Reagentswere obtained commercially fromSigma-Aldrich and used without any further purification. The reaction was conducted at room temperature, unless otherwise noted [9 ]. The 1H and 13C NMR were recorded on a Bruker Advance II 400MHz NMR spectrometer with an indirect detection probe. Chemical shifts were reported in parts per million (ppm) from a standard of tetramethylsilane (TMS) in CDCl3 (0.1% w/v TMS) and coupling constants (J) are labeled in Hertz. Infrared spectrum was taken on a Thermo Electron Corporation IR 200 spectrophotometer and analyzed using EZ-OMNIC software.
Ir 200 spectrophotometer
Manufactured by Thermo Fisher Scientific
Sourced in Japan
The IR 200 spectrophotometer is a laboratory instrument designed to measure the absorption of infrared radiation by a sample. It is used to identify and quantify the chemical composition of materials by analyzing their unique infrared absorption spectrum.
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Lab products found in correlation
Advance 2 400mhz nmr spectrometer, by Bruker (2 mentions)
343 polarimeter, by PerkinElmer (2 mentions)
Esquire hp lc ms spectrometer, by Bruker (2 mentions)
Whatman aluminum backed plates, by Cytiva (2 mentions)
6520 quadrupole time of flight mass spectrometer, by Agilent Technologies (2 mentions)
U 3010 spectrophotometer, by Hitachi (2 mentions)
Avance 600 mhz instrument, by Bruker (1 mentions)
Gf254, by Qingdao Marine Chemical (1 mentions)
Avance 500 mhz instrument, by Bruker (1 mentions)
Nexera x2 uhplc lc 30a system, by Shimadzu (1 mentions)
J 810 circular dichroism spectrometer, by Jasco (1 mentions)
6 protocols using ir 200 spectrophotometer
1
Characterization of Organic Compounds
2
Characterization of Organic Compounds
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General Chemicals, Procedures and Instruments. The reaction was conducted at room temperature, unless otherwise noted [8 ]. Purification was accomplished by column chromatography, or high performance liquid chromatography. The 1H and 13C NMR were recorded on a Bruker Advance II 400 MHz NMR spectrometer with an indirect detection probe. Chemical shifts were reported in parts per million (ppm) from a standard of tetramethylsilane (TMS) in CDCl3 (0.1% w/v TMS). Signals in NMR spectra are defined as follows: s (singlet), d (doublet), t (triplet), m (multiplet), dd (doublet of doublets), pd (pseudo doublet), and all coupling constants (J) are labeled in Hertz. The mass spectra (MS) reported were obtained on a Bruker Esquire-HP LC/MS spectrometer in ESI+ detection mode. Samples were dissolved in methanol at a concentration of 1 mg/mL. Thin layer chromatography (TLC) was performed on oven dried Whatman aluminum-backed plates with varying eluent systems. Flash column chromatography was performed using oven dried 32 – 60 mesh 60-Å silica gel with varying eluent systems. A Perkin-Elmer 343 polarimeter was used to measure the optical rotation of all homogenous compounds. Infrared spectra were taken on a Thermo Electron Corporation IR 200 spectrophotometer and analyzed using EZ-OMNIC software.
3
Characterization of Organic Compounds
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Reagentswere obtained commercially fromSigma-Aldrich and used without any further purification. The reaction was conducted at room temperature, unless otherwise noted [9 ]. The 1H and 13C NMR were recorded on a Bruker Advance II 400MHz NMR spectrometer with an indirect detection probe. Chemical shifts were reported in parts per million (ppm) from a standard of tetramethylsilane (TMS) in CDCl3 (0.1% w/v TMS) and coupling constants (J) are labeled in Hertz. Infrared spectrum was taken on a Thermo Electron Corporation IR 200 spectrophotometer and analyzed using EZ-OMNIC software.
4
Characterization of Organic Compounds
5
Analytical Instrumentation Techniques
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The NMR spectra were obtained using an AVANCE 600 MHz instrument (Bruker Corporation, Switzerland). HRMS was determined using a 6520
quadrupole-time-of-flight mass spectrometer (Agilent, Palo Alto, CA). The UV data was obtained from a HITACHI U-3010 spectrophotometer (Hitachi Ltd, Tokyo, Japan), the IR spectrum and data obtained from IR200 spectrophotometer (Thermo Electron Corporation, Waltham, MA). In the separation process, the GF 254 (Qingdao Marine Chemical Co., Qingdao, China) was being as TLC filling, the silica gel
quadrupole-time-of-flight mass spectrometer (Agilent, Palo Alto, CA). The UV data was obtained from a HITACHI U-3010 spectrophotometer (Hitachi Ltd, Tokyo, Japan), the IR spectrum and data obtained from IR200 spectrophotometer (Thermo Electron Corporation, Waltham, MA). In the separation process, the GF 254 (Qingdao Marine Chemical Co., Qingdao, China) was being as TLC filling, the silica gel
6
Comprehensive Analytical Techniques for Compound Characterization
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The UV spectrum and data of absorbance were determined by using a HITACHI U-3010 spectrophotometer (Hitachi Ltd., Tokyo, Japan). The IR spectrum was obtained by using an IR200 spectrophotometer (Thermo Electron Corporation, Waltham, MA). The Circular dichroism spectra were obtained using J-810 Circular Dichroism spectrometer (JASCO Corporation, Japan). Optical rotations were measured with Autopol I automatic polarmeter (Rudolph Research Analytical, Hackettstown, NJ). The NMR spectra were recorded using an AVANCE 500 MHz instrument (Bruker Corporation, Switzerland). All compounds were dissolved in DMSO-d 6 or CDCl 3. Relative molecular mass were recorded by using a 6520 quadrupole-time-of-flight mass spectrometer (Agilent, Palo Alto, CA). Purity was detected on a Nexera X2 UHPLC LC-30A system (Shimadzu, Kyoto, Japan), using a Kromasil C18 column (150 mm × 4.6 mm, 5 μm, Dalian Johnsson Separation Science and Technology Corporation). The oven temperature was maintained at 40 °C. Water containing 0.1% formic acid served as solvent system A, and methanol served as solvent system B. The flow rate was 1.0 mL/min, and injection volume was 5 μL. 96-well microplate reader (HBS-1096A) was purchased from Nanjing Detie Experimental Equipment Co., Ltd. (Nanjing, China).
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