Mouse anti p α syn

Both protease and phosphatase inhibitors were purchased from Roche (Basel, Switzerland). Primary antibodies used in western blotting are as follows: Mouse anti-β-actin (1:5000, Proteintech), Rabbit anti-p-α-syn (1:1000, Abcam), Mouse anti-p-α-syn (1:1000, Wako), Mouse anti-α-syn (1:1000, Santa Cruz), Rabbit anti-Human α-syn (1:2000, Abcam), Mouse anti-demethylate-PP2A (1:1000, Millipore), Mouse anti-PP2A (1:1000, BD biosciences), Mouse anti-β-tubulin (1:1000, Abcam), Mouse anti-PPP2R2A (1:1000, CST), Mouse anti-PPP2R2B (1:1000, Abcam), Rabbit anti-PPP2R2D (1:1000, Abcam), Mouse anti-PPP2R5A (1:500, Santa Cruz), Rabbit anti-PPP2R5D (1:5000, Abcam), Rabbit anti-PPP2R5E (1:1000, Abcam), Mouse anti-LCMT-1 (1:1000, Abcam), Rabbit anti-PME-1 (1:1000, Millipore), Mouse anti-GAPDH (1:3000, Sigma), Mouse anti-c-Myc (1:5000, Clontech), and Mouse anti-FLAG (1:500, Santa Cruz). Secondary antibodies are as follows: Goat anti-Rabbit IRdye680 (LI-COR Bioscience), Goat anti-Mouse IRdye680 (LI-COR Bioscience), Goat anti-Rabbit IRdye800 (LI-COR Bioscience), Goat anti-Mouse IRdye800 (LI-COR Bioscience), Goat anti-Rabbit Alexa Fluor 488 (Thermo Fisher Scientific). The inhibitor of protein phosphatase methylesterase-1 (PME-1), AMZ30, was purchased from MedChem Express (Shanghai, China). Dulbecco’s Modified Eagle’s Medium (DMEM), and fetal bovine serum (FBS) were purchased from Gibco.