Invasion assays were carried out using QCM ECMatrix Cell Invasion Assay kit (24-well, 8 μm, colorimetric) (EMD Millipore, Billerica, MA) according to the manufacturer’s instructions. Briefly, cells were suspended in serum-free media and added to the inserts. The lower chamber was filled with serum-contained complete media. After 24 h of incubation, the invaded cells were fixed, stained, and counted.
Qcm ecmatrix cell invasion assay kit
Manufactured by Merck Group
Sourced in Germany
The QCM ECMatrix cell invasion assay kit is a laboratory tool used to measure the ability of cells to migrate through an extracellular matrix barrier. The kit provides a standardized platform for quantitative analysis of cell invasion activity.
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9 protocols using qcm ecmatrix cell invasion assay kit
1
Cell Invasion Assay Using QCM Kit
2
Cell Migration and Invasion Assay
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These studies were conducted on incubating cells with and without JM1-24-3 or irrelevant mAb (150 μg/mL) for 1 week as described previously [1 (link)]. For migration assays, 3 × 104 cells in serum-free medium (300 μL) were incubated with and without JM1-24-3 (150 μg/mL) or irrelevant mAb for 24 h. Invasion assays were carried out by a similar protocol using QCM ECMatrix cell Invasion Assay Kit (EMD Millipore).
3
Cell Migration and Invasion Assays
4
Cell Invasion Quantification Protocol
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Invasion assay was performed using QCM ECMatrix cell invasion assay kit (Merck, ECM550). In short, 300 µl cell suspension (106 cells/ml) was added at the top of the chamber in serum-free media. On the bottom, 500 µl complete media was added and the chambers were incubated at 37 °C and 5% CO2 for 72 h. Invaded cells were stained according to the manufacturer’s protocol. Phase-contrast images were taken (5–10 random fields) and quantified using ImageJ.
5
Cell Migration and Invasion Assays
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The migration and invasion assays were performed using CytoSelect 24-well wound healing assay kit (Cell Biolabs) and QCM ECMatrix cell invasion assay kit (EMD Millipore). Three individual experiments were performed for each assay.
6
Quantifying Cell Invasion Potential
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QCM ECMatrix Cell Invasion Assay kit (EMD Millipore, Darmstadt, Germany; #ECM550) was used to examine the invasive characteristics of the cells. Cells were counted and plated in equal numbers (1 Â 10 6 cells/ml) onto an invasion chamber which consisted of tissue culture plates with cell culture inserts that contain polycarbonate membranes with 8 lM pores layered with thin ECMatrix TM . During 72 hours of incubation in the nutrient gradient, invasive cells were allowed to migrate from the 0.1% FBS top layer through the thin ECM layer into the lower 10% FBS supplemented DMEM and cling to the bottom of the membrane. The invasive cells were stained with Crystal Violet and then imaged via brightfield microscopy. The number of stained cells in each well was counted and the average number of cells for each data point was calculated.
7
Quantifying Matrix Degradation in HCT116 Cells
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HCT116 cells (1 × 106) were assessed for matrix degradation using the QCM ECMatrix Cell Invasion Assay Kit (Millipore) following the manufacturer’s protocol. Images were captured using a microscope (Leica), and degraded areas were measured using ImageJ software. Area measurements were then normalized according to the number of cells.
8
QCM™ ECMatrix™ Cell Invasion Assay
9
QCM™ ECMatrix™ Cell Invasion Assay
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The QCM™ ECMatrix™ Cell Invasion Assay Kit (Millipore) was used with minor modifications. Cells were incubated in serum-free medium for 18 hr. at 37°C in 5% CO2. A cell suspension containing 5 × 105 cells/mL in serum-free medium was added to the upper chamber. Serum-containing medium was added to the lower chamber. NGP and NIH 3T3 cells were incubated for 72 and 24 hr. respectively and the protocol followed as per the manufacturer’s instructions.
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