Cytokines concentrations were measured in serum inactivated for 20 minutes at 56°C (13 (link)) using Luminex multiplex bead-based technology (R&D Systems) and a Bio-Plex 200 instrument (BioRad), on serum diluted to 1/2.
Luminex multiplex bead based technology
Manufactured by R&D Systems
Sourced in United States
The Luminex® multiplex bead-based technology is a platform that enables the simultaneous measurement of multiple analytes in a single sample. The technology utilizes color-coded magnetic beads, each coated with a specific capture antibody, to allow for the detection and quantification of multiple targets in a single well or tube.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using luminex multiplex bead based technology
1
Multiplex Luminex Cytokine Assay
2
Quantification of Cytokines and Biomarkers in BAL Fluid
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cytokines were measured at distance using Luminex® multiplex bead-based technology (R&D Systems, Minneapolis, MN, USA) and a Bio-Plex 200® instrument (BioRad, Hercules, CA, USA), according to the manufacturers’ protocols. BAL fluid concentrations of 22 biomarkers, including inflammatory markers and cytokines/chemokines (interleukin (IL)-1Ra, IL-6, IL-7, IL-8, IL-10, IL-12/23p40, IL-13, IL-17A, interferon (IFN)-γ, tumor necrosis factor (TNF)-α, granulocyte–macrophage-colony stimulating factor (GM-CSF), RANTES, CXCL10, Serpin E1), endothelial injury (intercellular adhesion molecule-1 (ICAM-1), vascular endothelial growth factor (VEGF), von Willebrand Factor (vWF), angiopoietin (Ang)-1/2) and alveolar epithelium injury (receptor for advanced glycation end products (RAGE), surfactant protein (SP)-D, amphiregulin) biomarkers, were quantified in BAL fluid supernatant and serum and expressed in fluorescence intensities and concentrations (pg/mL).
3
Multiplex Biomarker Profiling in ARDS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Concentrations of 22 biomarkers were measured using Luminex® multiplex bead-based technology (R&D Systems, Minneapolis, MN, USA) in BAL fluid supernatant and serum from 70 pneumonia-related ARDS from the PICARD cohort [6 (link)]. These included inflammatory markers and cytokines/chemokines (interleukin (IL)-1Ra, IL-6, IL-7, IL-8, IL-10, IL-12/23p40, IL-13, IL-17A, interferon (IFN)-γ, tumor necrosis factor (TNF)-α, granulocyte-macrophage-colony stimulating factor (GM-CSF), RANTES, IP-10/CXCL10, and Serpin E1), endothelial injury (intercellular adhesion molecule-1 (ICAM-1), vascular endothelial growth factor (VEGF), von Willebrand Factor (vWF), and Angiopoietin (Ang)-1/2) and alveolar epithelium injury (receptor for advanced glycation end products (RAGE), surfactant protein (SP)-D, and amphiregulin). The results of quantification were expressed as fluorescence intensities.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!