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H 700h transmission electron microscope

Manufactured by Hitachi
Sourced in Japan

The Hitachi H-700H is a transmission electron microscope (TEM) designed for high-resolution imaging and analysis of materials. The H-700H is capable of magnifying samples up to 1,500,000 times and can achieve a resolution of 0.14 nanometers. The microscope utilizes an electron beam to illuminate and interact with the specimen, allowing for detailed examination of the internal structure and composition of materials at the atomic scale.

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2 protocols using h 700h transmission electron microscope

1

Synthesis and Characterization of Ag2S Quantum Dots

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For the synthesis of the Ag2S QDs, 400 μmol of GSH and 100 μmol of AgNO3 were added to 75.0 mL of deionized water and stirred for a few minutes. Then, 25.0 mL of Na2S solution was added under stirring. After this, the pH of the mixture was adjusted to 7.5 with NaOH and heated at 95 °C for 3 h. The Ag2S QDs were purified via centrifuge ultrafiltration (10 kDa MW).
Fluorescence spectrum was collected on a Lengguang F98 spectrophotometer. X-ray diffraction (XRD) data was obtained on a Philips X'Pert PRO XL-30 X-ray diffractometer. Transmission electron microscopy (TEM) and elemental mapping were performed on a Hitachi H-700H transmission electron microscope. Fourier transform infrared spectroscopy (FT-IR) were performed on a Thermo Nicolet 5700 instrument.
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2

Characterization of Fe3O4 Nanoparticles

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The morphology of the Fe3O4 nanoparticles were examined using a H-700H transmission electron microscope (TEM, Hitachi, Ltd., Tokyo, Japan) at an accelerating voltage of 100 kV, and the samples of Fe3O4 nanoparticles for the TEM study were prepared by placing drops of the nanoparticles in ethanol on the surface of carbon grids. The size of Fe3O4 nanoparticles was assessed by dynamic light scattering (DLS) using a DynaPro-MS800 instrument (Wyatt Technology, Santa Barbara, CA, USA), for which the Fe3O4 nanoparticles were dispersed in phosphate-buffered saline (PBS) at a concentration of 50 μg/mL. After ultrasonication, the aqueous solution was obtained after the samples were equilibrated at 25 °C for 10 min. The surface zeta potential of Fe3O4 nanoparticles was measured using a Malvern Nano-ZS90 Zetasizer Nano instrument (Malvern Instruments Ltd., Malvern, UK) at room temperature. The magnetic properties of the Fe3O4 nanoparticles were measured with a vibrating sample magnetometer (VSM, Quantum Design, Inc., San Diego, CA, USA) vibrating sample magnetometer at room temperature.
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