Paraffin-embedded tissues were sectioned into 4-µm-thick slices and placed onto slides. The slides were stained with hematoxylin and eosin, periodic acid-Schiff and Masson's trichrome staining to evaluate glomerular alteration. Histological tissue damage to the kidney was observed using a Nikon ECLIPSE Ti-S inverted light microscope (Nikon Corporation). In each section, 10 renal glomerular areas were measured using ImageJ software (version 1.52; National Institutes of Health) to calculate the mean glomerular cross-sectional area. The glomerular volume was calculated using the Weibel-Gomez technique with the following formula: Glomerular volume=Area1.5 × 0.75+0.21 (22 (link)). The fibrotic area in the glomeruli was calculated using Masson's trichrome staining.
Eclipse ti s inverted light microscope
Manufactured by Nikon
Sourced in Japan
The Nikon ECLIPSE Ti-S is an inverted light microscope designed for various laboratory applications. It provides a stable and reliable platform for observing and analyzing specimens. The microscope features a series of optical components that allow for different illumination techniques, enabling users to visualize and study samples in a controlled environment.
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Lab products found in correlation
2 protocols using eclipse ti s inverted light microscope
1
Histological Assessment of Kidney Glomeruli
2
Crystal Aggregation Assay Kinetics
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Crystal aggregation assay was performed by seeding individual COM crystals into a well of 6-well plate (Corning Inc.; Corning, NY) containing the saturated aggregation buffer at different concentrations (25, 50, 100, 200, 400, and 800 μg/ml). The suspension was then trembled in a shaking incubator (Zhicheng; Shanghai, China) at 150 rpm, 25°C. After 1-h incubation, crystal morphology was examined and imaged using Nikon Eclipse Ti-S inverted light microscope (Nikon; Tokyo, Japan). Thereafter, the suspension of the aggregated crystals was transferred into a cuvette and subjected to measurement of absorbance (optical density; OD) at λ620 nm using a UV-visible spectrophotometer (Analytik Jena AG; Jena, Germany) with 10-s interval over 300 s.
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