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Smac007

Manufactured by GlaxoSmithKline
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SMAC007 is a laboratory instrument designed for analytical testing. It is capable of performing a variety of analytical techniques to support research and development activities. The core function of SMAC007 is to provide accurate and reliable data to researchers and scientists.

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Lab products found in correlation

Z vad fmk, by Enzo Life Sciences (2 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Sytox green, by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Corning (1 mentions) Fetal calf serum (fcs), by Atlanta Biologicals (1 mentions) Bms 345541, by Merck Group (1 mentions) Tnf α, by Thermo Fisher Scientific (1 mentions) Ifn β, by PBL Assay Science (1 mentions) Poly 1 c, by Cytiva (1 mentions) Casp8, by Thermo Fisher Scientific (1 mentions) Recombinant human tnf, by R&D Systems (1 mentions) Anti p mlkl, by Abcam (1 mentions) Anti fadd, by Abcam (1 mentions) Flag tagged tnf, by Enzo Life Sciences (1 mentions) Anti ripk1, by BD (1 mentions) Cycloheximide ch, by Merck Group (1 mentions) Gsk 963, by GlaxoSmithKline (1 mentions) Anti c flip, by Cell Signaling Technology (1 mentions)

2 protocols using smac007

1

Maintenance and Differentiation of Cell Lines

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HT-29, L929, NIH3T3, 3T3SA, SVEC, and MEF were maintained in DMEM containing 4.5 g/ml glucose, 10% fetal calf serum (Atlanta Biologicals), 2 mM L-glutamine with 100 units penicillin/ml and 100 units streptomycin/ml (Invitrogen). Primary neutrophils were maintained in RPMI containing 4.5 g/ml glucose, 10% fetal calf serum (SAFC Bioscience), 2 mM L-glutamine with 100 units penicillin/ml and 100 units streptomycin/ml. For BMDM cultures, pooled bone marrow cells from flushed tibias and femurs were differentiated for 5 to 7 days in DMEM containing 20% fetal calf serum and 20% filtered L929-conditioned medium (to provide macrophage colony-stimulating factor). The reagents and compounds used were IFNβ (PBL), TNFα (Peprotech), qVD-OPH, zYVAD-fmk, zIETD-fmk, zLEHD-fmk, 3-Methyadenine (3-MA) and Necrostatin (Nec)-1 (Calbiochem), non-targeting, RIP1, Casp8, and, MLKL siRNA ON-TARGET SMARTpools (Thermo Scientific), cycloheximide, butylated hydroxyanisole (BHA) and BMS-345541 (Sigma), DMSO (Corning), zVAD-fmk (Enzo Life Sciences and Promega), SYTOX Green (Invitrogen), poly(I:C) (Amersham Pharmacia), BV6 (Genentech) and SMAC007 (GlaxoSmithKline).
Mandal P., Berger S.B., Pillay S., Moriwaki K., Huang C., Guo H., Lich J.D., Finger J., Kasparcova V., Votta B., Ouellette M., King B.W., Wisnoski D., Lakdawala A.S., DeMartino M.P., Casillas L.N., Haile P.A., Sehon C.A., Marquis R.W., Upton J., Daley-Bauer L.P., Roback L., Ramia N., Dovey C.M., Carette J.E., Chan F., Bertin J., Gough P.J., Mocarski E.S, & Kaiser W.J. (2014). RIP3 induces apoptosis independent of pro-necrotic kinase activity. Molecular cell, 56(4), 481-495.
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2

Antibody Cocktail for Cell Death Signaling

Cited 1 time
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The following antibodies were from Cell Signaling Technology: anti-Casp8 (9746), anti-Cap3 (9664), anti-cIAP2 (3136), anti-pSAPK/JNK (JNK1/2, 4668), anti-pIκBα (9246), anti-Caveolin1 (3267), and anti-cFLIP (56343). The following antibodies were from Abcam: anti-pMLKL (187091), anti-RIPK3 (72106), anti-pRIPK3 (209384), and anti-FADD (108601). Anti-Ub (S.C8017), and protein A/G-conjugated beads were from Santa Cruz Biotechnology, anti-total MLKL (M6697) was from Sigma-Aldrich, anti-RIPK1 (610459) was from BD Medical Technology, and anti-cIAP1 was a gift from of John Silke (Walter and Eliza Hall Institute of Medical Research). z-VAD-fmk was from Enzo Life Sciences, RIPK1 inhibitor GSK’963, RIPK3 inhibitor GSK’840 and IAP antagonist SMAC007, as well as the pRIP1 S166-specific antibody, were provided by GlaxoSmithKline34 (link). IAP antagonist BV6 was provided by Domogoj Vucic (Genentech), recombinant human TNF was from R&D or from PeproTech, Flag-Tagged TNF was from Enzo, necrosulfonamide was from CalBiochem, TLCK (Tosyl-L-lysyl-chloromethane hydrochloride) was from Abcam, cycloheximide (CH) was from Sigma-Aldrich, and Carfilzomib was from BioVision.
Ali M, & Mocarski E.S. (2018). Proteasome inhibition blocks necroptosis by attenuating death complex aggregation. Cell Death & Disease, 9(3), 346.
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