Yeast peptone dextrose agar (YPD, 1% yeast extract, 2% peptone, 2% dextrose, and 2% agar) supplemented with 50 μg/ml phloxine B (Wako Pure Chemical Industries, Ltd., Japan) was used for white/opaque phenotype detection. Cells were streaked onto YPD-phloxine B plates and incubated at room temperature for 2 weeks. The phenotype of colonies was observed under a stereoscopic dissecting microscope (Leica M125, Leica, Germany), and the phenotype of cells was observed under a scanning electron microscope (JSM-7200F, JEOL, Japan).
Phloxine b
Manufactured by Fujifilm
Sourced in Japan
Phloxine B is a synthetic dye used in various laboratory applications. It functions as a stain, enhancing the visual contrast and visibility of biological samples during microscopic analysis. The core purpose of Phloxine B is to provide a reliable and consistent staining solution for researchers and technicians working in the field of life sciences.
Automatically generated - may contain errors
Lab products found in correlation
Jsm 7200f, by JEOL (1 mentions)
Ethylene glycol dimethacrylate, by Merck Group (1 mentions)
N isopropylacrylamide, by Tokyo Chemical Industry (1 mentions)
Eosin y, by Fujifilm (1 mentions)
Orange 2, by Fujifilm (1 mentions)
Ethanol, by Fujifilm (1 mentions)
Sodium dodecyl sulfate (sds), by Fujifilm (1 mentions)
Rose bengal, by Fujifilm (1 mentions)
Potassium peroxodisulfate, by Fujifilm (1 mentions)
Disodium hydrogen phosphate, by Fujifilm (1 mentions)
Styrene, by Fujifilm (1 mentions)
Erythrosine, by Fujifilm (1 mentions)
Eclipse e800 microscope, by Nikon (1 mentions)
3 protocols using phloxine b
1
Yeast Phenotype Screening Protocol
2
Synthesis and Characterization of Polymeric Microspheres
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2-Methoxyethyl acrylate (MEA, purity 98%),
styrene (St, 99%), butyl acrylate (BA, 99%), potassium peroxodisulfate
(KPS, 95%), sodium dodecyl sulfate (SDS, 95%), disodium hydrogenphosphate
(99%), eosin Y (EoY, 95%), phloxine B (PhB, 98%), erythrosine (Ery,
95%), rose bengal (RB, 95%), orange II (OrII, 98%), tartrazine (Ttz,
98%), and ethanol (EtOH, 99.5%) were purchased from Wako Pure Chemical
Industries and used as received. 2-(2-Ethoxyethoxy) ethyl acrylate
(ET2A, 98%), 4-methoxystyrene (MSt, 98%), N-isopropylacrylamide
(NIPAm, 98%), and N,N′-methylenebis(acrylamide)
(BIS, 97%) were purchased from Tokyo Chemical Industry and used as
received. 2-(2-Methoxyetoxy) ethyl acrylate (ME2A, 95%) was purchased
from Monomer-Polymer and Dajac Labs, Inc. 2-[2-(2-Methoxyethoxy)ethoxy]ethyl
acrylate (Me3A, 98%) was kindly donated by Kyoeisha Chemical Co.,
Ltd. The cross-linker, ethylene glycol dimethacrylate (EGDMA, 98%),
was purchased from Sigma-Aldrich and used as received. Water used
for microsphere preparations was distilled and then ion-exchanged
(EYELA, SA-2100E1).
styrene (St, 99%), butyl acrylate (BA, 99%), potassium peroxodisulfate
(KPS, 95%), sodium dodecyl sulfate (SDS, 95%), disodium hydrogenphosphate
(99%), eosin Y (EoY, 95%), phloxine B (PhB, 98%), erythrosine (Ery,
95%), rose bengal (RB, 95%), orange II (OrII, 98%), tartrazine (Ttz,
98%), and ethanol (EtOH, 99.5%) were purchased from Wako Pure Chemical
Industries and used as received. 2-(2-Ethoxyethoxy) ethyl acrylate
(ET2A, 98%), 4-methoxystyrene (MSt, 98%), N-isopropylacrylamide
(NIPAm, 98%), and N,N′-methylenebis(acrylamide)
(BIS, 97%) were purchased from Tokyo Chemical Industry and used as
received. 2-(2-Methoxyetoxy) ethyl acrylate (ME2A, 95%) was purchased
from Monomer-Polymer and Dajac Labs, Inc. 2-[2-(2-Methoxyethoxy)ethoxy]ethyl
acrylate (Me3A, 98%) was kindly donated by Kyoeisha Chemical Co.,
Ltd. The cross-linker, ethylene glycol dimethacrylate (EGDMA, 98%),
was purchased from Sigma-Aldrich and used as received. Water used
for microsphere preparations was distilled and then ion-exchanged
(EYELA, SA-2100E1).
3
Phloxine B-Stained Microbial Imaging
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Microbes were grown on banana-agar plates for 3 days, then collected and suspended in PBS at a concentration of ~1 × 108 cells/ml. The cells were subsequently incubated in 5 µg/ml Phloxine B (Wako) for 10 min at room temperature. The cells were subsequently washed twice with PBS and observed using a Nikon C1 laser scanning confocal microscope coupled to a Nikon Eclipse E-800 microscope. Note that the brightness of the images was adjusted with a gradation to achieve mostly uniform brightness, because the bottom side of the original images was darker due to microscope malfunction.
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