Terminal deoxynucleotidyl transferase‐mediated dUTP nick end labeling (TUNEL) assay was performed using the DEADEND Fuorometric TUNEL System (Promega, Madison, WI, USA). Cells (1.0 × 105 per well) were seeded in 6‐well culture plates and incubated for 24 h. Cells were transfected with siRNAs as described, and were re‐plated to Poly‐l ‐Lysine coated glass (Matsunami Glass Ind., Osaka, Japan) inside a 24‐well culture plate. Forty‐eight hours after transfection, cells were then treated with TUNEL staining according to the manufacturer's protocol. The slides were treated with 4′6′‐diamidino‐2‐phenylindole dihydrochloride (DAPI) for nuclear staining. Signals were captured using digital microscope (VH‐8000; Keyence, Osaka, Japan). Percentage of apoptotic cells were evaluated in five randomly selected fields (×100), and data are presented as mean value ± SD.
Deadend fuorometric tunel system
Manufactured by Promega
Sourced in United States
The DEADEND Fluorometric TUNEL System is a lab equipment product designed for the detection and quantification of apoptosis, a programmed cell death process. The system utilizes the TUNEL (Terminal Deoxynucleotidyl Transferase Deoxyuridine Triphosphate Nick End Labeling) assay to label DNA fragmentation, a hallmark of apoptosis. The product provides a fluorometric-based method for the analysis of apoptotic cells.
Automatically generated - may contain errors
2 protocols using deadend fuorometric tunel system
1
Quantifying Apoptosis via TUNEL Assay
2
Apoptosis Evaluation in Transfected Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells (3 × 104) were seeded on cover glass in 24-well plates 24 hours before transfection, DTX addition, or RNase H2 i addition. After 48 hours of incubation, the DEADEND Fuorometric TUNEL system (Promega) was used to evaluate apoptosis, according to the manufacturer's protocol. 4′,6-diamino-2-phenylindole (Thermo Fisher Scientific) was used to stain the nuclei, and TUNEL-positive cells were observed by confocal laser scanning microscopy (Fluoview FV10I, OLYMPUS). The number of cells was counted in four random fields.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!