Acs grade chloroform

HPLC-grade methanol and acetonitrile were purchased from VWR International (Mississauga, ON, Canada). ACS-grade chloroform was obtained from VWR International. Water was purified to 18 MΩ using a Barnstead Smart2Pure nanopure system (Thermo Scientific, Waltham, MA). Ammonium formate for HPLC (>99.0%) was purchased from Sigma Aldrich (Oakville, ON, Canada) and formic acid was (98% pure) was purchased from Fisher Scientific (Ottawa, ON, Canada).

All chemicals used in this work were used as received, without further purification. Copper(II) chloride dihydrate (CuCl₂·2H₂O, 99.999, 1-dodecanethiol (1-DDT, ≥98%), Fe(III) 2,4-pentanedionate (Fe(acac)₃, 97%), oleylamine (OLA, 70%), and selenium (99%) were purchased from Sigma-Aldrich (Saint Louis, MI, USA); ACS grade chloroform (99.8%) and ethanol (99.5%) from VWR International (Radnor, PA, USA); sodium sulfide (Na₂S, anhydrous) and formamide (FA, 99%) from Alfa Aesar (Tewksbury, MA, USA), ACS-reagent-grade nanopure water from Ricca chemical company (Arlington, TX, USA), and doxorubicin hydrochloride salt (DOX, >99%) from LC Laboratories (Woburn, MA, USA). Cells Counting kit-8 (CCK-8) was purchased from GLPBIO Technology Inc (Montclair, CA, USA). The Gibco Cell Culture Media and F-12K nutrient mixture were bought from Thermo Fisher (Waltham, MA, USA), and Dulbecco’s phosphate-buffered saline (DPBS) from Lonza Bioscience (Walkersville, MD, USA). The HeLa cervical cancer cells were purchased from the Antibody Research Corporation (Saint Charles, MO, USA), and the Green Fluorescence Protein (GFP)-modified lung cancer cells (A549GFP) from MyBioSource, Inc (San Diego, CA, USA).