Bulge loop mirnaqrt pcr primer kit
The Bulge-loop™ miRNAqRT-PCR Primer Kit is a laboratory tool designed for the detection and quantification of microRNA (miRNA) expression using real-time quantitative reverse transcription PCR (qRT-PCR) technology. The kit includes primers and reagents specifically formulated for the amplification and detection of miRNA molecules.
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12 protocols using bulge loop mirnaqrt pcr primer kit
Quantifying miR-146a and its Targets in Cells
lncRNA and miRNA Expression Profiling
Nrf2 and microRNA-380-3p Regulation in MPTP-Induced Neurodegeneration
Quantitative Analysis of miR-129 Expression
Quantification of miR-9 Expression
Quantitative Analysis of miR-141-3p Expression
Quantitative miR-155-3p Expression Analysis
RNA Extraction and qPCR Analysis
Quantitative analysis of mRNA and miRNA
Quantifying miRNA-21 Expression and Promoter Activity
The PPARα-promoter luciferase reporter was constructed by Longqian Biotech (China). Actively proliferating HK-2 cells were trypsinized and seeded in plates at a suitable density for routine culture. Following 24 h of incubation, transfection was carried out with Lipofectamine 3,000 (Invitrogen) as directed by the manufacturer for 48 h. This was followed by cell lysis and sample analysis with a Dual-Luciferase Reporter Assay System (E1960; Promega, USA). Renilla and Firefly luciferase activities were measured, and the ratio of Renilla luciferase activity to that of Firefly luciferase was derived. Triplicate experiments were independently repeated 3 times.
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