Cortisol elisa kit

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Cortisol ELISA kit is a laboratory diagnostic tool used to measure the concentration of the hormone cortisol in biological samples. It is designed to provide accurate and reliable results for research and clinical applications.

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Lab products found in correlation

Biotek synergy ht microplate reader, by Agilent Technologies (1 mentions)

Close spelling variants of this product

Cortisol Competitive ELISA Kit Cortisol Competitive Human ELISA kit Cortisol ELISAs

2 protocols using cortisol elisa kit

Quantifying Cortisol and Dexamethasone Levels

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Cortisol levels were determined using Thermofisher cortisol ELISA kit, a solid phase competitive Enzyme-linked Immunosorbent Assay kit that detects and quantifies the total cortisol in serum. Subsequent steps complied with the manufacturer's instructions. The assay for Dexamethasone-Induced protein (used as an index of dexamethasone levels), was performed using MyBioSource® ELISA kit according to the manufacturer's instructions.
The optical density (OD) for all assays was measured at a wavelength of 450 nm corrected at 540nm on a Microplate reader (Biotek Synergy HT Microplate reader, BioTek Instruments Inc. Vermont USA).

Idowu A.O., Bhattacharyya S., Gradus S., Oyibo W., George Z., Black C., Igietseme J, & Azenabor A.A. (2018). Plasmodium falciparum Treated with Artemisinin-based Combined Therapy Exhibits Enhanced Mutation, Heightened Cortisol and TNF-α Induction. International Journal of Medical Sciences, 15(13), 1449-1457.

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Salivary Oxytocin and Cortisol Measurement

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The salivary OT levels were measured using the commercial kit Oxytocin EIA kit (Genprice Inc., San Jose, CA, USA) (Oxytocin ELISA kit, cat. No. K048). Salivary C levels were measured using the commercial kit ELISA (Thermo Fisher Scientific, Waltham, MA, USA) (Cortisol ELISA kit, cat. No. EIAHCOR). The measures were performed according to the instructions of the producer. Plates were scanned using a specialized Charge Coupled Device cooled tool. The integrated density values of the spots of known standards were used to generate a standard curve. Density values for unknown samples were determined using the standard curve for each analyte to calculate the real values in pg/mL or ng/mL. All steps were performed twice and at room temperature. The OT assay sensitivity was equal to 1.7 pg/mL, for the C it was equal to 17.3 ng/mL. The intra- and inter-assay reproducibility was > 90%. Duplicate values that differed from the mean more than 10% were considered suspect and therefore repeated. We did not reported missing values in salivary evaluation of OT and C.

La Fratta I., Franceschelli S., Speranza L., Patruno A., Michetti C., D’Ercole P., Ballerini P., Grilli A, & Pesce M. (2021). Salivary oxytocin, cognitive anxiety and self-confidence in pre-competition athletes. Scientific Reports, 11, 16877.

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