein acrylate were supplied by Sigma-Aldrich. Colemanite (Ca₂B₆O₁₁·5H₂O) was obtained from ETI Mine Works General Management (Turkey). Iron(III) oxide, hydrochloric acid, and nitric acid were purchased from Sigma-Aldrich. Highly pure NH₃ gas (99.98%) was provided by Schick GmbH & Co. KG. All solutions were prepared with distilled water.

Kalay S., Stetsyshyn Y., Donchak V., Harhay K., Lishchynskyi O., Ohar H., Panchenko Y., Voronov S, & Çulha M. (2019). pH-Controlled fluorescence switching in water-dispersed polymer brushes grafted to modified boron nitride nanotubes for cellular imaging. Beilstein Journal of Nanotechnology, 10, 2428-2439.

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