M2662

Manufactured by Samchun

The M2662 is a laboratory equipment designed for general use in research and scientific settings. It serves as a device for performing various analytical and experimental tasks. The core function of the M2662 is to provide a reliable and versatile platform for conducting scientific investigations, without further interpretation or extrapolation on its intended use.

Automatically generated - may contain errors

Lab products found in correlation

Methylene blue, by Samchun (3 mentions) N phenylthiourea ptu, by Merck Group (2 mentions) N phenylthiourea, by Merck Group (2 mentions)

4 protocols using m2662

Zebrafish Husbandry and Embryo Culturing

Check if the same lab product or an alternative is used in the 5 most similar protocols

Adult zebrafish were maintained with a cycle of 13‐h light and 11‐h dark in the automatic system (Genomic‐Design, Korea) at 28.5°C and pH 7.0‐7.9. The zebrafish embryos were collected by natural breeding and incubated in clean Petri dishes containing E3 medium (297.7 mM NaCl, 10.7 mM KCl, 26.1 mM CaCl₂, and 24.1 mM MgCl₂) containing 1% methylene blue (Samchun Chemicals, M2662, Korea) at 28.5°C. To inhibit formation of melanin, the zebrafish larvae were raised in the E3 medium containing 0.2 mM N‐phenylthiourea (PTU; Sigma‐Aldrich, p7629, USA). Animal subject research was reviewed and approved by the Institutional Animal Care and Use Committee at the Samsung Biomedical Research Institute and the Sungkyunkwan University.

Ki S.M., Kim J.H., Won S.Y., Oh S.J., Lee I.Y., Bae Y., Chung K.W., Choi B., Park B., Choi E, & Lee J.E. (2019). CEP41‐mediated ciliary tubulin glutamylation drives angiogenesis through AURKA‐dependent deciliation. EMBO Reports, 21(2), e48290.

+ Open protocol

+ Expand

Zebrafish Embryo Maintenance and Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols

Adult zebrafish were maintained with a cycle of 13-h light and 11-h dark in an automatic system (Genomic-Design, Korea) at 28.5°C and pH of 7.0-7.9. The zebrafish embryos were collected via natural breeding and incubated in clean petri dishes containing E3 medium (297.7 mM NaCl, 10.7 mM KCl, 26.1 mM CaCl₂, and 24.1 mM MgCl₂), with 1% methylene blue (M2662; Samchun Chemicals, Korea), at 28.5°C. To inhibit the production of melanin, which interferes with immunostaining, zebrafish larvae were raised in E3 medium containing 0.2 mM N-phenylthiourea (P7629; Sigma-Aldrich, USA). Animal research was reviewed and approved by the Institutional Animal Care and Use Committee of Samsung Biomedical Research Institute/Samsung Medical Center and Sungkyunkwan University (IACUC#20201008001 and IACUC#20200916001).

Jeong H.S., Kim H.J., Kim D.H., Chung K.W., Choi B.O, & Lee J.E. (2022). Therapeutic Potential of CKD-504, a Novel Selective Histone Deacetylase 6 Inhibitor, in a Zebrafish Model of Neuromuscular Junction Disorders. Molecules and Cells, 45(4), 231-242.

+ Open protocol

+ Expand

Zebrafish Rearing and Melanin Inhibition

Check if the same lab product or an alternative is used in the 5 most similar protocols

Adult WT zebrafish (AB strain) were maintained with a cycle of 13 h light and 11 h dark in the automatic system (Genomic-Design, Korea) at 28.5 °C and pH 7.0–7.9. The zebrafish embryos were collected by natural breeding and incubated in clean Petri dishes containing E3 medium (297.7 mM NaCl, 10.7 mM KCl, 26.1 mM CaCl₂ and 24.1 mM MgCl₂) containing 1% methylene blue (Samchun chemicals, M2662, Korea) at 28.5 °C. To inhibit the formation of melanin, which interferes with immunostaining, the zebrafish larvae were raised in E3 medium containing 0.2 mM N-phenylthiourea (PTU; Sigma-Aldrich, p7629, USA).

Patowary A., Won S.Y., Oh S.J., Nesbitt R.R., Archer M., Nickerson D., Raskind W.H., Bernier R., Lee J.E, & Brkanac Z. (2019). Family-based exome sequencing and case-control analysis implicate CEP41 as an ASD gene. Translational Psychiatry, 9, 4.

+ Open protocol

+ Expand

Transgenic Claudin K Zebrafish Study

Check if the same lab product or an alternative is used in the 5 most similar protocols

Adult Tg(claudin K:gal4‐vp16;uas:egfp) zebrafish were maintained with a cycle of 13 hours of light and 11 hours of dark in an automatic system (Genomic‐Design, Korea) at 28.5°C and pH 7.0‐7.9. The zebrafish embryos were collected by natural breeding and incubated in clean petri dishes containing E3 medium (297.7 mM NaCl, 10.7 mM KCl, 26.1 mM CaCl2, and 24.1 mM MgCl2) with 1% methylene blue (M2662, Samchun Chemicals, Korea) at 28.5°C. To inhibit the formation of melanin, which interferes with immunostaining, the zebrafish larvae were raised in E3 medium containing 0.2 mM N‐phenylthiourea (P7629, Sigma Aldrich). Animal subject research was reviewed and approved by the Institutional Animal Care and Use Committee at the Samsung Biomedical Research Institute and the Sungkyunkwan University (IACUC#20191204002).

Won S.Y., Kwon S., Jeong H.S., Chung K.W., Choi B., Chang J.W, & Lee J.E. (2020). Fibulin 5, a human Wharton's jelly‐derived mesenchymal stem cells‐secreted paracrine factor, attenuates peripheral nervous system myelination defects through the Integrin‐RAC1 signaling axis. Stem Cells (Dayton, Ohio), 38(12), 1578-1593.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!