Nile red

Manufactured by HiMedia

Sourced in India

Nile red is a fluorescent dye used for the detection and quantification of lipids and lipid-rich structures in biological samples. It is a highly selective and sensitive probe for neutral lipids, such as triacylglycerols and cholesterol esters, and can be used in a variety of applications, including cell biology, biochemistry, and analytical chemistry.

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Lab products found in correlation

Picric acid, by HiMedia (1 mentions) Chloroform, by HiMedia (1 mentions) N hexane, by HiMedia (1 mentions) Fame standard, by Merck Group (1 mentions) Sodium carbonate, by HiMedia (1 mentions) Chloroform, by Merck Group (1 mentions) N hexane, by Merck Group (1 mentions) Streptomycin, by HiMedia (1 mentions) Peptone, by HiMedia (1 mentions) Malt extract, by HiMedia (1 mentions)

4 protocols using nile red

Glycation and Antioxidant Nanoparticles

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Bovine serum albumin (purity > 98%), methylglyoxal (40% in H₂O), acetylglycyl-lysine methyl ester (G.K.) peptide (purity > 98%), aminoguanidine hydrochloride (purity > 98%), zinc oxide nanopowder were obtained from SRL (India). Nile red and δ-Gluconolactone were purchased from HiMedia (India) and all other chemicals used in the study were of analytical grade.

Anandan S., Mahadevamurthy M., Ansari M.A., Alzohairy M.A., Alomary M.N., Farha Siraj S., Halugudde Nagaraja S., Chikkamadaiah M., Thimappa Ramachandrappa L., Naguvanahalli Krishnappa H.K., Ledesma A.E., Nagaraj A.K, & Urooj A. (2019). Biosynthesized ZnO-NPs from Morus indica Attenuates Methylglyoxal-Induced Protein Glycation and RBC Damage: In-Vitro, In-Vivo and Molecular Docking Study. Biomolecules, 9(12), 882.

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Lipid Profiling Using Nile Red

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All materials for culture medium and chemicals, such as n-hexane, methanol, chloroform, picric acid, sodium carbonate, and Nile red were bought from Hi-Media Laboratories (Mumbai, India). PCR primers and FAME standards were purchased from Sigma-Aldrich (St Louis, MO, USA).

Thangavelu K., Sundararaju P., Srinivasan N., Muniraj I, & Uthandi S. (2020). Simultaneous lipid production for biodiesel feedstock and decontamination of sago processing wastewater using Candida tropicalis ASY2. Biotechnology for Biofuels, 13, 35.

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Visualization of Lipid Droplets in Jatropha Leaves

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Leaf sections of transgenic and control Jatropha plants were stained with Nile red (HiMedia, India), mounted in 70 % glycerol, and visualized using a laser confocal scanning microscope. Oil droplets were observed at 570–630 nm emission following 559-nm excitation by solid-state laser. Images were captured with LSM 510 META laser scanning microscope (Lieca, Germany).

Maravi D.K., Kumar S., Sharma P.K., Kobayashi Y., Goud V.V., Sakurai N., Koyama H, & Sahoo L. (2016). Ectopic expression of AtDGAT1, encoding diacylglycerol O-acyltransferase exclusively committed to TAG biosynthesis, enhances oil accumulation in seeds and leaves of Jatropha. Biotechnology for Biofuels, 9, 226.

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Microbial Lipid Extraction and Analysis

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All the microbiological media supplements, general chemicals, and reagents like agar, malt extract, peptone, Nile red, BF 3 -methanol reagent, and streptomycin were procured from HiMedia, India. Standard FAME Mix C14-22 was procured from Sigma-Aldrich, India, and analytical grade solvents like chloroform, methanol, and n-hexane were purchased from Merck Ltd., India.

Bardhan P., Gupta K., Kishor S., Chattopadhyay P., Chaliha C., Kalita E., Goud V.V., & Mandal M. (2020). Oleaginous yeasts isolated from traditional fermented foods and beverages of Manipur and Mizoram, India, as a potent source of microbial lipids for biodiesel production. Annals of Microbiology, 70(1).

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