Cd28 ecd cd28 2

Manufactured by Beckman Coulter

CD28-ECD (CD28-2) is a flow cytometry reagent that detects the CD28 cell surface receptor. CD28 is a co-stimulatory molecule expressed on T cells that plays a crucial role in T cell activation and survival. The CD28-ECD (CD28-2) reagent is designed to identify and enumerate CD28-positive cells in biological samples.

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Lab products found in correlation

Cd3 apc cy7 sp34 2, by BD (1 mentions) Live dead fixable aqua, by Thermo Fisher Scientific (1 mentions) Lsrii flow cytometer, by BD (1 mentions) Cyan adp cytometer, by Agilent Technologies (1 mentions) Tcr γδ apc 11f2, by Miltenyi Biotec (1 mentions) Lsrfortessa, by BD (1 mentions)

Close spelling variants of this product

CD28 (CD28.2) ECD CD28-ECD (clone CD28-2) Anti-CD28-ECD (clone CD28.2) CD28-ECD CD28 (CD28.2)

3 protocols using cd28 ecd cd28 2

Multiparametric Flow Cytometry of Immune Cells

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Multiparametric flow cytometry was performed on PBMC, LNMC, and cells isolated from RBs using fluorescently labeled monoclonal antibodies cross-reactive in RMs. The following antibodies were used: CD3-APC/Cy7 (SP34-2), CD4-PE-CF594 (L200) CD8-BV711 (RPA-T8), CCR7-FITC (150503), CD45RA-PE/Cy7 (L48), CD95-PE/Cy5 (DX2), Ki67-AlexaFluor700 (B56), CD14-BV650 (M5E2), CD56-BV605 (NCAM16.2), CD62L-PE (SK11), HLA-DR-PerCPCy5.5 (G46.6), CCR5-APC (3A9) from BD Biosciences; CD28-ECD (CD28-2) from Beckman Coulter; CD16-BV421 (3G8), CD4-BV650 (OK-T4), PD-1-BV421 (EH12.2H7), from Biolegend; CD8-APC (DK25) from DAKO. All specimens were acquired on an LSR II (BD Biosciences) and analysis of the acquired data was performed using FlowJo software (Tree Star).

Cartwright E.K., Spicer L., Smith S.A., Lee D., Fast R., Paganini S., Lawson B.O., Nega M., Easley K., Schmitz J.E., Bosinger S.E., Paiardini M., Chahroudi A., Vanderford T.H., Estes J.D., Lifson J.D., Derdeyn C.A, & Silvestri G. (2016). CD8+ lymphocytes are required to maintain virus suppression in SIV-infected macaques treated with short-term antiretroviral therapy. Immunity, 45(3), 656-668.

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Immunophenotyping of CD4+ T Stem Cell Memory Cells

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Immunophenotyping was performed according to standard procedures and monoclonal antibodies cross-reactive in both SM and RM were used. The following antibodies were used for immunophenotyping of CD4+T_SCM in SM and RM PBMCs: Live/Dead Fixable Aqua from Invitrogen, CD14-V500 (M5E2), CD16-V500 (3G8), CD3-APC-Cy7 (SP34-2), CD45RA-APC (5H9), CCR7-PE-Cy7 (3D12), Ki67-Alexa 700 (B56), CCR5-PE (3A9), CD95-PE-Cy5 (DX2), CXCR3-PerCP-Cy5.5 (1C6/CXCR3), CD11a-FITC (HI111), CD122-Biotin (Mik-β3), streptavidin-PE, all from Becton Dickinson. CD4-BV650 (OKT4), CD8-BV711 (RPA-T8), CD27-BV570 or -BV605 (O323), streptavidin-605 all from Biolegend, CD28-ECD (CD28.2) from Beckman Coulter. Flow cytometric acquisition was carried out on an LSRII flow cytometer driven by the FACS DiVa software package (Becton Dickinson). Analysis of the acquired data was carried out using FlowJo (Tree Star) and PRISM (Graph Pad) software.

Cartwright E.K., McGary C.S., Cervasi B., Micci L., Lawson B., Elliott S.T., Bosinger S.E., Paiardini M., Vanderford T.H., Chahroudi A.M, & Silvestri G. (2014). Divergent CD4+ T memory stem cell dynamics in pathogenic and nonpathogenic SIV infections. Journal of immunology (Baltimore, Md. : 1950), 192(10), 4666-4673.

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Multicolor Flow Cytometry for T Cell Characterization

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FACS-lysed samples were thawed from liquid nitrogen at 37°C and washed with PBS containing 0.1% bovine serum albumin (BSA) (MilliporeSigma). For surface staining, cells were incubated with antibody mix at 4°C for 15–20 minutes, then washed and resuspended with 0.1% BSA/PBS. For intracellular staining, after surface staining, the Perm 2 kit (BD Biosciences) was used to permeabilize cell membranes. All samples were acquired either on CyAn ADP cytometer (Dako Cytomation) or LSRFortessa (BD Biosciences); analysis was done using FlowJo software and R.
The following antibodies were used in this study: CD3-PB (clone SP34-2, BD Biosciences), CD3-BV510 (UCHT1, BD Biosciences), TCR γδ-APC (11F2, Miltenyi Biotec), TCR Vγ9-PC5 (IMMU 360, Beckman Coulter), TCR Vδ2-FITC (IMMU 389, Beckman Coulter), CD27-PE (M-T271, BD Biosciences), CD28-ECD (CD28.2, Beckman Coulter), CD45RA-PC7 (L48, BD Biosciences), HLA-DR-V450 (G46-6, BD Biosciences), Ki-67-PC7 (B56, BD Biosciences), T-bet-BV421 (4B10, BioLegend), eomes-PE (WD1928, eBioscience, Thermo Fisher Scientific), granzyme A-PB (CB9, BioLegend), granzyme B-PE-CF594 (GB11, BD Biosciences), granulysin-PE (eBioDH2 [DH2], Invitrogen, Thermo Fisher Scientific), and perforin-PC7 (dG9, delta G9; eBioscience, Thermo Fisher Scientific).

Ma L., Papadopoulou M., Taton M., Genco F., Marchant A., Meroni V, & Vermijlen D. (2021). Effector Vγ9Vδ2 T cell response to congenital Toxoplasma gondii infection. JCI Insight, 6(16), e138066.

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