Alexa fluor 488 conjugated goat anti chicken igg

Manufactured by Thermo Fisher Scientific

Sourced in United States

Alexa Fluor 488 conjugated goat anti-chicken IgG is a secondary antibody that binds to chicken immunoglobulin G (IgG). It is conjugated to the Alexa Fluor 488 fluorescent dye, which emits green fluorescence when excited by a suitable light source. This product can be used for immunofluorescence applications to detect and visualize chicken IgG in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

8 protocols using alexa fluor 488 conjugated goat anti chicken igg

3D Culture and ROS Imaging of MCF-10A Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

3D culture of MCF-10A cells was performed as described (Debnath et al., 2003 (link)). Briefly, 10⁴ cells were seeded onto one chamber of a 4-well chamber slide pre-coated with Matrigel (BD Biosciences). After 31 days, cells were fixed using 4% paraformaldehyde (Fisher) and permeabilized using 0.5% Triton X-100, and sequentially incubated with anti-Laminin-5 (D4B5, EMD Millipore), Alexa Fluor 568 conjugated goat anti-mouse IgG (Invitrogen), anti-GFP (ab13970, Abcam), Alexa Fluor 488 conjugated goat anti-chicken IgG (Invitrogen), and nuclear stain 4,6-diamidino-2-phenylindole (DAPI, Sigma). Images were acquired using the Leica TCS SP5-II Confocal Upright and the PerkinElmer UltraVIEW ERS confocal microscopes at the Molecular Cytology Core Facility at MSKCC and analyzed by MetaMorph software (Molecular Devices). To measure ROS levels, 3D culture of MCF-10A cells after 6-8 days were incubated with Hank's Balanced Salt Solution (HBSS, Invitrogen) containing 5 μM dihydroethidium (DHE, Invitrogen) and 3 μg/ml Hoechst 33342 (Invitrogen) at 37°C for 30 minutes in a humidified 5% CO₂ incubator, washed once with HBSS, then immediately imaged.

Wang G.X., Tu H.C., Dong Y., Skanderup A.J., Wang Y., Takeda S., Ganesan Y.T., Han S., Liu H., Hsieh J.J, & Cheng E.H. (2017). ΔNp63 Inhibits Oxidative Stress-Induced Cell Death Including Ferroptosis and Cooperates with the BCL-2 Family to Promote Clonogenic Survival. Cell reports, 21(10), 2926-2939.

+ Open protocol

+ Expand

Immunofluorescence Staining of Neural Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fixed coronal brain sections, neurospheres, or single NSC were permeabilized with 0.1% Triton X-100 for 10 min, blocked with 5% fetal bovine serum in 0.1% Triton X-100 for 1 h at room temperature, and then incubated with primary antibodies at 4 °C overnight. Incubation with the appropriate secondary antibodies or FITC-Phalloidin (1:200, Sigma) and DAPI (1:10,000, Molecular Probes, USA) was performed at room temperature for 1 h. Glass slides were mounted using Fluoromount G mounting medium (Electron Microscopy Sciences, USA). Brain images were captured on a Leica TCS SP8 confocal microscopy using LASAF software (Leica, Germany), and NSC images were captured on an Olympus FluoView 300 confocal system using the FluoView software (Olympus, Japan).
Primary antibodies: mouse anti-CSPG (1:250, Abcam, USA); guinea pig anti-DCX (1:1000, Millipore, USA); chicken anti-GFAP (1:500, Abcam). Secondary Antibodies (Invitrogen, USA): Alexa Fluor 594-conjugated goat anti-mouse IgG (1:300); Alexa Fluor 488-conjugated goat anti-guinea pig IgG (1:1000); Alexa Fluor 488-conjugated goat anti-chicken IgG (1:500).

Galindo L.T., Mundim M.T., Pinto A.S., Chiarantin G.M., Almeida M.E., Lamers M.L., Horwitz A.R., Santos M.F, & Porcionatto M. (2017). Chondroitin Sulfate Impairs Neural Stem Cell Migration Through ROCK Activation. Molecular Neurobiology, 55(4), 3185-3195.

+ Open protocol

+ Expand

Immunohistochemical Analysis of Dystrophin, Laminin, and GFP

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tissue sections were re-warmed for 30 min at RT and blocked with 5% BSA for 1 h at RT, followed by incubation overnight at 4 °C with one or two of the following primary antibodies: rabbit anti-dystrophin (1:200; Abcam), rabbit anti-laminin (1:200; Abcam), and chicken anti-GFP (1:1000; Abcam). After washing, the tissue sections were incubated for 1 h at RT with one or two of secondary antibodies: Alexa Fluor 350-conjugated goat anti-rabbit IgG (1:1000; GeneCopoeia, Rockville, MD, USA) and Alexa Fluor 488-conjugated goat anti-chicken IgG (1:1000; Invitrogen). Immunoreactivity was visualised using a fluorescence microscope (ECLIPSE Ni-U; Nikon, Tokyo, Japan).

Wang L., Li H., Lin J., He R., Chen M., Zhang Y., Liao Z, & Zhang C. (2021). CCR2 improves homing and engraftment of adipose-derived stem cells in dystrophic mice. Stem Cell Research & Therapy, 12, 12.

+ Open protocol

+ Expand

Immunofluorescence Labeling for Cryosectioned Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols

Aforementioned cryosections (4 μm) were placed on slide glasses (refer to the text of H&E staining). The sections were incubated with either 3% normal goat serum or 3% normal donkey serum and 0.5% Triton X-100 in PBS for 30 min to block non-specific binding, and then incubated with primary antibodies diluted in blocking solution overnight at 4 °C (see Supplementary Table S1 online). The sections were then incubated with secondary antibodies for 1 h (1:1000 dilution) at room temperature (22–25 °C). Alexa Fluor-488-conjugated donkey anti-goat IgG, Alexa Fluor-488-conjugated goat anti-rat IgG, Alexa Fluor-488-conjugated goat anti-chicken IgG, Alexa Fluor-568-conjugated donkey anti-rat IgG, Alexa Fluor-647-conjugated donkey anti-chicken IgG, Alexa Fluor-568-conjugated donkey anti-rabbit IgG (all 1:500 dilution; Invitrogen, USA) were used as secondary antibodies. After washes with PBS, the immunolabeled sections were mounted using PermaFluor aqueous mounting medium (PermaFluor, Thermo Scientific, USA) and were observed under a CLSM (FV1000, Olympus, Japan) with following acquisition parameters: excitation at 473 and 559 nm, × 60 water immersion lens (NA = 1.2), image size = 105 × 105 μm.

Hiroshige T., Uemura K.I., Hirashima S., Hino K., Togo A., Ohta K., Igawa T, & Nakamura K.I. (2021). Identification of PDGFRα-positive interstitial cells in the distal segment of the murine vas deferens. Scientific Reports, 11, 7553.

+ Open protocol

+ Expand

Multicolor Immunofluorescence in Barrel Cortex

Check if the same lab product or an alternative is used in the 5 most similar protocols

Barrel cortex sections were washed in TRIS buffer (TB) 1 × 15 min, TRIS-buffered saline (TBS) 1 × 15 min, and TBS + 0.5% Triton X-100 (TBST) 2 × 15 min, all at pH 7.6. For blocking, sections were incubated 90 min at room temperature in 0.25% bovine serum albumin/10% goat serum/TBST (Jackson ImmunoResearch). For primary antibody labeling, sections were incubated 48–72 h at 4 °C with (1) chicken anti-GFP (Aves; Cat#GFP-1020, RRID:AB_10000240) diluted 1:500 and (2) mouse anti-RFP (Rockland; Cat#200–301-379S, RRID:AB_2611064) diluted 1:2000. Sections were washed 4 × 15 min with TBST. For secondary antibody labeling, sections were incubated 4 h at room temperature with (1) Alexa Fluor 488-conjugated goat anti-chicken IgG (Molecular Probes; Cat#A11039) and (2) Alexa Fluor 568-conjugated goat anti-mouse IgG2a (Molecular Probes; Cat#A21134) diluted 1:500 in TBST. In some cases, sections were stained in addition with the primary antibody guinea pig anti-vGluT2 (Millipore; Cat#AB2251, RRID: AB_1587626) diluted 1:2000 and the secondary antibody Alexa Fluor 633-conjugated goat anti-guinea pig (Molecular probes; Cat#A21105) diluted 1:500. After washing 2 × 15 min with TBST and 1 × 15 min with TBS, sections were stained with DAPI, diluted 1:1000 in TBS. After several washes in TB, sections were mounted in Aqua-Poly-Mount (Polysciences).

Hafner G., Guy J., Witte M., Truschow P., Rüppel A., Sirmpilatze N., Dadarwal R., Boretius S, & Staiger J.F. (2020). Increased Callosal Connectivity in Reeler Mice Revealed by Brain-Wide Input Mapping of VIP Neurons in Barrel Cortex. Cerebral Cortex (New York, NY), 31(3), 1427-1443.

+ Open protocol

+ Expand

Immunohistochemical Labeling of Barrel Cortex

Check if the same lab product or an alternative is used in the 5 most similar protocols

Barrel cortex sections were washed in TRIS buffer (TB) for 15 min, TRIS-buffered saline (TBS) for 15 min and TBS + 0.5% Triton X-100 (TBST) for 2×15 min, all at pH 7.6. Blocking was done for 90 min at room temperature in 0.25% bovine serum albumin/10% goat serum/TBST (Jackson Immuno Research). Sections were incubated for 48–72 h at 4°C with primary antibodies (i) chicken anti-GFP (Aves) diluted 1:1000, (ii) mouse anti-RFP (Rockland) diluted 1:1000, and (iii) rabbit anti-PV (Swant) diluted 1:5000 in blocking solution. After washing 4×15 min with TBST, secondary antibodies (i) Alexa Fluor 488-conjugated goat anti-chicken IgG, (ii) Alexa Fluor 568-conjugated goat anti-mouse IgG2a, and (iii) Alexa Fluor 633-conjugated goat anti-rabbit (Molecular Probes) were diluted 1:500 in TBST and sections were incubated for 4h at room temperature. After washing 2×15 min with TBST and 1×15 min with TBS, sections were stained with DAPI, diluted 1:1000 in TBS. After several washes in TB, sections were mounted in Aqua-Poly-Mount (Polysciences).
For staining against oG, blocking solution was prepared with 3% bovine serum albumin/10% goat serum/TBST. Guinea pig anti-rabies glycoprotein antibody (kindly donated by A. Lüthi) was diluted 1:500 in blocking solution and combined with Alexa Fluor 633-conjugated goat anti-guinea pig IgG (Molecular Probes).

Hafner G., Witte M., Guy J., Subhashini N., Fenno L.E., Ramakrishnan C., Kim Y.S., Deisseroth K., Callaway E.M., Oberhuber M., Conzelmann K.K, & Staiger J.F. (2019). Mapping Brain-Wide Afferent Inputs of Parvalbumin-Expressing GABAergic Neurons in Barrel Cortex Reveals Local and Long-Range Circuit Motifs. Cell reports, 28(13), 3450-3461.e8.

+ Open protocol

+ Expand

Immunohistochemistry of GFP in Mouse Brain

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice were killed 2, 4 or 8 weeks after the injection. Anesthetized mice were transcardially perfused with PBS followed by 4% PFA. Brains were dissected and post-fixed in 4% PFA overnight at 4 °C. 50-μm coronal sections were obtained with a vibratome (Leica Microsystems). Antigen retrieval was performed as follows: sections were incubated for 30 min at 80 °C in 50 mM sodium acetate solution. Then the slices were washed three times in a PBTriton 0.1% solution. Sections were incubated with chicken anti-GFP (Abcam; 1:500) primary antibody overnight at 4 °C and then rinsed in PBTriton 0.1%. Alexa Fluor 488–conjugated goat anti-chicken IgG (1:500; Life Technologies) was used overnight at 4 °C as the secondary antibody. The next day, sections were washed three times with PBTriton 0.1% solution and counterstained with DAPI. High-power confocal images in the injection site of the DRN region were obtained on a Nikon A1 confocal microscope with a 10× or 40× plan-apochromat.

Alberio L., Locarno A., Saponaro A., Romano E., Bercier V., Albadri S., Simeoni F., Moleri S., Pelucchi S., Porro A., Marcello E., Barsotti N., Kukovetz K., Boender A.J., Contestabile A., Luo S., Moutal A., Ji Y., Romani G., Beltrame M., Del Bene F., Di Luca M., Khanna R., Colecraft H.M., Pasqualetti M., Thiel G., Tonini R, & Moroni A. (2018). A light-gated potassium channel for sustained neuronal inhibition. Nature methods, 15(11), 969-976.

+ Open protocol

+ Expand

Cardiac Protein Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary antibodies used were: SLN for Western blot (Merck Millipore, #ABT13), SERCA2a (Abcam, #ab2861), PLB (Cell Signaling, #14562), phospho-PLB(Ser16) (Santa Cruz, SC-12963-R), phospho-PLB(Thr17) (Santa Cruz, SC-17024-R), FLAG (Sigma, F3165), GAPDH (Santa Cruz, sc-47724). Secondary antibodies for immunoblotting were HRP-conjugated donkey anti-chicken (Invitrogen), rabbit anti-mouse or goat anti-rabbit IgG (Jackson ImmunoResearch). Secondary antibodies for immunofluorescence were Alexa Fluor 488 conjugated goat anti-rabbit IgG, Alexa Fluor 568-conjugated goat anti-mouse IgG, Alexa Fluor 488-conjugated goat anti-chicken IgG (Life Technologies).

Morales Rodriguez B., Domínguez-Rodríguez A., Benitah J.P., Lefebvre F., Marais T., Mougenot N., Beauverger P., Bonne G., Briand V., Gómez A.M, & Muchir A. (2020). Activation of sarcolipin expression and altered calcium cycling in LMNA cardiomyopathy. Biochemistry and Biophysics Reports, 22, 100767.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!