TCMK-1, HEK-293T, and RAW264.7 macrophage were acquired from the American Type Culture Collection (Manassas, VA). TCMK-1, HEK-293T and RAW264.7 cells were cultured in DMEM (Sigma-Aldrich) containing 10% fetal bovine serum (FBS) and incubated at 37 °C in a humidified atmosphere of air/CO2 (95:5). For IL-1β-induced aging research, TCMK-1 and HEK-293T were incubated in MEM medium supplemented with different doses of IL-1β for 24 h. For assessing the treatment effect of IL-1β antibody, cells were treated with IL-1β (5 ng/ml) or cell culture supernatant with IL-1β antibody (5 µg/ml) at the same time for 24 h. For TGFβ-induced fibrosis research, HEK-293T and primary tubular epithelia cells were exposed to TGFβ (20 ng/ml) for 48 h. For plasmid transfection research, gene-expressing plasmid and blank plasmid (pVector) were obtained from MiaoLing Plasmid (Wuhan, China). TCMK-1 and HEK-293T cells transfection with plasmids was conducted using Lipofectamine 2000 (12566014, Invitrogen, CA, USA) for 24 h, according to the manufacturer’s instructions.
Tcmk 1
Manufactured by Merck Group
The TCMK-1 is a laboratory equipment designed for cell and tissue culture applications. It provides a controlled environment for the maintenance and growth of cell cultures. The core function of the TCMK-1 is to regulate temperature, humidity, and atmospheric conditions to support the optimal growth and health of cultured cells.
Automatically generated - may contain errors
Lab products found in correlation
Tcmk 1, by American Type Culture Collection (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Hek293t, by Merck Group (1 mentions)
Tcmk 1, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Hek293t, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions)
Hek293t, by American Type Culture Collection (1 mentions)
Raw264.7 macrophage, by American Type Culture Collection (1 mentions)
Controlled hypoxic chamber, by Thermo Fisher Scientific (1 mentions)
N acetylcysteine nac, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium f12, by Thermo Fisher Scientific (1 mentions)
2 protocols using tcmk 1
1
Cell Culture Techniques for Aging and Fibrosis
2
Hypoxia-Reoxygenation and Oxidative Stress in Kidney Cells
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The TCMK-1 (murine tubular epithelial cells) and human kidney 2 (HK2) (proximal tubular cells) cell lines were obtained from the American Type Culture Collection (ATCC; Manassas, VA). Cells were cultured in Dulbecco's modified Eagle's medium/F12 (Thermo Scientific, Rockford, IL) supplemented with 10% fetal bovine serum (Thermo Scientific) at 37 °C in 5% CO 2 . For H/R research, confluent TCMK-1 or HK2 cells were incubated under hypoxic conditions (1% O 2 , 94% N 2 , and 5% CO 2 ) in a controlled hypoxic chamber (Thermo Scientific) for 24 h, and then removed or not to a normoxic incubator for reoxygenation. Normoxic controls were cultured under normoxic conditions (21% O 2 , 5% CO 2 , 74% N 2 ) for identical times. For the H 2 O 2 study, confluent TCMK-1 cells were exposed or not to H 2 O 2 at different doses for 24 h. In certain experiments, cells were pre-administered different doses of recombinant Klotho protein or 30 µM N-acetylcysteine (NAC; Sigma-Aldrich) for 1 h.
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