Sodium new houttuyfonate (SNH) was purchased from Xi'an Kailai Biological Engineering Co., Ltd. (Xi'an, China). We used high performance liquid chromatography to quantitatively analyze SNH with a purity of 98% (Fig. S1† ). HepG2 cells were obtained from ATCC. Dulbecco's Modified Eagle Medium (DMEM) was purchased from Gibco (USA). Fetal bovine serum (FBS), MTT, penicillin and streptomycin all were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Annexin V-FITC/PI apoptosis detection kit, Apoptosis-Hoechst staining kit and DNA ladder extraction kit were from Shanghai Beyotime Biological Technology Co. Ltd. (Shanghai China). The primer of GADPH, MMP9, VEGF and Bcl-2 were synthesized by Shanghai Sangon Biological Engineering Technology and Service Company. One step RT-PCR kit was bought from Qiagen (Germany). All other reagents were of the highest grade commercially available.
Mmp 9
Manufactured by Sangon
Sourced in China
MMP-9 is a lab equipment product used to measure the level of matrix metalloproteinase-9 (MMP-9) in biological samples. MMP-9 is an enzyme involved in the breakdown of extracellular matrix and plays a role in various physiological and pathological processes. The MMP-9 product provides a tool for researchers to quantify and analyze MMP-9 levels in their studies.
Automatically generated - may contain errors
Lab products found in correlation
Il 1β, by Sangon (4 mentions)
β actin, by Sangon (3 mentions)
Gapdh, by Sangon (3 mentions)
Interleukin 6 (il 6), by Sangon (3 mentions)
Mmp 3, by Sangon (3 mentions)
Tnf α, by Sangon (3 mentions)
Mmp 1, by Sangon (2 mentions)
Anti timp metallopeptidase inhibitor 1 timp 1, by Abcam (2 mentions)
Ccl 2, by Sangon (2 mentions)
Timp 1, by Sangon (2 mentions)
Il 17a, by Sangon (2 mentions)
Ccl 3, by Sangon (2 mentions)
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)
Hepg2 cell, by American Type Culture Collection (1 mentions)
Penicillin, by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Onestep rt pcr kit, by Qiagen (1 mentions)
Gadph, by Sangon (1 mentions)
Vascular endothelial growth factor (vegf), by Sangon (1 mentions)
Bcl 2, by Sangon (1 mentions)
9 protocols using mmp 9
1
Quantitative Analysis of Sodium New Houttuyfonate in HepG2 Cells
2
Antarctic Diatom Compound Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Antarctic diatom (Phaeodactylum tricornutum ICE-H) used in this experiment was isolated and purified from seawater and sea ice near the Zhongshan Research Station of Antarctica (S 69°48′, E 77°48′) during China’s 18th Antarctic Scientific Expedition [54 (link)]. Phaeodactylum tricornutum ICE-H was cultivated in a low-temperature light incubator after being infected in a triangle flask with f/2 media [55 (link)] at a 30% inoculation level. The light cycle was 12 L:12 D, the light intensity was 40 μmol m−2 s−1, and the culture temperature was 4 ± 1 °C. The reagent kits for the measurement of ROS, MDA, Hyp, ABTS, and DPPH were purchased from Servicebio Technology Co., Ltd. (Wuhan, China). SPF male Kunming (KM) mice were purchased from Pengyue Experimental Animal Breeding Co., Ltd. (Jinan, China). The primers targeting mRNAs, including IL-1β, TNF-α, NF-κB, COX-2, MMP-1, MMP-9, and β-actin, were synthesized by Sangon Biotech Co., Ltd. (Shanghai, China).
3
Circular RNA circ_0084043 Regulates Cell Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and phosphate buffered saline (PBS) (pH = 7.2) were purchased from Gibco (Shanghai, China); ethanol (70%), isopropyl alcohol, and Triton X-100 were bought from Sigma-Aldrich (Shanghai, China); Cell Counting Kit-8 (CCK-8) were bought from Dongren Chemical Technology (Shanghai, China); bicinchonininc acid (BCA) protein assay kit was purchased from Thermo Fisher Scientific (Shanghai, China); antibodies were purchased from Cell Signaling Technology (Shanghai, China) and Abcam (Shanghai, China); the primers [circ_0084043, PCDH9, RAC1, Cyclin D1, Pyk2, FAK, MMP2, MMP9, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH)] were designed and synthesized by Sangon Biotech (Shanghai, China); the small interfering ribonucleic acid (siRNA) for circ_0084043 (si-circ_0084043) was designed and synthesized by Ribo Biotech (Guangzhou, China); the miR-134-5p mimics, miR-134-5p inhibitor, and miRNA negative control (miR-NC) were also purchased from Ribo Biotech; GV358-PCDH9 lentivirus and GV358-siRNA lentivirus were designed by Genechem (Shanghai, China); SYBR® Premix Ex Taq™ II and PrimeScript™ RT reagent Kit with genomic DNA (gDNA) Eraser were bought from Takara Bio Inc. (Beijing, China); ultrapure water was obtained from EPED-20TF (Nanjing, China).
4
Evaluating Inflammatory Markers in Bone Regeneration
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Complete Freund’s adjuvant (CFA) was obtained from Chondrex, Inc. (USA). D-luciferin was purchased from Abcam (UK). Rabbit anti-BMP3, anti-TNF-a, and anti-TIMP metallopeptidase inhibitor 1 (TIMP-1) antibodies were obtained from Abcam (UK). Rabbit anti-MMP-3 and anti-MMP-9 antibodies were acquired from Merck Millipore (USA). Rabbit anti-IL-6, anti-IL-1β, and anti-IL-17A were purchased from Bioworld (USA). The rat anti-β-actin antibody was purchased from CST (USA). A horseradish peroxidase (HRP)-labeled goat anti-rabbit immunoglobulin (IgG) was procured from Zhongshan Biotechnology Corporation (China). BMP3, MMP-3, MMP-9, TIMP-1, TNF-a, IL-6, IL-1β, IL-17A, CCL-2, CCL-3, VCAM-1, and β-actin primers were synthesized by the Shanghai Sangon Biological and Technological Company (China).
5
Quantitative RT-PCR Analysis of OSCC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The TRIzol reagent (ComWin Biotech Co., Ltd., Beijing, China) was used to extract the total RNA from the transfected OSCC cells, and the Super RT cDNA Synthesis Kit (ComWin) was used to perform the reverse transcription reaction. PCR was performed using the ULtraSYBR Mixture (Low ROX) (ComWin). The primer sets for human RhoC (catalog number: QRP20382) was provided by GeneCopoeia (Rockville, MD, USA). The primer sets of GAPDH (forward, 5′CATGGGTGTGAACCATGAGAAGTAT-3′; reverse, 5′GACTGTGGTCATGAGTCCTTCCA-3′), MMP-9 (forward, 5′TGTACCGCTATGGTTACACTCG-3′; reverse, 5′GGCAGGGACAGTTGCTTCT-3′), and Src (forward, 5′ATCACCGCAAGAGCTACCAT-3′; reverse, 5′TGACGGTGTCCGAGGAGTTG-3′) were provided by Sangon Biotech Co., Ltd. (Shanghai, China). The PCR results were analyzed using the 2−ΔΔCt method.
6
Quantitative Real-Time PCR Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA was extracted from cells using an Omega R6934‐01 Total RNA Kit (Omega Bio‐tek, Norcross, GA, USA) according to the manufacturer's protocol. cDNA synthesis was performed using PrimeScript RT Reagent (DRR037A; Takara Bio, Shiga, Japan) following the manufacturer's instructions. qPCR was performed on a Q3 LightCycler 2.0 instrument using SYBR Premix Ex Taq (DRR081A; Takara Bio), and results were calculated using the 2−ΔΔCt method 27 . The MMP‐1, MMP‐2, MMP‐9, and GAPDH primers were synthesized by Shanghai Sangon Co (Shanghai, China) as described previously 28 , and the LGALS3 and MGAT5 primers were synthesized by Beijing Genomics Institute (BGI, Shenzhen, China).
7
Investigating MMP and METTL3 Regulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Rabbit anti-MMP3, anti-MMP9, and anti-METTL3 antibodies were obtained from Abcam (Cambridge, UK). We obtained the rabbit anti-phospho-(p)-p65 antibody, anti-β-actin antibody, anti-vimentin antibody, and anti-p65 antibody from Cell Signaling Technology, Inc. (Danvers, MA, USA). Rabbit anti-proliferating cell nuclear antigen (PCNA) antibody was obtained from Bioss (Beijing, China). The secondary antibody we used was from Zhongshan Biotechnology Corporation (Beijing, China). The cell cycle kits we used were from BestBio (Shanghai, China). The enzyme-linked immunosorbent assay (ELISA) kits for IL-6 were purchased from Elabscience Biotechnology Co., Ltd. METTL3, MMP3, MMP9, PCNA, IL-6, IL-1β, and TNF-α primers were synthesized by Shanghai Sangon Biological and Technological Company (Shanghai, China).
8
Cytokine Profiling of C6 Glioma Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We measured tumor-derived secreted cytokines according to the manufacturer's instructions. VEGF (ExCell Biology, Inc., Shanghai, China), MMP-2 and MMP-9 (Sangon Biotech, Shanghai, China) in the TCM or ICM of C6 glioma cells were detected by enzyme-linked immunosorbent assay (ELISA).
9
PTEN and 5-Aza Modulate Inflammatory Response
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Bpv (PTEN) and 5-Aza-2′-deoxycytidine (5-Aza) were purchased from Sigma-Aldrich, Inc. (St. Louis, MO). Complete Freund's adjuvant was obtained from Chondrex, Inc. (Redmond, WA). Rabbit anti-PTEN, anti-TIMP metallopeptidase inhibitor 1 (TIMP-1), anti-TNF-α antibody and mouse anti-DNA methyltransferase 1(DNMT1) monoclonal antibody were purchased from Abcam (Cambridge, UK). Rabbit anti-IL-1β, anti-IL-6 and anti-IL-17A, and mouse anti-β-actin monoclonal antibody were obtained from Bioworld (Shanghai, China). Rabbit anti-MMP-3, anti-MMP-9, anti-AKT and anti-p-AKT antibody, and Simple ChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) were purchased from Cell Signaling Technology (Danvers, MA). Peroxidase-Conjugated Goat anti-Rabbit IgG (H+L) were purchased from ZSGB-BIO (Beijing, China). The primers of PTEN, IL-1β, IL-6, IL-17A, IL-8, IL-10, MMP-3, MMP-9, TIMP-1, CCL-2, CCL-3, CCL-8 and β-actin were synthesized by Sangon Biotech Co., Ltd (Shanghai, China).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!