The invasion assay was performed using a 24-well plate transwell chamber containing 8-μm wells (Corning, USA). The top chamber contained Matrigel (BD Biosciences). Cells (1 × 105/well) were inoculated in a serum-free medium in the top chamber, while the bottom chamber contained the medium, along with 10% FBS. The invasion of cells was allowed to occur for 48 h at 37°C, after which they were fixed with 4% paraformaldehyde and stained with 0.1% crystal violet solution. Photographs were obtained using cellSens Dimension.
8 μm wells
Manufactured by Corning
Sourced in United States
The 8-μm wells are a laboratory equipment product designed for various applications. These wells have a depth of 8 micrometers, providing a controlled environment for specific experimental or analytical processes. The core function of the 8-μm wells is to offer a standardized and precise containment system for samples or reagents, enabling controlled and consistent experimentation or analysis.
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2 protocols using 8 μm wells
1
Transwell-Based Cell Invasion Assay
2
Cell Migration and Invasion Assay
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As described previously,23 (link) a cell migration assay was performed using 24-well plates, 6.5 mm chambers, and 8 μm wells (Corning Corporation, USA). Lower chambers were filled with DMEM containing 20% FBS (600 μL). The cells were digested and inoculated in DMEM containing 5% FBS at an adjusted concentration of 1×105 cells/mL. A total of 100 μL of this cell suspension was added to the upper chamber, and the membrane was carefully placed facing down on top of the cells. The cells were then fixed in 4% paraformaldehyde for 24 h at 37 °C. The cells were counted using a microscope by wiping the upper cell chamber with a cotton swab and staining the cells with 0.1% crystal violet solution.
Invasion chambers (BD Biosciences) were coated with Matrigel with a pore size of 8.0 μm in 24-well plates. Matrigel was situated facing the upper compartment and the sample was added to the lower compartment. Re-suspended tumor cells were added to the upper ventricle, where each group induced the invasive cells to penetrate the membrane. The results were evaluated after 24 h.
Invasion chambers (BD Biosciences) were coated with Matrigel with a pore size of 8.0 μm in 24-well plates. Matrigel was situated facing the upper compartment and the sample was added to the lower compartment. Re-suspended tumor cells were added to the upper ventricle, where each group induced the invasive cells to penetrate the membrane. The results were evaluated after 24 h.
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